Application of Amperometric Enzyme Biosensors for Wine and Must Analysis

Abstract

The work presents application of amperometric biosensors based on platinum printed electrodes and immobilized enzymes alcohol oxidase, glucose oxidase and lactate oxidase for wine analysis. Created devices demonstrate wide dynamic range of work, good stability and high selectivity to the substrate, and are successfully applied for analysis of such complex mixtures as wine and must. Good correlation of the results of analysis of different wines and must obtained by amperometric biosensors and chromatography method is shown. Created biosensors can be used as a basis of commercial device for express analysis of ethanol, glucose and lactate in wine and must during its fermentation.

Ethanol content determination in hard liquor drinks, beers, and wines, using a catalytic fuel cell. Comparison with other two conventional enzymatic biosensors: correlation and statistical data

“…For completeness of the research, the main performances of this device were analytically compared with those of two conventional enzyme biosensors, even if based on different enzymatic reactions. As previously mentioned, this kind of biosensors is already extensively reported in the literature [14]- [16]. So those two, developed and used by us (based for instance on a different type of enzyme, but on the same enzyme immobilization method) and well tested in our previous works (see Ref.…”

“…As previously mentioned in the introduction, there is no lack of classic analytical methods, including biosensoristic methods (see for instance Ref. [14]), for ethanol determination in alcoholic beverages. However, the main objective of this research was to test a method based on a DMFC device, used so far especially for energy production purposes and to propose this alternative device also for analytical purposes.…”

A Thick-film Sensor as a Novel Device for Determination of Polyphenols and Their Antioxidant Capacity in White Wine

“…Precaution needs to be taken when selecting the dilution ratio to avoid the non-linear response. For the wine sample, a dilution ratio of 10 times is required for the glassy carbon electrode [ 16 ] and 250–2000 times for the platinum electrode [ 15 ]. The precious metals usually possess a very high activity.…”

Direct Methanol (or Ethanol) Fuel Cell as Enzymatic or Non-Enzymatic Device, Used to Check Ethanol in Several Pharmaceutical and Forensic Samples

“…In the literature there are several conventional methods for ethanol determination, i.e., titrimetrics [ 29 ] and instrumentals, for instance chromatographics [ 30 , 31 , 32 , 33 , 34 ] or spectrophotometrics [ 35 , 36 , 37 , 38 , 39 ], and in recent years also sensors-biosensor methods [ 40 , 41 , 42 , 43 , 44 ]. Our research group has recently also developed different methods for ethanol determination by using conventional biosensors.…”