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A total of 300 milk samples from buffaloes were evaluated for mastitis and incidence of E. coli. Overall, 157 milk samples (52.33%) were positive for mastitis and out of these positive isolates, 27 bacterial isolates (17.19 %) were identified as E. coli. All the 27 isolates of E. coli were tested for in vitro sensitivity towards 15 antibacterial drugs. The highest resistance was attributed towards antibiotic Kanamycin followed by Cefotaxime, Trimethoprim, Cefotaxime + Clavulanic acid, Amoxycillin, Cefixime, Tetracycline, Ampicillin + Salbactum, Amikacin, Chloramphenicol, Nitrofurantoin, Gentamicin, Cefoperazone, Ceftriaxone and Ciprofloxacin. Simultaneously resistance to 2 to 9 antibacterial agents, was observed in all 27 (100%) isolates.

Mastitis is an inflammation of mammary glands that is prevalent in dairy bovines. It causes a significant proportion of economic losses to the dairy farmers in India. Cattle and buffalo farming contribute significantly to the economy of the state. Various infectious agents such as bacteria, fungi, and algae may cause mastitis. Hence, it is essential to understand the etiological agents and predisposing factors that lead to mastitis in susceptible bovine populations in Madhya Pradesh state so that appropriate prevention and control strategies can be implemented. In this chapter, epidemiology, diagnosis, prevention, and control measures of mastitis in general and in India, the state of Madhya Pradesh, in particular, will be presented.

Background: Canine parvovirus-2 causes inflammation of the gastrointestinal tract in dogs. Dogs from Mhow and Indore area suffering from symptoms related to gastroenteritis were investigated for the presence of Canine parvovirus-2 infection. Methods: A total of 50 faecal samples from dogs (33 male and 17 female) were collected individually in 5 ml of phosphate buffer saline solution in sterile containers. These samples were tested in haemagglutination assay using pig red blood cells for the presence of Canine parvovirus-2 specific antigen and in a molecular test, polymerase chain reaction, using primers to amplify Canine parvovirus-2 specific product of 681 base pairs. Result: A total of 5 faecal samples (10%) tested positive in haemagglutination assay indicative of presence of Canine parvoviral-2 antigen in the faecal material. Haemagglutinating titre for positive samples ranged from 32 to 1024. Only one faecal sample found positive in polymerase chain reaction test for amplification of Canine parvovirus-2 specific product. The results of the present study indicate presence of Canine parvoviral-2 infection in pet dogs suffering from gastroenteritis, however at a low level.

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Background: Paratuberculosis or Johne’s disease in domestic livestock population is caused by the bacteria, Mycobacterium avium subspecies paratuberculosis. The disease in cattle is characterized by chronic granulomatous enteritis leading to diarrhoea (intermittent or continuous) followed by weakness and emaciation in the affected animals. This research aimed to test cattle population, serologically, for detecting level of Mycobacterium avium subspecies paratuberculosis infection. Methods: In the present study, a total of 180 serum samples from individual cattle of Indore district in Madhya Pradesh tested in a serological test, indigenous enzyme linked immunosorbent assay for detection of antibodies against Mycobacterium avium subspecies paratuberculosis. Result: A total of 49 cattle (27.2%) were found positive in the test for the bio-presence of Mycobacterium avium subspecies paratuberculosis infection.