ABSTRAKPenelitian ini bertujuan mengetahui infeksi bakteri Escherichia coli pada anak ayam kampung (Gallus domesticus). Sampel yang digunakan dalam penelitian ini adalah feses anak ayam kampung yang diperoleh dari Pasar Lambaro, Aceh Besar. Sampel diambil dengan cara swab bagian kloaka anak ayam dengan menggunakan swab steril, kemudian sampel dimasukkan ke dalam microtube, dan dibawa ke laboratorium untuk dilakukan uji selanjutnya. Penelitian ini dilakukan dengan tiga kali pengambilan sampel dengan jangka waktu satu minggu dengan jumlah sampel uji sebanyak 15 sampel. Pengambilan sampel dilakukan dengan menggunakan metode Carter. Data yang diperoleh dianalisis secara deskriptif. Hasil pemeriksaan bakteri Escherichia coli (E. coli) yang telah dilakukan pada 15 sampel diketahui bahwa 9 sampel positif terinfeksi bakteri E. coli sedangkan 6 sampel lain positif bakteri coli form lainnya. Disimpulkan bahwa bakteri E. coli menginfeksi anak ayam kampung di Pasar Lambaro Aceh Besar. ____________________________________________________________________________________________________________________ Kata kunci: Escherichia coli, Gallus domesticus, infeksi ABSTRACT The aim of this research was to find out infection of Escherichia coli (E. coli) bacteria on feses of chick (Gallus domesticus). Feses of chick from Lambaro market, Aceh
Avian influenza virus H5N1 infections are an important cause of diseases in humans and several animal species, including birds. The present study conducted to investigate the seroprevalence Avian Influenza H5N1 in native birds from 15 sub-districts of North Aceh. This study utilized 1108 serum samples collected from the axilaris vein (left or right) of birds. The standard Hemaglutination Inhibition (HI) assay was conducted at Microbiology Laboratory Faculty of Veterinary Medicine of Syiah Kuala University to determined serum antibody possitive or negative reaction against Avian influenza H5N1. The result showed that seroprevalence Avian influenza H5N1 virus was 4,7 % in North Aceh District. There were nine sub-districts were tested positively by HI test. However, the serum collected from six sub-districts did not react (negative) against Avian influenza H5N1. Based on the results we obtained, a conclusion that natural infection by Avian influenza virus in native birds occured in part of North Aceh District.
Biofilm is one of parameters for determining pathogenic pathways of a bacterium. Bacterial virulence activities can be attenuated by antibiotics with inhibiting biofilm. The aim of this study was to examine the characteristics and activities of antibiotics against the biofilm of Stapylococcus aureus isolate which taken from Aceh cattle prepuce. The study was exploratory and experimental, using a Completely Randomized Design (RAL) factorial pattern using three types of antibiotics, three concentrations and replications. Antibiotics were tetracycline, oxytetracycline and phosphomycin with concentrations of 10%, 20% and 30% (mg/mL), respectively. Therefore, the aim of this study was to examine the characteristics and activities of antibiotics to the biofilm of Stapylococcus aureus from Aceh cattle prepuce isolate. U-bottom polystyrene 96 wells microplate and Nutrient Broth (NB) medium were used to biofilm. Bacterial while biofilm was stained on crystals violet 1%. Optical Density (OD) of biofilm were λ 595 nm. Data were analyzed by using Analysis Of Variance (ANOVA). The results showed that the activity of Staphylococcus aureus was moderately positive, i.e 2,667cell/μL. It showed that antibiotics and concetrations have significant effect (P<0.05) to inhibit biofilm staphylococcus aureus. In additioon, tetracycline 30% (mg / mL) has the best ability to inhibit biofilms from 52,18% to 1,262 cell/μL. Based on that fact, it can be concluded that the virulence of Staphylococcus aureus was moderate positive to be false positive. Keywords: biofilm, antibiotics, Staphylococcus aureus, cows aceh. Abstrak Biofilm merupakan salah satu parameter untuk menentukan jalur patogenisitas suatu bakteri. Antibiotik dapat melemahkan aktivitas virulensi bakteri dengan menghambat pembentukan biofilm. Penelitian ini bertujuan untuk melihat karaksteristik dan aktivitas antibiotik terhadap biofilm Stapylococcus aureus isolat preputium sapi Aceh. Penelitian bersifat eksplorasi dan