Deteksi Antibodi Serum Terhadap Virus Avian influenza pada Ayam Buras

Darmawi, Darmawi; Manaf, Zakiyah Heryawati; Darniati, Darniati; Fakhrurrazi, Fakhrurrazi; Abrar, Mahdi; Erina, Erina

doi:10.17969/agripet.v12i1.283

Cited by 5 publications

(6 citation statements)

References 3 publications

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“…In this condition, the chickens will be susceptible to a virulent AI virus, with mortality can be reached up to 100% (Swayne and Suarez, 2000). This antibody variation titers can be influenced by several conditions including the health of chickens, the type and amount of virus that infects, as well as the difference period or the phase of infection when blood samples are taken (Darmawi et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

Seroprevalence and detection of H5N1 avian influenza virus in local chickens in Tabanan Regency, Bali, Indonesia

et al. 2021

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Aim: Avian Influenza (AI) is a zoonotic disease that causes death in poultry and humans. Monitoring the virus needs to be carried out continuously to prevent outbreaks of the disease. Seroprevalence and detection of H5N1 and H9N2 AI virus antigen were intended to monitor the presence of viruses in local chickens in Tabanan, a Regency of the Indonesian island Province of Bali. The research aims were to detect the presence of H5N1 AI virus, and to know the distribution of this virus in Tabanan Regency. Materials and Methods: Research located in six districts of Tabanan regency namely Baturiti, Penebel, Marga, Kediri, Tabanan, and Kerambitan. A total of 1,398 local chickens that never been vaccinated with AI were randomly sampled in this study. The samples collected were serum, cloacal and tracheal swabs. Serum samples were tested with hemagglutination inhibition (HI) assay. While samples of cloacal and tracheal swabs were isolated in 9-day-old germinated chicken eggs, followed by hemagglutination assay and RT-PCR test using H5N1 and H9N2 primers. Results: AI seroprevalence in local chickens in Tabanan Regency was 1% with the distribution in each district as follows; Penebel 1.6%, Kerambitan 1.2%, Marga 1%, while Tabanan, Kediri, and Baturiti 0.7% each. H5N1 AI virus was detected in 11 samples, i.e. five in Marga district and three in Penebel district, two in Kediri, and one in Tabanan, while the H9N2 AI virus was not detected. Conclusion: These results indicate that H5N1 AI virus may still circulate in local chickens in Tabanan Regency, Bali.

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Section: Resultsmentioning

confidence: 99%

Seroprevalence and detection of H5N1 avian influenza virus in local chickens in Tabanan Regency, Bali, Indonesia

et al. 2021

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“…Lamanya virus menginfeksi ayam juga mempengaruhi titer antibodi. Hewan yang baru saja terpapar virus (jangka waktu pemaparan singkat) akan menunjukkan nilai titer yang rendah, karena jumlah virus masih sedikit sehingga hewan tidak mampu menghasilkan antibodi dalam jumlah yang cukup tinggi (Darmawi et al, 2012). Mahardika et al (2016) menyatakan bahwa uji HI pada suatu serum dengan titer antibodi 4 unit HI (2 2 ) menunjukkan hasil positif rendah.…”

Section: Analisis Dataunclassified

Seroprevalensi Penyakit Avian Influenza Subtipe H5n1 Pada Ayam Buras Di Pasar Beringkit Dan Galiran, Bali

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Suartha

2021

Jurnal Sain Veteriner

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Avian Influenza (AI) is a strategic communicable and zoonotic disease. The cause is a virus with Highly Pathogenic Avian Influenza (HPAI) subtype H5N1. The poultry market has important roles in the preservation, propagation, and spreads of the Avian Influenza (AI) virus from poultry to other species and humans. The purpose of this study was to determine the level of seroprevalence of Avian Influenza (H5N1) in free-range chickens at the Beringkit and Galiran market. A total of 120 free-range chickens were used as a sample. They have taken 60 serum samples per market. Serum removal is made from 5 merchants under the provisions of 3 samples from merchants who sell 6 to 10 free-range chickens. The sampling period is carried out for 2 months every 2 weeks 4 times. Sample testing was performed at the Denpasar Veterinary Centre with Haemagglutination (HA) and Haemagglutination Inhibition (HI) as barriers. The data titer of the antibodies obtained was analyzed by Non-Parametric Statistic Test Chi-Square (χ2) using IBM SPSS for windows. The results of the study showed that the AI subtype of the H5N1 subtype in both Beringkit Markets is 3.3% and Galiran Market is 6.7%, with seroprevalence in the two markets of 5.0% which is statistically not dissimilar (P<0.05). To prevent the transmissions of AI disease at the Beringkit Market and Galiran is recommended for vaccination and more attention to the market management and the free-range chicken maintenance system.

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“…Sel darah merah atau red blood cell disiapkan seperti dijelaskan oleh Darmawi et al (2012), dimana darah diambil dari vena axilaris bawah sayap ayam yang sehat. Darah dimasukkan ke dalam tabung Erlenmeyer bersama gelas mutiara.…”

Section: Penyiapan Sel Darah Merahunclassified

“…Sebanyak 25 μl suspensi sel darah merah 0,5% ditambahkan ke dalam seluruh sumur, microplate digoyang-goyangkan, dan diinkubasi kembali selama 30 menit. Hasil dibaca jika eritrosit pada sumur kontrol telah mengendap seperti dijelaskan oleh Karaca et al (2005) dan Darmawi et al (2012).…”

Section: Uji Hemaglutination Inhibition (Hi Test)unclassified

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Seroprevalensi Avian influenza H5N1 pada Unggas di Kabupaten Aceh Utara

et al. 2013

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Avian influenza virus H5N1 infections are an important cause of diseases in humans and several animal species, including birds. The present study conducted to investigate the seroprevalence Avian Influenza H5N1 in native birds from 15 sub-districts of North Aceh. This study utilized 1108 serum samples collected from the axilaris vein (left or right) of birds. The standard Hemaglutination Inhibition (HI) assay was conducted at Microbiology Laboratory Faculty of Veterinary Medicine of Syiah Kuala University to determined serum antibody possitive or negative reaction against Avian influenza H5N1. The result showed that seroprevalence Avian influenza H5N1 virus was 4,7 % in North Aceh District. There were nine sub-districts were tested positively by HI test. However, the serum collected from six sub-districts did not react (negative) against Avian influenza H5N1. Based on the results we obtained, a conclusion that natural infection by Avian influenza virus in native birds occured in part of North Aceh District.

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Deteksi Antibodi Serum Terhadap Virus Avian influenza pada Ayam Buras

Cited by 5 publications

References 3 publications

Seroprevalence and detection of H5N1 avian influenza virus in local chickens in Tabanan Regency, Bali, Indonesia

Seroprevalence and detection of H5N1 avian influenza virus in local chickens in Tabanan Regency, Bali, Indonesia

Seroprevalensi Penyakit Avian Influenza Subtipe H5n1 Pada Ayam Buras Di Pasar Beringkit Dan Galiran, Bali

Seroprevalensi Avian influenza H5N1 pada Unggas di Kabupaten Aceh Utara

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