The procedure of ethanol determination in wine by enzyme amperometric biosensor

Abstract

Aim. Development of the procedure of ethanol determination in wine by an enzyme amperometric biosensor. Methods. The amperometric biosensor method of ethanol analysis has been used in this work. Results. The paper presents comparative analysis of two methods of alcohol oxidase (AO) immobilization for development of amperometric biosensor for ethanol determination in wine. The method of AO immobilization in glutaraldehyde vapour was chosen as optimal for this purpose. The selectivity, operational and storage stability, and pH-optimum for operation of the created biosensor were determined. The procedure of ethanol determination in wine by amperometric biosensor on the basis of platinum printed electrode SensLab and AO was optimized. The analysis of ethanol concentration in wine and must samples was carried out using the developed high-stable biosensor. A good correlation between the data obtained by the biosensor and densitometry methods was shown. Conclusion. The proposed method of ethanol analysis could be used in wine productio

Catalytic fuel cell used as an analytical tool for methanol and ethanol determination. Application to ethanol determination in alcoholic beverages

“…Concerning the applications to real samples containing ethanol (commercial wines and beers), the agreement with the nominal values, reported by the producer, may generally be accepted as very good and, except in one case, better than the agreement obtained using the other two conventional enzymatic-amperometric methods and the nominal values themselves. However, also the agreement between the values obtained using the fuel cell and the other two conventional biosensor methods, the latter already extensively tested both by us [22,23] and other researchers [28][29][30], is more than satisfactory, as is shown by the correlation curves and R 2 coefficients set out in Table 7. Lastly, also the results of the statistical tests, set out in Tables 5 and 6, are more than satisfactory.…”

Practical application of electrochemical enzyme biosensors

“…Additionally, the stability of wines during storage also depends on the lactate concentration [39]. Amperometric biosensors based on individual enzymes suchas lactate oxidase, glucose oxidase, alcohol oxidase and glycerol oxidase have been developed for lactate, glucose, etha nol and glycerol detection, and their laboratory prototypes were fabricated and thoroughly tested for wine and must samples [40][41][42][43][44][45][46][47][48].…”

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“…Additionally, the stability of wines during storage also depends on the lactate concentration [39]. Amperometric biosensors based on individual enzymes suchas lactate oxidase, glucose oxidase, alcohol oxidase and glycerol oxidase have been developed for lactate, glucose, etha nol and glycerol detection, and their laboratory prototypes were fabricated and thoroughly tested for wine and must samples [40][41][42][43][44][45][46][47][48].…”