eksperimental, menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan menggunakan tiga jenis antibiotik, tiga konsentrasi dan tiga kali ulangan. Antibiotik yang digunakan adalah tetrasiklin, oksitetrasiklin dan fosfomisin dengan konsentrasi masing-masing 10%, 20% dan 30% (mg/mL). Pengujian biofilm dilakukan pada media Nutrient Broth (NB), menggunakan mikroplat U-bottom polystyrene 96 wells. Pewarnaan biofilm menggunakan kristal violet 1%. Optical Density (OD) biofilm dibaca pada λ 595 nm. Data hasil penelitian dianalisis dengan menggunakan Analysis Of Variance (ANOVA). Hasil penelitian menunjukkan aktivitas virulensi Staphylococcus aureus isolat preputium sapi Aceh berdasarkan karaksteristik biofilm tergolong positif moderat, yaitu 2,667cell/µL. Uji Biofilm menunjukkan jenis dan konsentrasi antibiotik berpengaruh secara nyata (P<0,05) terhadap daya hambat biofilm Staphylococcus aureus isolat preputium sapi Aceh. Tetrasiklin 30% (mg/mL) mempunyai kemampuan paling baik menghambat pembentukan biofilm Staphylococcus aureus isolat preputium sapi Aceh sebesar 52,18% menjadi 1,262 cell/µL, sehingga aktivitas virulensi Staphylococcus aureus isolat preputium sapi Aceh berdasarkan karaksteristik biofilm dari positif moderat menjadi positif palsu. Kata Kunci: biofilm, antibiotik, Staphylococcus aureus, sapi aceh.
ABSTRAKPenelitian ini bertujuan mengetahui titer antibodi pada serum ayam kampung (Gallus domesticus) terhadap virus Newcastle disease (ND). Sampel penelitian adalah serum ayam kampung yang diperoleh di beberapa pasar tradisional Banda Aceh. Parameter yang diamati dalam penelitian ini adalah titer antibodi serum ayam kampung dari uji hemaglutinasi inhibisi (HI) dengan memperhatikan endapan eritrosit. Data hasil pengamatan dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa dari 120 sampel yang diambil di beberapa pasar tradisional di kota Banda Aceh hanya 15 sampel (12,5%) menunjukkan protektif sedangkan 105 sampel (87,5%) tidak protektif. Tingkat proteksi ayam terhadap virus ND di pasar tradisional di kota Banda Aceh tergolong sangat rendah. ____________________________________________________________________________________________________________________ Kata kunci: antibodi, ayam kampung, Newcastle disease, uji HA/HI ABSTRACT This study aimed to determine serum antibody titer of native chicken (Gallus domesticus) against virus Newcastle disease (ND). Sera were taken from native chickens that were traded in Banda Aceh traditional markets. Methods used were HA and HI tests. Reading of the results is done by observing the erythrocyte sedimentation. Parameters observed in this study were antibody titer of native chicken to sediment erythrocytes in haemaglutination inhibition test (HI). The data were analyzed descriptively. The results showed that of the 120 samples taken in some traditional markets in the city of Banda Aceh only 15 samples (12.5%) showed a protective titer while 105 samples (87.5%) was not protective. Levels protection of native chickens against ND virus in a traditional market in the city of Banda Aceh is very low. ____________________________________________________________________________________________________________________ Key words: antibodies, native chicken, Newcastle disease, HA/HI test PENDAHULUANAyam kampung atau yang lebih dikenal dengan ayam buras (bukan ras) merupakan salah satu plasma nutfah yang mempunyai potensi penggerak ekonomi perdesaan (Wibowo dan Amanu, 2010). Hal tersebut dikarenakan ayam kampung memiliki beberapa kelebihan dibandingkan ayam-ayam jenis lain seperti pemeliharaannya sederhana, memerlukan modal yang sedikit sehinga dapat dibudidayakan dalam skala rumah tangga, mudah dipasarkan, serta komoditas telur dan daging ayam buras mempunyai harga yang relatif stabil dan selalu lebih tinggi dibandingkan dengan ayam ras petelur (Darmono, 2001).Sistem pemeliharaan ayam kampung biasanya dilakukan secara tradisional dan semi intensif. Secara tradisional ayam kampung dipelihara dengan cara dibiarkan lepas, tanpa memperhatikan aspek teknis dan perhitungan ekonomi usahanya. Pemeliharaan bersifat sambilan, pakan ayam buras tidak disediakan secara khusus hanya mengandalkan sisa-sisa hasil pertanian. Petani juga memberikan dedak padi tetapi tidak secara teratur. Sistem perkandangan kurang diperhatikan, ada yang dikandangkan didekat dapur, dan ada yang hanya berten...
