Amperometric biosensor based on glycerol oxidase for glycerol determination

Горюшкина, Т. Б.; Шкотова, Л. В.; Gayda, Galina; Klepach, Halyna; Гончар, М. В.; Солдаткин, А. П.; Dzyadevych, S. V.

doi:10.1016/j.snb.2008.11.051

Cited by 39 publications

(19 citation statements)

References 15 publications

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“…The developed glycerol biosensor was characterized with a linear range of 0.05-25.6 mM and a minimum detection limit of 0.05 mM of glycerol, and the biosensor exhibited 75% of the initial activity after 15 days of storage. The biosensor was tested using wine samples with satisfactory results (Goriushkina et al 2010).…”

Section: Glycerolmentioning

confidence: 99%

Application of Enzyme Biosensors in Analysis of Food and Beverages

et al. 2011

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The importance of analyses of different parameters in food products and monitoring of a production process requires quick and reliable analytical methods and devices. For this purpose, biosensors can be a suitable option, whereas most of the current quality control techniques are time consuming, expensive, and unpractical. In this paper, we describe biosensors developed for analysis of different components present in food samples, namely, glucose, fructose, sucrose, lactose, lactic, malic, acetic, ascorbic, citric and amino acids, ethanol, glycerol, and triglyceride. Biosensors showed desirable sensitivity, selectivity, and response time required for various applications. They are often designed to avoid interference from components present in a complex sample to be analyzed.

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Section: Glycerolmentioning

confidence: 99%

Application of Enzyme Biosensors in Analysis of Food and Beverages

et al. 2011

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“…All electrochemical experiments were performed using the traditional three-electrode system in which the printed electrode SensLab (SensLab GmbH, Leipzig, Germany) combines in itself all three electrodes -platinum working, auxiliary and reference [11]. Amperometric measurements at a constant potential were carried out in 5 ml electrochemical cell using potentiostate PalmSens (Palm Instruments BV, the Netherlands).…”

Section: Amperometric Experimentsmentioning

confidence: 99%

“…Electropolymerised films can be successfully used in biosensors since these films, due to their permselectivity to hydrogen peroxide over other compounds, act as a selective barrier reducing the interfering effect of electroactive substances. More detailed characteristic of poly(3,4-ethylenedioxythiophene) (PEDT) electrochemical polymerization was published previously [11].…”

Section: Enzyme Immobilization By Electrochemical Polymerization In Tmentioning

confidence: 99%

Application of Zeolites for Immobilization of Glucose Oxidase in Amperometric Biosensors

Горюшкина

Akata

Sacco

et al. 2014

SEMST

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Abstract. An investigation was performed to evaluate the effect of different zeolites (silicalite, zeolite Y and zeolite Beta with varying Si/Al ratio) on the performance of glucose amperometric biosensor based on immobilized glucose oxidase (GOD). It was observed that detection limit of biosensors based on GOD without zeolite was 0.64 mM of glucose. However, in the case of GOD immobilization with zeolites NH 4 -Beta-25, Na-Beta and silicalites, biosensors with smaller detection limits within the range of 0.01 to 0.04 mM of glucose were obtained. The study of selectivity of designed biosensors showed that biosensor based on GOD immobilized with zeolite NH 4 -Beta-25 was much selective comparing with biosensor based on GOD without zeolites. Stability of developed devises also was investigated, and in the case of biosensor with GOD and Silicalite-1 it was higher than for GOD without zeolite. Thus zeolites of different types can be effectively used for GOD immobilization in glucose amperometric biosensors development for optimization of sensitivity, selectivity and stability of these devises. Àíîòàö³ÿ. Ìåòîþ äîñë³äaeåííÿ áóëà îö³íêà âïëèâó ð³çíèõ òèï³â öåîë³ò³â (ñèë³êàë³ò³â, öå-îë³òó Y òà öåîë³ò³â Áåòà ç ð³çíèì ñï³ââ³äíîøåííÿì ñèë³ö³é-àëþì³í³é) íà ðîáî÷³ õàðàêòåðèñ-òèêè ãëþêîçíîãî àìïåðîìåòðè÷íîãî á³îñåíñîðà íà îñíîâ³ ³ììîá³ë³çîâàíî¿ ãëþêîçîîêñèäà-çè (ÃÎÄ). Áóëî âñòàíîâëåíî, ùî ãðàíèöÿ âèçíà÷åííÿ ñóáñòðàòó äëÿ á³îñåíñîðà ç ÃÎÄ áåç öåîë³ò³â ñòàíîâèòü 0,64 ìÌ ãëþêîçè, òîä³ ÿê ïðè ³ììîá³ë³çàö³¿ ÃÎÄ ç öåîë³òàìè NH 4 -Áå-òà-25, Na-Áåòà òà ñèë³êàë³òàìè áóëè îòðèìàí³ á³îñåíñîðè ç ìåíøåþ ãðàíèöåþ âèçíà÷åí-íÿ -â³ä 0,01 äî 0,04 ìÌ. Äîñë³äaeåííÿ ñåëåêòèâíîñò³ ñòâîðåíèõ á³îñåíñîð³â ïîêàçàëî, ùî ñåíñîð íà îñíîâ³ ÃÎÄ, ³ììîá³ë³çîâàíî¿ ç öåîë³òîì NH 4 -Áåòà-25, áóâ çíà÷íî ñåëåêòèâí³øèì ó ïîð³âíÿíí³ ç ñåíñîðîì íà îñíîâ³ ÃÎÄ áåç öåîë³ò³â. Òàêîae áóëî ïðîàíàë³çîâàíî ñòàá³ëü-í³ñòü ðîçðîáëåíèõ á³îñåíñîð³â òà ïîêàçàíî, ùî âîíà áóëà âèùîþ äëÿ ÃÎÄ, ³ììîá³ë³çîâàíî¿ ç Keywords: amperometric biosensor, glucose oxidase, zeolites, glucose

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“…28). Many materials, namely, platinum (Goriushkina et al, 2010), gold (Gamella et al, 2008), glassy carbon (Guo et al, 2010), different types of graphite (Haghighi et al, 2010;PournaghiAzar et al, 2010), screen-printed electrodes (Ben Rejeb et al, 2007;Radoi et al, 2007;Ricci et al, 2007;Gurban et al, 2008), rigid carbon-polymer biocomposites (Alvarez-González et.al., 2000;Liao et al, 2008), zeolites, clays, and polymeric membranes (Katrlik et al, 2006;Rozlosnik et al, 2009) were previously used for the construction of glycerol-selective electrodes. Various strategies of enzyme immobilization (Prodromidis et al, 2002), including physical or covalent binding, gel entrapment, electropolymerization, sol-gel techniques (Gurban et al, 2008;Haghighi et al, 2010;Guo et al, 2010;Pournaghi-Azar et al, 2010;Liao et al, 2008) and self-assembled architectures (Gamella et al, 2008) were employed for biosensors construction.…”

Section: Amperometric Biosensors For Glycerol Determinationmentioning

confidence: 99%