The purpose of this study was to investigate the toxicity and the distribution of mercury (Hg) in the main tissues of freshwater fish (Oreochromis niloticus) after being exposed to water containing Hg(II). A sample group of 10 fish, of mean weight 80–100 g wet weight, were exposed to different concentrations of Hg (0.0012; 0.0049; 0.0141; 0.0524; 0.1126; and 0.5110 mg-HgII/L) for 72 hours under controlled conditions using the static method in ponds. A control medium was also prepared in two replications. Mortality of fish was closely monitored, and the test was repeated three times. For the toxicity test, observations were based on behavior, mortality, and anatomical pathology. The methodology was based on the OECD guidelines for testing of chemicals and lethal concentration (LC50) and particularly using the probit method. Thus, the mean value was obtained from two replications and then further calculated by a software (MiniTab® 16 version). Prior to analysis, samples were first lyophilized. The total concentration of Hg accumulation in the fish organs was analyzed using heat-vaporization atomic absorption spectrometry (HV-AAS) and a MA2000 automatic mercury analyzer. Results showed that toxicity (LC50) of freshwater fish was 0.1435 mg-Hg(II)/L. The internal organs showed some pathological changes including pale gills, anemic eyes, and a whitish body color after the exposure. Furthermore, histopathologically, exposure to mercury might also affect other organs, such as gills, liver, and hepatopancreas. Mercury was found in trace amounts, and its accumulation was found to be at least in the gills. Meanwhile, the highest accumulation was found in the muscle tissue with approximately 5.7183 µg/g dry weight. If they are put in order, the mercury accumulation in the tissue organs was varied from the highest to lowest one: Muscle > eye > bone > head > gill. Finally, it can be concluded that the Hg exposure could affect the histopathological condition of the tested fish.
The aim of this research was to investigate the effect of red watermelon (Citrullus vulgaris) extract to the value of erythrocytes, hemoglobin, and hematocrit of mice (Mus muscullus) exposed to cigarette smoke. This study followed direct complete randomized design by using 12 males’ mice which divided into 4 groups. The treatment group is divided by the negative control group which given 0,5 ml of aquadest, the positive control group which exposed to cigarette smoke and 0,5 ml of aquadest. The treatment group I was exposed to cigarette smoke and given red watermelon extract dose 22 mg/Bw mice. The treatment group II was exposed to cigarette smoke and given red with watermelon extract dose 44 mg/Bw mice. The exposure to cigarette smoke and the given of red watermelon extract were conducted for 30 days. The blood taking was performed on day 31 in the Plexus retroorbital. Furthermore, the number of erythrocytes, hemoglobin and hematocrit were calculated. The data were analyzed using ANOVA completely randomized design with SPSS for Windows 16.0. The results of this study showed that the extract of red watermelon for 30 days showed a highly significant effect (P 0.01) to the number of erythrocytes, hemoglobin, and hematocrit of mice. It is concluded that free radicals contained in the cigarette can cause a hemoglobin desaturation, increased blood viscosity, oxidative stress and red watermelon extract can minimize the damage.
