Initial investigation of medicinal plants from Lombok has resulted in the collection of 100 plant species predicted to have antimicrobial, including antimalarial, properties according to local medicinal uses. These plants represent 49 families and 80 genera; 23% of the plants tested positively for alkaloids. Among the plants testing positive, five have been selected for further investigation involving structure elucidation and antimicrobial testing on the extracted alkaloids. Initial work on structural elucidation of some of the alkaloids is reported briefly.

The genus Psychotria is well known as a source of alkaloids and has contributed several novel indole-type alkaloids with quite a broad spectrum of bioactivities. This study was focused on identifying the alkaloids from the leaves of P. malayana Jack from Lombok island on the basis of Gas Chromatography-Mass Spectroscopy (GC-MS) data and the sample preparation followed acid-base extraction. The study showed that the major alkaloid contained by the leaves was hodgkinsine and other minor compounds, namely calycanthine, chimonantine, 2-ethyl-6-methylpyrazine, and 3-methyl-1,2,3,4-tetrahydrogamma-carboline. Bioactivities of the compounds were also discussed.

Overall aims of this research was to study the antibacterial activity of Voacanga foetida and then studied the distribution of the alkaloidal lombine produced in the plant, in which the samples were collected from three different regions. The antibacterial activities were assayed using agar well diffusion method. The distribution of the alkaloids in Voacanga foetida, especially lombine was studied on the basis of GC-MS in several temperature programs. The result showed that the leaves and the barks of Voacanga foetida were possessing antibacterial properties. The second result, GC-MS showed that alkaloidal lombine was produced stably in the barks while not in the leaves. The abundance of lombine in the base fraction of the barks was 6.06% (West Lombok), 74.91% (East Lombok), and 2.77% (North Lombok). The outcome will be then used as a platform for further studies investigating total uses of the plant especially in finding new materials for medicine.

Abstrak. Kumbi atau Voacanga foetida (blume) rolfe merupakan tumbuhan dari family Apocynaceae. Pulau Lombok merupakan daerah utama tempat tumbuhnya 'Kumbi' ini. Kumbi memiliki kandungan selulosa yang cukup tinggi. Selulosa merupakan salah satu bahan baku dalam pembuatan bioetanol. Sebelumnya telah dilakukan penelitian mengenai pembuatan bioetanol dari buah Kumbi (Voacanga foetida (blume) rolfe) ini dengan kadar 14% dan % rendemen sebesar 14,793%. Hasil yang didapatkan ini sudah cukup tinggi namun belum memenuhi syarat untuk dijadikan sebagai bahan baku campuran pada bahan bakar. Tujuan dari penelitian ini untuk mengetahui pengaruh suhu fermentasi terhadap rendemen bioetanol dari buah kumbi menggunakan Saccharomyces cerevisiae dan Rhyzopus oryzae. Metode yang digunakan adalah hidrolisis dengan HCl dan fermentasi menggunakan Saccharomyces cerevisiae dan Rhyzopus oryzae. Berdasarkan penelitian yang telah dilakukan, bioetanol dari buah kumbi dapat diproduksi melalui proses hidrolisis dan fermentasi menggunakan Saccharomyces cerevisiae dan Rhyzopus oryzae dengan variasi suhu fermentasi 28°C, 30°C, 32°C, 34°C, 36°C, 38°C dan 40°C, dan destilasi pada suhu 78 o C, menghasilkan rendemen bioetanol kasar pada suhu optimum 36 °C sebesar 66,02% (w/w) menggunakan Saccharomyces cerevisiae sedangkan dengan rasio yang sama menggunakan Rhyzopus oryzae suhu optimum dicapai pada 32°C, menghasilkan rendemen bioetanol kasar sebesar 88,14% (w/w). Kata kunci: Bioetanol, Buah Kumbi, Hidrolisis, FermentasiAbstract. Kumbi or Voacanga foetida (blume) Rolfe is a plant of the family Apocynaceae. Lombok Island is the main area where the growth of Kumbi. Kumbi has a high cellulose content. Cellulose is a raw material in the manufacture of bioethanol. Research for bioethanol production from fruit Kumbi (Voacanga foetida (blume) Rolfe) generates yield of 14.793%. These results are not yet eligible to be used as raw material in the fuel mixture. This study aims to determine the effect of temperature on the yield of bioethanol fermentation of fruit Kumbi using Saccharomyces cerevisiae and Rhyzopus oryzae. The method used is by HCl hydrolysis and fermentation with Saccharomyces cerevisiae and Rhyzopus oryzae. The results showed that ethanol from Kumbi fruit can be produced by the hydrolysis and fermentation using Saccharomyces cerevisiae and Rhyzopus oryzae. Fermentation temperature variation used was 28 °C, 30 °C, 32 °C, 34 °C, 36 °C, 38 °C, 40 °C and distilled at a temperature of 78 C. The yield of bioethanol 66.02% (w/w ) obtained using Saccharomyces cerevisiae at a temperature of 36 °C while the same ratio used Rhyzopus oryzae produce bioethanol yield of 88.14% (w / w) at a temperature of 32 o C.

The aims of this research are to study the production process and the chemical composition of three traditional Sumbawa oils from Benete Village (BT), Batu Dulang Village (BD), and Hutan Sumbawa (HS). A survey was conducted to learn the production process; and the chemical composition of the Sumbawa oils was determined by fractionation method using three solvents with different polarity (methanol, dichloromethane (DCM) and nhexane), followed by GC-MS analysis of each extract. In general, the three Sumbawa Oils were traditionally produced by mixing various species of medical plants into the coconut oil through heating process. Based on GC-MS analysis, the constituents of the Sumbawa oils are mainly fatty acid, fatty acid ester, monoterpene, and sesquiterpene. Three major identical compounds were identified in all three products with different percentages of abundance namely octadecanoic acid/stearic acid, hexadecanoic acid/palmitic acid, and methyl palmitate. Furthermore, there were other major compounds such as linoleic acid, lycopersene, and dodecanoic acid/lauric acid. The non-natural product compounds found in Sumbawa oil extracts and the biological activities of major compounds are discussed in this article.