Application of Enzyme Biosensors in Analysis of Food and Beverages

Monošík, Rastislav; Stredansky, Matuš; Tkac, Jan; Šturdı́k, Ernest

doi:10.1007/s12161-011-9222-4

Cited by 96 publications

(49 citation statements)

References 115 publications

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“…The utilization of GAO in biosensors designed for the detection of lactose in milk and other dairy products has been reviewed recently [81]. In addition to those, various other diagnostic applications of GAO have been developed; some of the most recent applications are reviewed here.…”

Section: Biosensors Diagnostics and Environmental Analyticsmentioning

confidence: 99%

Oxidation with galactose oxidase: Multifunctional enzymatic catalysis

Parikka

Master

Tenkanen

2015

Journal of Molecular Catalysis B: Enzymatic

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Section: Biosensors Diagnostics and Environmental Analyticsmentioning

confidence: 99%

Oxidation with galactose oxidase: Multifunctional enzymatic catalysis

Parikka

Master

Tenkanen

2015

Journal of Molecular Catalysis B: Enzymatic

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“…These devices are particularly useful since they combine the high specificity of the enzymes with the versatility of the transducer. In the last years, different types of enzymatic biosensors for lactose [13][14][15][16][17][18][19] and glucose [4,[20][21][22][23][24] determination have been developed for application in food industry, but few of them have been reported for the determination of both sugars. One of the first similar devices used different enzymes (invertase, -galactosidase, amyloglucosidase, mutarotase, and glucose oxidase) covalently immobilized on a cellulose membrane [25].…”

Section: Introductionmentioning

confidence: 99%

Determination of Different Saccharides Concentration by Means of a Multienzymes Amperometric Biosensor

Portaccio

Lepore

2017

Journal of Sensors

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A three-electrode amperometric biosensor for the detection of two different saccharides (lactose and glucose) in aqueous solutions is described. On the graphite working electrode, the glucose oxidase (GOD) and -galactosidase ( -gal) were coimmobilized by means of covalent bonding. The response of the biosensor as a function of the relative concentration of the two immobilized enzymes was investigated and the best working conditions were identified by measuring the sensitivity and the linear range response. In particular, our best lactose biosensor shows a linear range up to 0.010 mM and a limit of detection (LOD) and a sensitivity equal to 0.001 mM and 850 ± 81 A/mM, respectively. For glucose, the values of the above-mentioned parameters are equal to 0.015 mM for the linear range, 0.001 mM for LOD, and 505 ± 55 A/mM for the sensitivity. The working parameters of our biosensors are significant in comparison with other biosensors developed for concentration determination of the two saccharides investigated in the present work. In particular, low (LOD) and high sensitivities are obtained for lactose and glucose. To challenge our biosensor with real samples, it was tested using fruit juices, skim milk, and whey.

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“…Consequently the measurement of glutamate in biological fluids is of considerable interest. In addition, the widespread use of monosodium glutamate (MSG) in food products has led to increased interest in the use of new measurement methods for glutamate levels in food [6].…”

Section: Introductionmentioning

confidence: 99%

Development of a novel reagentless, screen-printed amperometric biosensor based on glutamate dehydrogenase and NAD+, integrated with multi-walled carbon nanotubes for the determination of glutamate in food and clinical applications

Hughes

Pemberton

Fielden

et al. 2015

Sensors and Actuators B: Chemical

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Disclaimer UWE has obtained warranties from all depositors as to their title in the material deposited and as to their right to deposit such material. UWE makes no representation or warranties of commercial utility, title, or fitness for a particular purpose or any other warranty, express or implied in respect of any material deposited. UWE makes no representation that the use of the materials will not infringe any patent, copyright, trademark or other property or proprietary rights.UWE accepts no liability for any infringement of intellectual property rights in any material deposited but will remove such material from public view pending investigation in the event of an allegation of any such infringement. The biosensor was fabricated by sequentially depositing the components on the surface of the transducer (MB-SPCE) in a layer-by-layer process, details of which are included in the paper. Each layer was optimized to construct the reagentless device.The biosensor was used in conjunction with amperometry in stirred solution using an applied potential of +0.1V (vs. Ag/AgCl). Optimum conditions for the analysis of glutamate were found to be: temperature, 35°C; phosphate buffer, pH 7 (0.75 mM, containing 0.05 M NaCl).The linear range of the reagentless biosensor was found to be 7.5 µM to 105 µM, and limit of detection was found to be 3 µM (based on n = 5, CV: 8.5% based on three times signal to noise) and the sensitivity was 0.39 nA/µM (± 0.025, coefficient of variation (CV) of 6.37%, n = 5). The response time of the biosensor was 20 -30 seconds.A food sample was analysed for monosodium glutamate (MSG). The endogenous content of MSG was 90.56 mg/g with a CV of 7.52%.The reagentless biosensor was also used to measure glutamate in serum. The endogenous concentration of glutamate was found to be 1.44 mM (n = 5), CV: 8.54%. The recovery of glutamate in fortified serum was 104% (n = 5), CV of 2.91%.

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Application of Enzyme Biosensors in Analysis of Food and Beverages

Cited by 96 publications

References 115 publications

Oxidation with galactose oxidase: Multifunctional enzymatic catalysis

Oxidation with galactose oxidase: Multifunctional enzymatic catalysis

Determination of Different Saccharides Concentration by Means of a Multienzymes Amperometric Biosensor

Development of a novel reagentless, screen-printed amperometric biosensor based on glutamate dehydrogenase and NAD+, integrated with multi-walled carbon nanotubes for the determination of glutamate in food and clinical applications

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