ABSTRAKPenelitian ini bertujuan mengetahui kadar asam laktat dan derajat asam kefir susu kambing yang difermentasikan dengan penambahan gula dan waktu inkubasi yang berbeda. Penelitian ini menggunakan rancangan petak terbagi dalam rancangan acak lengkap menggunakan dua faktor perlakuan, penambahan gula dan lama inkubasi. Susu kambing yang telah dipasteurisasi dan ditambahkan starter kefir 3% dibagi menjadi 4 kelompok, yaitu P0 sebagai kontrol, dan PI, PII, PIII sebagai kelompok perlakuan (penambahan gula masing-masing 5; 7,5; dan 10%). Kemudian masing-masing kelompok penambahan gula dibagi menjadi 4 kelompok inkubasi yaitu12, 16, 20, dan 24 jam atau suhu 37° C. Masing-masing kelompok terdiri atas 3 ulangan. Pemeriksaan kadar asam laktat dilakukan menurut Underwood (1989 ) dan pemeriksaan derajat asam dilakukan menurut Minarwati (2012). Nilai kadar asam laktat dan derajat asam dianalisis dengan analisis varian, dan dilanjutkan dengan beda nyata terkecil. Hasil penelitian menunjukkan bahwa kadar asam laktat dan derajat asam berkisar antara 0,1-7,2% dan 1,6-81,3 SH. Kadar Gula berpengaruh nyata (P<0,05) menurunkan kadar asam laktat dan derajat asam kefir, sedangkan lama inkubasi secara nyata (P<0,05 ) meningkatkan kadar asam laktat dan derajat asam kefir. Disimpulkan bahwa penambahan gula selama proses pembuatan kefir dari susu kambing tidak meningkatkan kadar asam laktat dan derajat asam kefir sedangkan lama inkubasi dapat meningkatkan kadar asam laktat dan derajat asam kefir susu kambing. ___________________________________________________________________________________________________________________ Kata kunci: asam laktat, derajat asam, kefir, susu kambing, gula PI, PII,and PIII were added with (5, 7.5, and 10% sugar). Then, each sugar added group were devided into four incubation groups, 12, 16, 20, and 24 hours at 37° C. Each group consisted of three replications. The examination of latic acid was performed according to Underwood (1989) and acidity level examination was caried out based on Minarwati (2012). The data were analyzed using Anova followed by least significant difference. The result showed that lactic content and the acidity of kefir of goat milk were ranged between 0. 1-7.2% and 1.6-81 ABSTRACT This reseacrh was proposed to know the value of lactic acid and acidity of goat milk kefir fermented by various sugar addition and incubated in different length of time. The design used in this study were split plot in completely randomized design,cosisted of two treament factors, the addition of sugar and the incubation time. Pasterized goat milk and added with 3% kefir then was grouped into 4 groups, P0 was control group,
The aim of this research was to find out in vitro antiplasmodium activity of Malacca leaves (Phyllantus emblica) ethanolic extract against Plasmodium falciparum growth. In this study, Plasmodium culture contained 5% parasitemia in ring stage was cultured using candle jar method and antiplasmodial activity test was carried out using microculture. The treatments were divided into 7 groups with four repetitions. K1 as negative control group was given Roswell Park Memorial Institute (RPMI), while K2 as positive control group was given artesdiaquine. Groups K3, K4, K5, K6, and K7 group was added with 100 µg/mL, 75 µg/mL, 50 µg/mL, 25 µg/mL, and 5 µg/mL of Malacca leaves ethanolic extract, respectively. Antiplasmodial activity was determined by inhibition concentration of 50% parasite growth (IC50). The data were analyzed using ANOVA and followed by Duncan test. The average of parasitemia level in group K1, K2, K3, K4, K5, K6, and K7 were 55.25±15.62, 8.50±2.52, 8.50±3.00, 9.25±0.95, 9.00±2.70, 9.79±2.06, and 10.75±2.22, respectively. The average of inhibition percentage in group K1, K2; K3; K4; K5; K6; and K7 were 0.00±0.00%, 84.62±4.55%; 84.62±5.43%; 83.26±1.73%; 83.71±4,90%; 82.35±3,73%; and 80.54±6.83%, respectively (P0.01). The results showed that the administration of malacca leaves ethanolic extract significantly affect (P0.01) the inhibition of Plasmodium growth as compared to group K1 (negative control). Probit analysis reveals the IC50 value was 3.889 µg/mL. In conclusion, all doses of malacca leaves ethanolic extract used in this study was able to inhibit Plasmodium falciparum growth with IC50 value was 3.889 µg/mL.