ABSTRAK. Penelitian dilakukan untuk mengetahui pengaruh pemanfaatan kulit nanas dalam pakan fermentasi terhadap persentase karkas dan kolesterol daging ayam potong. Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan ternak percobaan sebanyak 80 ekor anak ayam umur 2 hari selama tiga puluh lima hari yang diacak dan dibagi menjadi empat perlakuan dan empat ulangan. Adapun perlakuan ransum disusun berdasarkan tingkat penggunaan kulit nanas yang difermentasi, yaitu: P1 (0,0%), P2 (10%), P3 (20%), dan P4 (30%). Parameter yang diamati adalah persentase karkas dan kolesterol daging ayam potong. Data yang diperoleh dianalisis dengan sidik ragam menggunakan rancangan acak lengkap dan bila terdapat perbedaan dilanjutkan dengan uji Duncan’s Multiple Range Test (DMRT). Hasil penelitian menunjukkan bahwa pakan yang mengandung kulit nanas difermentasi dalam pakan komersil berpengaruh nyata menurunkan (P0,05) persentase karkas dan menurunkan kadar kolesterol pada ayam potong. Dari hasil penelitian disimpulkan bahwa kulit nanas yang difermentasi dalam pakan komersil pada ayam potong mampu menurunkan persentase karkas dan kolesterol pada daging dada ayam potong. (Effect of green leather generation (Ananas comosus L. Merr) fermentation on the percentage of carcass and cholesterol chicken pieces) ABSTRACT. The study was conducted to determine the effect of pineapple skin utilization in fermented feed on carcass and cholesterol percentage of chicken meat. The design used is Completely Randomized Design (RAL) with experimental as many as 80 day old chicken (DOC) aged 2 days, randomly selected, divided into four treatments and four replications and treated for thirty five days. The ration treatment was prepared based on the level of fermented pineapple skin use, namely: P1 (0,0%), P2 (10%), P3 (20%), and P4 (30%). The parameters observed were the percentage of carcass and cholesterol of chicken meat. The data obtained were analyzed by using analysis of variance (ANOVA) and if the were differences followed by Duncan's Multiple Range Test (DMRT) test. The results showed that feed containing fermented pineapple skin significantly decreased (P 0,05) percentage of carcass and lower cholesterol levels in chicken meat. It can be concluded that the utilization of fermented pineapple skin in commercial feed have a significant effect on reducing the percentage of carcass and cholesterol in chicken breast meat.
Malacca (Phyllanthus emblica) is one of the plants that is often by the community in the Aceh Besar district of Indonesia as a traditional medicine for the treatment of various diseases such as antimicrobial, antibacterial, antifungals, antivirals, antimutagenic, antimalaria, and antiallergic. This research was conducted to analyze the content of chemical compounds in the ethanol extract of the Malacca leaf (EEDM) using a gas chromatography-mass spectrophotometer (GC-MS). Malacca leaves were extracted by the maceration method using n-hexane, ethyl acetate, and ethanol. The GC-MS analysis showed EEDM contained 22 chemical compounds. The highest chemical content of EEDM is octadecanoic acid reaching 22.93%, 9,12-octadecanoic acid 14.99%, octadecanoic acid 7.59%, 9-hexadecenoic acid 6.17%, octadecanoic acid 5.95%, octadecanal 5.59%, 9,12-octadecanoic acid 5.06%, 3-eicosyne 4.75%, 1-hexadecenoic acid 4.08%, 11-tetradecen-1-ol 2.92%, 2-furanmethanol 2.83%, delta-guaiene 2.43%, cyclohexane 2.13%, hexadecanoic acid 1.99%, sativen 1.87%, octadecanoic acid 1.52%, 1H-cyclopropaanaphthalene 1.40%, tetradecanoic acid 1.40%, 3,7,11-tridecatrienenitrile 1.20%, caryophellene 1.11%, 2H-pyran 1.07%, and trans-caryophellene 1.03%. This study clearly shows the presence of fatty acids which play a major role in the efficacy of these traditional medicines particularly as antioxidant and antimalarial.
The purpose of this research was to know the motility of Ascaridia galli adult worms in aqueous ethanolic extracts of nuts Veitchia merrillii. The ethanolic extract of the V. merrillii was analyzed. Amount of sixteen head A. galli adult worms were divided into four groups. The first group, worms were left as un-treated normal controls. The second group, worms were treated with concentrations of 0,6 mg/ml levamisole. The third and fourth group, worms were treated with crude aqueous ethanolic extract of 50 and 100 mg/ml concentrations nuts of the V. merrillii, respectively. Motility of A. galli were determined after 12, 24, 36 hour by mean of persentage scored using the following criteria: 3 (moving whole body), 2 (moving only parts of the body), 1 (immobile but alive), and 0 (died). The result of phytochemical V. merrillii contains alkaloids, saponins, flavonoids, tannins, and terpenoids. V. merrillii nuts extract concentrations of 100 mg/ml in vitro can shorten the time motility A. galli adult worms for 12 hours. The study indicated the potential for developing herbal-based anthelmintics to control A. galli.
