Application of L-lactate-cytochrome c-oxidoreductase for development of amperometric biosensor for L-lactate determination

Abstract

Aim. Development of amperometric biosensor based on L-lactate-cytochrome c-oxidoreductase (flavocytochrome b2, FC b2) for lactate determination. Methods. All experiments were performed using the amperometric method of detection. The methods of electrochemical polymerization and immobilization in glutaraldehyde vapors were used for FC b2 immobilization on the surface of electrodes. Results. The FC b2 preparation, which demonstrated the best operational characteristics after immobilization in poly (3,4-ethylen dioxythiophene), was selected. The selectivity, operational and storage stability, and pH-optimum for operation of the created biosensor were determined. The analysis of L-lactate in the model solutions and wine samples was carried outusing the developed biosensor. Conclusion. The FC b2-based biosensor due to its high stability can be effectively used for lactate determination in blood and other liquids containing no ethanol. After the selectivity optimization, the devise can be also applied for wine analysis

Direct Electron Transfer of Enzymes Facilitated by Cytochromes

“…Also a different yeast, Saccharomyces cerevisiae , was employed to create a yeast‐modified, mediated amperometric biosensor for lactic acid detection . Besides it obvious application for the determination of physiological lactate concentrations, an application for the quality control in wine is also considered …”

Elaboration of new method of enzyme adsorption on silicalite and nano beta zeolite for amperometric biosensor creation

“…GA is a polyfunctional agent which forms covalent bonds between biocatalytic particles or proteins. Therefore, the enzyme immobilization with glutaraldehyde is often used for the development of enzyme biosensors [23]. This immobilization method produces a three-dimensional matrix, in which the enzyme is closely trapped with the electrode material, thus improving both retention of the biomolecule on the electrode surface and electrical communication.…”

Practical application of electrochemical enzyme biosensors

“…Additionally, the stability of wines during storage also depends on the lactate concentration [39]. Amperometric biosensors based on individual enzymes suchas lactate oxidase, glucose oxidase, alcohol oxidase and glycerol oxidase have been developed for lactate, glucose, etha nol and glycerol detection, and their laboratory prototypes were fabricated and thoroughly tested for wine and must samples [40][41][42][43][44][45][46][47][48].…”