Seroprevalensi Avian influenza H5N1 pada Unggas di Kabupaten Aceh Utara

Abstract

Avian influenza virus H5N1 infections are an important cause of diseases in humans and several animal species, including birds. The present study conducted to investigate the seroprevalence Avian Influenza H5N1 in native birds from 15 sub-districts of North Aceh. This study utilized 1108 serum samples collected from the axilaris vein (left or right) of birds. The standard Hemaglutination Inhibition (HI) assay was conducted at Microbiology Laboratory Faculty of Veterinary Medicine of Syiah Kuala University to determined serum antibody possitive or negative reaction against Avian influenza H5N1. The result showed that seroprevalence Avian influenza H5N1 virus was 4,7 % in North Aceh District. There were nine sub-districts were tested positively by HI test. However, the serum collected from six sub-districts did not react (negative) against Avian influenza H5N1. Based on the results we obtained, a conclusion that natural infection by Avian influenza virus in native birds occured in part of North Aceh District.

Seroprevalensi Penyakit Avian Influenza Subtipe H5n1 Pada Ayam Buras Di Pasar Beringkit Dan Galiran, Bali

“…Sampel darah diambil melalui vena brachialis yang sebelumnya telah didesinfeksi menggunakan kapas beralkohol 70% 1,5 ml dengan menggunakan disposable syringe volume 3 ml. Setelah darah masuk ke spuit, disisakan ruang kosong pada spuit, kemudian spuite ditempatkan di dalam cool box dengan posisi horizontal (Darmawi et al, 2013).…”

“…Sampel diambil dari ayam buras yang berumur 3 bulan keatas sehingga maternal antibodi sudah tidak ada, dan untuk melihat terjadinya infeksi pada ayam buras tersebut. Faktor-faktor yang dapat menyebabkan titer antibodi rendah yaitu ketika ayam buras terinfeksi virus HPAI dengan jumlah antigen sedikit sehingga tubuh kurang mampu membentuk antibodi (Darmawi et al, 2013). Selain itu, kemungkinan karena ayam sudah lama terinfeksi alami sehingga titer antibodinya sudah menurun.…”