Molecular Diagnosis of a Cutaneous Form of Pox in Pigeons at Mhow in Madhya Pradesh

Audarya, S.D.; Riyesh, Thachamvally; Kumar, Naveen; Chhabra, Deepak; Sikrodia, Ravi; Sharda, Rohit; Barua, Shyamanta; Garg, U. K.

doi:10.20546/ijcmas.2018.709.157

Cited by 6 publications

(9 citation statements)

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“…The finding of present study supports the findings of Kabir et al (2015), Audarya et al (2018), Masola et al(2014) and Roy et al . (2013), who mentioned thickening of CAM upon inoculation with FPV and PPV.…”

Section: Propagation and Pcr Detection Of Fpv And Ppv In Embryonated supporting

confidence: 93%

“…Kabir et al(2015)and other researchers who worked on FPV and PPV reported pock lesions on CAM of chicken embryo inoculated with Fowl pox virus and Pigeon pox virus. But in the present study pock lesion was absent on CAM which is similar with the results of Audarya et al (2018), who also reported thickening of the CAM.…”

Section: Propagation and Pcr Detection Of Fpv And Ppv In Embryonated supporting

confidence: 92%

“…Since several passages in unnatural host help to attenuate the virus, for attenuation of FPV and PPV six passages were given in duck embryo for attenuation. Here the infected CAM were thickened and hemorrhagic which supports the findings of Kabir et al (2015), Audarya et al (2018) and Masola et al (2014) that they also reported thickening of CAM.…”

Section: Serial Passage For Attenuation Of the Fpv And Ppv In Duck Emsupporting

confidence: 89%

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Isolation and molecular detection of Fowl pox and Pigeon pox viruses for the development of live attenuated vaccine seeds from the local isolates

Sultana

Nazir

Rahman

et al. 2019

J Bangladesh Agric Univ

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Avipox is a viral disease of fowl and pigeon which is characterized by proliferative and nodular lesions in the feather-free parts of the skin or fibro-necrotic and proliferating part in the mouth, esophagus, and mucous membrane of the upper respiratory tract. This investigation was carried out with an aim to isolate and molecular detection of Fowl pox virus (FPV) and Pigeon pox virus (PPV) for development of live attenuated vaccine seeds from the local virus isolates. In this study, nodular lesions were collected from seven pigeons and four chickens from different areas of Mymensingh in Bangladesh which were affected by pox. Viral inoculums were prepared and DNA materials were extracted for PCR-based identification of P4b genes. Detection of virus was confirmed by PCR following propagation into 9-11 days old embryonated chicken egg (ECE) and also chicken embryo fibroblast (CEF) cell culture All the field samples were found positive for FPV and PPV by PCRR. These field isolates were propagated and attenuated in duck embryo through CAM route and duck embryo fibroblast (DEF) cell culture for the development of live attenuated vaccine seeds. Attenuation of both FPV and PPV were successful in duck embryo through CAM route and duck embryo fibroblast (DEF) cell culture after serial six passages. Attenuation of the virus was confirmed by inoculation into experimental birds. Inoculation of attenuated FPV and PPV in chicken and pigeon respectively exhibited no pox lesions whereas control chicken and pigeon inoculated with field isolates develop nodular lesions. Both FPV and PPV were confirmed from both groups of birds by PCR. These attenuated local isolates of FPV and PPV could be used as potential vaccine candidates for the prevention and control of fowl pox and pigeon pox in Bangladesh. J. Bangladesh Agril. Univ. 17(2): 211–219, June 2019

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Section: Propagation and Pcr Detection Of Fpv And Ppv In Embryonated supporting

confidence: 93%

Section: Propagation and Pcr Detection Of Fpv And Ppv In Embryonated supporting

confidence: 92%

Section: Serial Passage For Attenuation Of the Fpv And Ppv In Duck Emsupporting

confidence: 89%

See 1 more Smart Citation

Isolation and molecular detection of Fowl pox and Pigeon pox viruses for the development of live attenuated vaccine seeds from the local isolates

Sultana

Nazir

Rahman

et al. 2019

J Bangladesh Agric Univ

View full text Add to dashboard Cite

show abstract

“…In addition, in the current study, there was cytopathic effect on fibroblast embryonated chicken eggs which characterized with rounding, aggregation and detachment of cells from monolayer which showed after 24h and 48h of 1 st and 2 nd passage respectively, which is consistent with the results of (Ghalyanchilangeroudi et al, 2018;Audarya et al, 2018) revealed that the virus had the same impact on these kinds of cells. Sultana et al (2019) showed the appearance of cytopathic effect which include rounding, necrotic lesions, degeneration and clumping of cells beside the formation of giant cell that observed due to fowl poxvirus and pigeon poxvirus at chicken embryos fibroblast.…”

Section: Phylogenetic Analysis P4b Gene For Pigeon Poxvirussupporting

confidence: 89%

“…The phylogenic analysis of the P4b gene of Iraqi pigeon poxvirus, which was deposited in NCBI under the accession number and studied in comparison to other registered types regarding the P4b gene, revealed that the Iraqi PPV was 99 percent homologous to the Egypt strain (MN892361.1), as shown in Table 2 and Figure The current study's findings support the findings of (Bayati, 2017;Ghalyanchilangeroudi et al, 2018;Audarya et al, 2018;Sultana et al, 2019), who reported CAM thickening after PPV inoculation and development of Pock lesions. Bayati (2017) demonstrated that these isolates produced pocks lesions at the 3 rd and 4 th passages, whereas thickening and necrosis of CAM were only seen at the first passage.…”

Section: Phylogenetic Analysis P4b Gene For Pigeon Poxvirussupporting

confidence: 84%