ABSTRAKPenelitian ini bertujuan mengetahui pengaruh pemberian kombinasi ekstrak kulit batang jaloh 1000 mg/l dalam air minum dan serbuk daun singkong 15% dalam pakan terhadap bobot badan ayam broiler. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan jumlah sampel 20 ekor ayam broiler. Ayam-ayam tersebut kemudian dibagi ke dalam empat kelompok perlakuan, dengan lima ulangan. Perlakuan P0 sebagai kontrol diberikan pakan komersil, P1 (pakan komersil + serbuk daun singkong), P2 (pakan komersil + ekstrak kulit batang jaloh), dan P3 (pakan komersil + ekstrak kulit batang jaloh dan serbuk daun singkong). Data dianalisis dengan analisis varian dan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa P2 berbeda sangat nyata (P<0,01) dengan P0 dan P3, serta berbeda nyata (P<0,05) dengan P1. Kelompok P1 berbeda nyata (P<0,05) dengan P3, dan P3 tidak berbeda nyata (P>0,05) dengan P0 terhadap pertambahan bobot badan, sedangkan pada konsumsi pakan hampir sama antar perlakuan, dan terdapat perbedaan antar perlakuan pada konversi pakan.
The purpose of this study was to characterize the bacterial 16S rRNA gene of Sumatran orangutan (Pongo abelii) Bukittinggi West Sumatera zoo. The sample used in this study are lactic acid bacteria of the Sumatran orangutan (Pongo abelii) derived from zoo Bukittinggi West Sumatra Indonesia. This study was an exploratory study that conducted at the Laboratory through several stages. The first stage was the isolation of LAB from faeces of Sumatran orangutans using MRS agar medium and then cultured in a liquid medium NB. The next stage was the isolation of total DNA, and then, the third stage was the amplification of the 16S rRNA gene and agarose gel electrophoresis. Then,in the fourth stage of determining the DNA sequence and analysis of DNA sequence homology. The final stage was the computational analysis of 16S rRNA gene Sumatran orangutan (Pongo abelii). The results showed that lactic acid bacteria (LAB) from the Sumatran orangutan (Pongo abelii) is close to lactic acid bacteria Lactobacillus helveticus strain IMAU50151 with the levelof similarity of 89%. It is possible that these bacteria is a new species or the species that LAB has not been reported in Genbank.
Pain is a symptom of infection in the body but becomes crucial if it is not treated properly. Pain medication could be managed by administering anti-pain medications (analgesics). Currently, not all commercially available analgesic drugs can be used in animals, due to biochemical and metabolic variations between species causing difficulties in dosage determination and drug administration to the patient. This condition affects the drug efficacy which is incompatible with clinical relevance, has a narrow therapeutic index (NTS), and toxic. Wedelia biflora plants contain triterpenoid compounds that have received considerable attention from paramedics and pharmaceuticals, due to its ability as a therapeutic agent for chemopreventive, analgesic-antipyretic, anti-inflammatory and cancer. The purpose of this study was to obtain the basic ingredients of analgesic drugs. The 60 rats aged 3 months with bodyweight ranged 150-200 g were used in this study and divided into 2 experiments. In the first experiment, 30 rats were divided into 5 groups : K1 (control group) were given aquadest, the K2 was given 0.5 mg/ml meloxicam drug, the groups K3, K4, and K5 were given Wedelia biflora leaf extract with dose of 40 mg/kg BW, 50 mg/kg BW, and 60 mg/kg BW, respectively. The observation was carried out on pain response and blood profile of the rats. In the second experiment, the reflex righting of the rats in 5 group treatments were observed after administered with Wedelia biflora leaves extract at dose of 40, 50, 60, 120, and 240 mg/kg BW, respectively. Central pain test was carried out using tail emersion method and hot plate, while righting reflexes were observed starting from, 30, 60, 120, 240 and 360 minutes after giving sernai leaf extract. The results showed that the Wedelia biflora leaves have the ability as analgesic in the rat without altering the blood profile, and also poses a positive effect on reflex righting.
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