This study aimed to isolate and identify the bacteria Salmonella sp in spleen of male layer chicken that died in farms Sibreh, Aceh Besar. A sample of the research is the spleen of male layer chickens. Isolation of Salmonella sp performed by inoculating spleen that were pierced using a swab on the media Selenite Cystine Broth (SCB) then planted on selective media Salmonella Shigella Agar (SSA) and colony morphology was observed, continued with observation of Gram stain under microscopic. Furthermore, colonies of suspected Salmonella sp planted in the media MR-VP (Oxoid), Indol (Difco), Simmons's Citrate Agar (Oxoid), Triple Sugar Iron Agar (TSIA), Sulfite Indol Motiliti (SIM), and media sugars (sucrose, manitol, glucose, lactose , maltose). The results of this study were analyzed descriptively. The research shows a results from a total of 33 samples, we found that 15 samples are positive that can be isolated and identified as Salmonella sp. Therefore it can be concluded that Salmonella sp is possible the death cause of male layer chicken in farms Sibreh, Aceh Besar
The aim of this study was to isolate and identify Aeromonas sp from pet shops in Banda Aceh. A total of 15 goldfish were obtained from 3 pet shop in Banda Aceh and examined at the Laboratory of Microbiology, Faculty of Veterinary Medicine, University of Syiah Kuala in Banda Aceh. Isolation of bacteria obtained from liver, kidneys and spleen were cultured in the media Rimler-Shotts (RS) Medium Base. The bacteria were identified based on morphology of the growing bacteria, Gram stain, IMViC test, and biochemical tests. The results showed that there were no Aeromonas sp in goldfish (Carrasius auratus) from three pet shops in Banda Aceh.
This study aimed to isolate, identify, and sensitivity tested of Staphylococcus aureus from post-surgery wound of local dogs.This study was held in Microbiology Laboratorium of Veterinary Faculty of Syiah Kuala University, Banda Aceh from April to May 2016. The samples were 10 dogs in their 3rd day’s treatment after surgery.The samples were acquired using sterile swab on the wound that was planted on Nutrient Broth (NB), incubated at 37°C for 24 hours, then transferred onto Mannitol Salt Agar (MSA), incubated at 37°C for 24 hours, and after that it was identified with Gram staining, catalase test, sugar test (mannitol and glucose), Metil Red-Voges Proskauer (MR-VP) test and antibiotic sensitivity test. The result of Gram staining showed 7 positive results which identified as coccus formed and coloured as purple. The catalase test showed 7 positive results, the sugar test (mannitol and glucose) showed 4 positive results, the MR-VP showed 4 positive on MR and all negative on VP.. In conclusion, 4 out of 10 samples showed a presence of Staphylococcus aureus on post-surgery wound of local dogs.This bacterium was sensitive on Tetracycline (50%) and also on Streptomycine and Gentamicin (100%). This bacterium resistant to Tetracycline (50%) and Ampicillin (100%).
Prior to the novel coronavirus, the subtype H5N1 that emerged in Asia in 2003 and hit Indonesia from 2005 to 2010 has created fears with the public. Attention has not only been detections of domestic fowls but pigeons as well. This study aimed to detect the specific antibody to the avian influenza virus of the H5N1 subtype in pigeons. We collected 20 serum samples of unvaccinated pigeons and tested them using the haemagglutination inhibition test (HI-test). This conventional test is considered as a gold standard, with a specific antigen of the avian influenza virus and a one percent solution of chicken red blood cells as a pre-treatment. The results reported of 20 samples have no specific antibody to the H5N1 subtype of avian influenza. Therefore, It can be concluded that none of the pigeons was infected by the H5N1 subtype of avian influenza virus.
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