The purpose of this research was to determine the potency of methanolic extract of sernai stem (Wedelia bilflora) as analgesic to mice (Mus muculus) compared to ibuprofen. This research use split-plot method with 5 treatments and 5 replications. Group P0 as negative control was given aquadest, P1 as positive control was given ibuprofen, while P2, P3, and P4 were given the extract with dosage of 40, 50, and 60 mg/kg bw, respectively. The pain analysis of mice was conducted using hotplate method and the observation was carried out at 0 minute, 30 minutes, 60 minutes, 90 minutes, and 120 minutes. The data was analyzed using analysis of varians (Anova) followed by Duncan’s test. The results of this research showed that P1, P2, P3 and P4 had significant difference (P0,05) from P0. P1 did not have significant difference (P0,05) from P2 but it was different from P3 and P4 (P0,05). From this result we can conclude that methanolic extract of sernai stem was potential as analgesic, dosage of 40 mg/kg bw was equal to ibuprofen, while 50 mg/kg bw and 60 mg/kg bw showed better effect than ibuprofen.
ABSTRAK. Penelitian ini bertujuan mengetahui pengaruh perbedaan PGF2α analog terhadap persentase berahi sapi di Labuhanbatu Selatan. Sampel yang digunakan dalam penelitian ini adalah data hasil UPSUS SIWAB tahun 2017. Dalam penelitian ini digunakan data 2.547 ekor sapi yang memiliki variasi breed dan umur, dengan skor kondisi tubuh baik. Sapi-sapi dikelompokkan menjadi tiga kelompok berdasarkan preparat sinkronisasi berahi yang digunakan. K1= kelompok sapi yang diinjeksi dengan 5 ml PGF2α-1 (dinoprost tromethamine 5 mg/ml dan benzil alkohol 1,65%) berjumlah 1.300 ekor. K2= kelompok sapi yang diinjeksi dengan 5 ml PGF2α-2 (dinoprost tromethamine 5,5 mg/ml dan benzil alkohol 12,0 mg/ml) berjumlah 600 ekor. K3= kelompok sapi yang diinjeksi dengan 2 ml PGF2α-3 (cloprostenol 75 mg/ml dan chlorocresol 1,0 mg/ml) berjumlah 647 ekor. Penyuntikan dilakukan secara intramuskulus, dua kali dengan interval 10 hari. Data yang diperoleh kemudian dianalisis menggunakan uji Kruskal Wallis dan dilanjutkan dengan uji Mann Withney. Hasil analisis menunjukkan persentase berahi pada kelompok 1; 2; dan 3 masing-masing 80,7%; 50% dan 61,8% (P0,01). Dari hasil penelitian dapat disimpulkan bahwa PGF2α-1 mempunyai efektivitas lebih baik dibandingkan PGF2α-2 dan PGF2α-3 dalam induksi sinkronisasi berahi pada sapi di Kabupaten Labuhanbatu Selatan, Sumatera Utara. (Effectiveness of different commercial prostaglandin hormone administration on the percentage of estrous in cattles in South Labuhanbatu, North Sumatera) ABSTRACT. The aim of this study was to determine the effect of differences PGF2α analogues on the percentage of estrus in cattles in South Labuhanbatu. The sample used in this study was the 2017 SIWAB UPSUS data. Data of 2,547 cattles with various breed and age as well as in good body condition scores were selected. The cattles were grouped into three data groups based on the hormone used for estrus synchronization. The cattles in data group I (1300 cows) were injected with 5 ml PGF2α-1 (dinoprost tromethamine 5 mg/ml and benzyl alcohol 1.65%). The cattles in data group II (600 cows) were injected with 5 ml PGF2α-2 (5.5 mg/ml dinoprost tromethamine and 12.0 mg/ml benzyl alcohol). The cattles in data group III (647) were injected with 2 ml of PGF2α-3 (cloprostenol 75 µg/ml and chlorocresol 1.0 mg/ml). Injections were carried out intramuscularly, twice at 10-day intervals. The data obtained were then analyzed Kruskal Wallis and continued with Mann Withney test. The results showed that the percentage of estrus in groups I; II; and III were 80.7%; 50% and 61.8% (P0.01). From the results of the study it can be concluded that Lutalyse has better effectiveness than Capriglandin and Sincrovall in the induction of synchronization estrus in cattle in South Labuhanbatu, North Sumatera.
