Antibodies against Mycobacterium avium subspecies paratuberculosis in Cattle of Indore District in Madhya Pradesh

Audarya, S.D.; Singh, Mamta; Chaubey, Kundan Kumar; Gupta, Saurabh; Sikrodia, Ravi; Chhabra, Daljeet; Gangil, Rakhi; Matoli, S.; Sharda, Rakesh; Singh, S.V.

doi:10.18805/ijar.b-4773

Cited by 2 publications

(2 citation statements)

References 21 publications

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“…This opens up few latest possibilities for serological testing to be utilized to detect hardy animals during the initial phases of sickness. In a study, a indigenously developed ELISA was employed to screen serum samples from cattle, evaluating the antibody response to MAP infection and the results indicated a 27.22% prevalence of MAP infection in the cattle population in the Madhya Pradesh region (Audarya et al, 2022).…”

Section: Antibodies (Ab)mentioning

confidence: 99%

Mycobacterium avium Subspecies paratuberculosis Infection and Immunopathological Changes in Animals: A Review

Kumar,

Chaubey,

Dayal

et al. 2024

IJAR

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Mycobacterium avium subspecies paratuberculosis (MAP) is a chronic granulomatous intestinal disease and causative agent of Johne’s disease (JD) in domestic and wild ruminants. This study explores the intricate dynamics of the host immune response in MAP infection and its implications for disease progression and control. Our investigation revealed the pivotal role of interleukin-10 (IL-10) in mediating the communication between the host’s innate and adaptive immunological responses. IL-10 inhibits interferon-gamma (IFN-γ) secretion, altering the balance between Th1 and Th2 responses. In the early stages, a robust Th1 response marked by increased IFN-γ production is observed, but as the disease progresses, a shift towards a humoral Th2 response occurs. Furthermore, we found that IL-10 exerts suppressive effects on macrophage (Mφ) anti-mycobacterial action, leading to increased intracellular survival of mycobacteria. IL-10 also hampers Mφ antimicrobial activity by suppressing the production of essential cytokines such as IL-12 and tumor necrosis factor alpha (TNF-α), crucial for activating natural killer (NK) cells and inducing differentiation of CD4+ T lymphocytes into Th1 effector cells. Importantly, our study sheds light on the intricate interplay between past infections or concurrent active co-infections and their influence on the immune response to unrelated pathogens in the context of paratuberculosis. This review underscores the multifaceted role of IL-10 in the immunopathological manifestation of MAP infection and host immunomodulation. These findings provide valuable insights for both animal and human research in the fight against MAP infection, emphasizing the importance of balancing immune responses and considering the influence of concurrent infections in disease progression.

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Section: Antibodies (Ab)mentioning

confidence: 99%

Mycobacterium avium Subspecies paratuberculosis Infection and Immunopathological Changes in Animals: A Review

Kumar,

Chaubey,

Dayal

et al. 2024

IJAR

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“…Substantial evidence is now available which suggesting that MAP may cause Crohn's disease in humans (Singh et al, 2014a). Among different biotypes of MAP, 'Indian-Bison Type' is the most predominant biotype in India; and it has been detected in broad ruminants species (wild and domestic), other animals like rabbits, primates and human beings (Singh et al, 2014a;2014b;Chaubey et al, 2016;2017;Biswal et al, 2020;Audarya et al, 2022). Based on multiple tests (ZN-staining, i_ELISA, culture and IS900 PCR etc.…”

Section: Introductionmentioning

confidence: 99%

Recombinant Proteins based ‘rELISA’ Field Validation for Differential Diagnosis of Johne’s Disease in Goats

Chaubey,

Singh,

Singh

et al. 2024

AJDFR

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Background: Johne’s disease (JD), caused by Mycobacterium avium subspecies paratuberculosis (MAP), is spreaded worldwide in domestic livestock. It is very clear that JD control program having tedious complications so, only ‘Test and cull’ and ‘vaccination’ strategies are key components in JD control program. ‘Test and cull’ policy is not feasible in Indian condition due to religious beliefs However, vaccination interferes with the immunological tests used for screening of infected and vaccinated (Healthy) animals. Thus, a test that can differentiate between infected and vaccinated animals (DIVA) is needed before implementation of vaccination program. Methods: Herein, we evaluated two in-house ELISA tests i.e., recombinant proteins-based ELISA (rELISA); developed by using six MAP specific recombinant culture filtrate proteins and indigenous ELISA (i_ELISA); developed using whole-cell protoplasmic MAP antigens for their potential. Sera collected from goats under ‘infected’, ‘healthy and vaccinated’ and ‘healthy and non-vaccinated’ groups were tested by both the tests. Result: On analyzing the anti-MAP antibodies level detected by both the ELISAs and anti-MAP antibodies against secretary proteins only detected in i_ELISA, it was concluded that rELISA can differentiate healthy, vaccinated and infected goats, if used in-combination with i_ELISA. Therefore, recombinant proteins based rELISA has potential to be considered as companion tool for future diagnostic for successful implementation of JD vaccination control program.

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Antibodies against Mycobacterium avium subspecies paratuberculosis in Cattle of Indore District in Madhya Pradesh

Cited by 2 publications

References 21 publications

Mycobacterium avium Subspecies paratuberculosis Infection and Immunopathological Changes in Animals: A Review

Mycobacterium avium Subspecies paratuberculosis Infection and Immunopathological Changes in Animals: A Review

Recombinant Proteins based ‘rELISA’ Field Validation for Differential Diagnosis of Johne’s Disease in Goats

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