An important approach of controlling against Avian Influenza should be determined to detect the antibody titres of bird flu caused by Influenza virus H5N1 in Indonesia. The aim of the present study was to detect the antibodies to Avian Influenza in serum of native chickens. This study utilized 123 serum samples collected from the axilaris vein (left or right) of native chickens. Antibody titres were examined using Hemaglutination Inhibition (HI). The result showed that indication of natural infection by Avian Influenza (H5N1) in native chickens, as shown that out of 123 serum samples, 16 (13,01%) were tested positive by HI, while only 10 (8,13%) were tested protective to Avian influenza infection. Based on the results we obtained, a conclusion that natural infection by Avian influenza virus stimulated variety level of formation antibody titres in native chickens.
This study aimed to determine the inhibitory effect and the concentration of fingerroot extract to inhibit Staphylococcus aureus growth. The data was analyzed descriptively. Ampicillin was used as positive control, distilled water was used as negative control, and the treatments were given fingerroot extract with a concentration of 5%, 15%, 25%, 35% and 45%. This study was conducted with three replications. The parameter measured was the diameter of inhibition zone formed by diffusion method. The average diameter of inhibition zone of the fingerroot extract were 15% : 10.3 mm; 25% : 13,6mm; 35% : 18,7mm; 45% ± 21,1mm, and at a concentration of 5% the inhibition zone is not formed. The final conclusion is that the fingerroot extract can inhibit the growth of Staphylococcus aureus. The higher concentration of fingerroot extract, the more extensive inhibition zone formed.
Avian influenza virus H5N1 infections are an important cause of diseases in humans and several animal species, including birds. The present study conducted to investigate the seroprevalence Avian Influenza H5N1 in native birds from 15 sub-districts of North Aceh. This study utilized 1108 serum samples collected from the axilaris vein (left or right) of birds. The standard Hemaglutination Inhibition (HI) assay was conducted at Microbiology Laboratory Faculty of Veterinary Medicine of Syiah Kuala University to determined serum antibody possitive or negative reaction against Avian influenza H5N1. The result showed that seroprevalence Avian influenza H5N1 virus was 4,7 % in North Aceh District. There were nine sub-districts were tested positively by HI test. However, the serum collected from six sub-districts did not react (negative) against Avian influenza H5N1. Based on the results we obtained, a conclusion that natural infection by Avian influenza virus in native birds occured in part of North Aceh District.
The purpose of this study was to detect the existence of the Streptococcus sp bacteria in the horses’ dental caries in Aceh Tengah Regency. The caries samples used in this study were taken from 8 horses in Aceh Tengah Regency. The samples were collected using swab on the teeth and then put into Nutrient Broth (NB) media and further were cultured on selective media TSY20B. The bacteria identification was conducted by observing the bacteria’s morphology, Gram staining and biochemical test. The eight samples of the horses’ dental caries showed that Streptococcus sp bacteria could be isolated from all of the collected samples. From this research it could be concluded that Streptococcus sp bacteria was found in the horses’ dental caries and it was one of the causes of the dental caries.
ABSTRAK. Penelitian ini bertujuan mengisolasi Candida sp. dan Aspergillus sp. pada tembolok ayam ras dan buras. Sampel dalam penelitian ini adalah tembolok ayam ras dan buras masing-masing berjumlah 15 sampel yang diambil secara acak dari tempat pemotongan unggas Peunayong Banda Aceh. Isolasi Candida sp. dan Aspergillus sp. dilakukan sesuai dengan metode Thompson (1969). Sampel dicuci dengan aquades steril yang diberi antibiotik selanjutnya ditanamkan pada media Sabouraud’s Dextrose Agar (SDA) kemudian diinkubasikan pada suhu kamar selama 2-7 hari. Pengamatan morfologi Candida sp. dan Aspergillus sp. diamati secara makroskopis. Koloni yang diduga Candida sp. dan Aspergillus sp. diperiksa secara mikroskopis. Data yang diperoleh dianalisis secara deskriptif. Hasil pemeriksaan menunjukkan bahwa Candida sp. dapat diisolasi pada semua sampel (100%) tembolok ayam ras dan ayam buras. Aspergillus sp. dapat diisolasi pada 2 dari 15 (13,33%) sampel tembolok ayam ras dan 6 dari 15 (40%) sampel tembolok ayam buras. Kesimpulan penelitian ini adalah Candida sp. tidak ada perbedaan pada tembolok ayam ras dan ayam buras sedangkan Aspergillus sp. pada tembolok ayam buras lebih banyak dari pada ayam ras. (Isolation of Candida sp and Aspergillus sp. from crops (Ingluviens) of broiler and indigenous chicken in Peunayoung market, Banda Aceh) ABSTRACT. This research aimed to isolate Candida sp. and Aspergillus sp. from crop of chicken race (broiler) and indigenous chicken. This research used crops of the chicken race (broiler) and indigenous chicken, each animal consists of 15 animals taken randomly from the poultry of slaughter house Peunayong Banda Aceh. Isolation of Aspergillus sp. was done based on Thompson method (1969). The samples were washed with sterile aquadest containing antibiotics before implanted on Sabouraud’s Dextrose Agar (SDA), then incubated at room temperature for 2-7 days. The plate was observed from Candida sp. and Aspergillus sp. colony macroscopically and microscopically. Data were analyzed descriptively. The result showed that Candida sp. Found in all samples (100%) and Aspergillus sp. Found in 2 out of 15 (13,33%) crops samples in chicken race (broiler) and 6 out of 15 (40%) crops in indigenous chicken. The conclusion is, candida was found in both chickens race (broiler) and domestic chicken, while aspergillus was found more in indigenous chicken than chickens race broiler.
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