This study aimed to determine the analgesic potential of sernai leaves n-Hexan extract on 24 male mice, aged 2-3 month with 20-25 g weighed. This study used split-plot design consisted of 6 groups. P0 as negative control was given aquades and P1 as positive control was given ibuprofen (10 mg/kg Body Weight). Treatment groups including P2, P3, P4, and P5 were given n-Hexan extract of sernai leaves with dosage of 15, 30, 45, and 60 mg/kgBW, respectively. Pain assessment was conducted using Hot Plate method. Observation of mice behaviour including licking it's front and back leg, or by jumping. This study showed there was significant difference (P0,05) between dosage and time. Duncan’s test showed P1 was significantly different (P0,05) from P2, but P3 and P5 were not significantly different from P1. P3 and P4 were significantly different (P0,05) from P2 and not significantly different from P1, while P5 was not significantly different from P3 and significantly different from P1. We can conclude that dosage of 30 and 45 mg/kgBW of n-Hexan extract of sernai leaves had analgesic potential on mice, and 60 mg/kgBW was comparable to ibuprofen as pain relieve in mice
Pada cetakan pertama ini pembahasan difokuskan kepada tentang sejarah pengembangan obat tradisional di Indonesia, juga mengkaji hasil hasil penelitian terhadap tumbuhan Wedelia biflora. Selain itu pada buku ini juga dijelaskan bagaimana menjaga mutu daripada simplisia dan ekstrak tumbuhan yang dapat dimanfaatkan untuk pengembangan obat, khususnya obat untuk hewan. Oleh karena itu, saya mendukung penuh penerbitan buku ajar ini karena dapat bermanfaat bagi mahasiswa dan juga memberikan nilai tambah bagi pengembangan komponen pembelajaran. Efektifitas pembelajaran akan meningkat bila pada proses pembelajaran tersedia media pembelajaran, seperti buku ajar. Buku ajar dapat sebagai referensi bagi mahasiswa kedokteran Hewan ataupun bidang lain yang tertarik untuk mengembangkan tumbuhan sebagai bahan obat.
The aims of this research were to compare the physical charateristic of meat with or without the addition of curry leaf and to find out the organoleptic of meat added with curry leaf. This research used complex randomized design method. 500 grams beef sample were cut into square-shape at 2x2x2 cm and divide into 3 groups and then added a curry leaf infuse with different concentration of 0%, 25%, and 50%. Then, the meat was boiled at 70-80oC for 45 minutes. The parameters observed were pH, water holding capacity, cooking loss, and organoleptic (colour, taste, and aroma). The result showed that the use of curry leaf infuse in various concentration did not have significant effect on pH, water holding capacity, and cooking lost, but significantly effect (P0,05) on organoleptic value. Fresh meat that was added the curry leaf infuse 50% was well-liked for the colour, taste, and aroma compare to the meat without the addition of curry leaf infuse.
This research aimed to determine the total amount of Staphylococcus aureus (S. aureus) contamination in beef sold in traditional market in Banda Aceh. Total of 16 beef samples were used in this research, 2 samples from Beurawe market, 10 samples from Peunayong market, and 4 samples from Seutui market. Samples were cultured on manitol salt agar. The number of S. aureus colonies was calculated using colony counter, and the result was compared to the limit of microbial contamination based on Indonesian National Standard (SNI). The result of this research showed that the highest level of S. aureus contamination was derived from Beurawe market. It is concluded that all beef sold by seller from traditional market in Banda Aceh were contaminated by S. aureus.Key words: Staphylococcus aureus, meat, traditional market, number of contamination
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