Isolation of E. coli from Bovine Mastitis and Their Antibiotic  Sensitivity Pattern

Singh, Ayushi; Chhabra, Daljeet; Sikrodia, Ravi; Shukla, Susmita; Sharda, Rakesh; Audarya, S.D.

doi:10.20546/ijcmas.2018.710.002

Cited by 11 publications

(9 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Regarding the antibiotic susceptibility test of E. coli and S. aureus, the result showed an emerging antibiotic resistance among both isolates. All the E. coli isolates in this study were resistant to Ampicillin which was in compliance with the findings of Badri et al (2017) [9] and Singh et al (2018) [25]. Besides ampicillin resistance, the results revealed higher resistance among E. coli towards Nalidixic acid, Tetracycline, Ceftriaxone and Ciprofloxacin in descending order.…”

Section: Discussionsupporting

confidence: 89%

Antibiogram of Escherichia coli and Staphylococcus aureus isolated from milk sold in Kathmandu district

et al. 2020

View full text Add to dashboard Cite

The emergence of antibiotic resistance in microorganisms and the presence of such isolates in milk pose a great risk to public health. Therefore, this study aims to determine the antibiotic susceptibility pattern of Escherichia coli and Staphylococcus aureus isolated from milk and assess the microbial quality of milk. For this, a total of 70 milk samples were collected and the total bacterial count (TBC) was determined. E. coli and S. aureus were isolated using their respective selective media while antibiotic susceptibility testing was carried out by Kirby Bauer Disc Diffusion method. The TBC showed that the raw milk samples contained two-fold higher microbial load while the pasteurized milk samples contained four-fold higher microbial loads than the standard guidelines. A total of 62 isolates were identified from culture-positive milk samples of which 32 were E. coli and 30 were S. aureus. A significant correlation was observed between microbial load and the organism isolated (r = 0.339, p<0.01). All S. aureus isolates were susceptible to Chloramphenicol while 40% were resistant to Cefoxitin, indicating the presence of Methicillin resistant S. aureus (MRSA). Also, 12 multidrug resistant (MDR) S. aureus were identified. While for E. coli, all were susceptible to Chloramphenicol but resistant to Ampicillin. Also, 9 MDR E. coli were detected. Higher resistance was observed among isolates from the raw milk samples than the pasteurized milk. It can be concluded that the milk produced by small-scale farms and dairy industries of Kathmandu district are of poor quality. Hence, routine microbial quality assessment and antimicrobial resistance monitoring should be followed to safeguard public health.

show abstract

Section: Discussionsupporting

confidence: 89%

Antibiogram of Escherichia coli and Staphylococcus aureus isolated from milk sold in Kathmandu district

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Generally, 33 (12.10%) milk samples were displayed positive results for E. coli . Similar findings were recorded by Singh et al (2018) who observed that 27 (17.19%) E. coli strains were recovered from 157 milk samples of buffalo mastitis. Several previous studies were largely similar to the current results, and the percentage of E. coli from the milk of buffaloes infected with mastitis ranged between 15 and 18% ( Ali et al, 2011 , Bhanot et al, 2012 , El-Sayed Lamey et al, 2013 ).…”

Section: Discussionsupporting

confidence: 88%

Multidrug-resistant Escherichia coli in Raw Milk: Molecular Characterization and the potential impact of camel’s Urine as an Antibacterial Agent

Elbehiry

Marzouk

Moussa

et al. 2021

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

Raw milk is one of the most important vehicles for transmitting various pathogens, especially Escherichia coli ( E. coli ). Multidrug-resistant pathogens are highly prevalent among mastitic cows in various dairy farms worldwide. Therefore, our current study is based on the identification of E. coli from mastitic cow’s milk and their resistance to various antibacterial agents. As well, the impact of camel’s urine on multi-drug resistant E. coli were also evaluated. Thirty-three E. coli isolates were recovered from 254 milk samples. All strains were initially identified phenotypically by culturing on specific media and Vitek 2 Compact System. The protein fingerprinting technique was used as a confirmatory method. The Stx1 , Stx2 and eae genes were also verified by polymerase chain reaction (PCR). The antimicrobial resistance of E. coli strains was tested by the Vitek 2 AST-GN69 cards. Thirty multi-drug resistant E. coli strains (20 from mastitic milk and 10 from clinical samples) were laboratory tested with different concentrations (100%, 75%, 50% and 25%) of virgin and breeding camel’s urine, using the paper disc diffusion method. Our findings showed that 93.94% of E. coli strains were recognized by the Vitek™ 2 system. The results of proteomic investigation illustrated that 100% of E. coli strains were identified at log values ≥2.00. The genotypic identification of the three virulence genes illustrated that 90.1%, 63.64%, and 30.55% of E. coli strains were able to carry the Stx1 , eae, and Stx2 genes, respectively. Most strains of E. coli showed strong resistance against cefazolin (78.79%), ceftazidime (66.67%), cefotaxime (60.61%), ceftriaxone (54.55%), and cefepime (39.40%). The results of the antibacterial effect of camel’s urine revealed that the mean inhibitory zones of virgin camel’s urine were 28 mm, 17 mm, and 14 mm, for the concentrations of 100%, 75%, and 50%, respectively. Whereas; the inhibitory zones for the breeding camel’s urine were 18 mm, 0 mm, and 0 mm, for the concentrations of 100%, 75%, and 50%, respectively. We concluded that the majority of E. coli strains were able to harbor some virulence genes and resist many antibiotics. Our study also provided a robust evidence that the camel’s urine, particularly from the virgin camels has robust antimicrobial activity against multidrug-resistant E. coli strains.

show abstract

“…Incidence of streptococci (32.53%), micrococci (5.74%), Corynebacterium (1.91%), and Escherichia coli (0.95%) was reported in cases of bovine mastitis. However, recently, 17.19% mastitis cases in buffalo were due to infection with Escherichia coli [27][28][29][30][31].…”

Section: Etiology Of Mastitismentioning

confidence: 99%

Epidemiology of Bovine Mastitis and Its Diagnosis, Prevention, and Control

Audarya¹,

Chhabra²,

Sharda³

et al. 2022

Mastitis in Dairy Cattle, Sheep and Goats

Self Cite

View full text Add to dashboard Cite

Mastitis is an inflammation of mammary glands that is prevalent in dairy bovines. It causes a significant proportion of economic losses to the dairy farmers in India. Cattle and buffalo farming contribute significantly to the economy of the state. Various infectious agents such as bacteria, fungi, and algae may cause mastitis. Hence, it is essential to understand the etiological agents and predisposing factors that lead to mastitis in susceptible bovine populations in Madhya Pradesh state so that appropriate prevention and control strategies can be implemented. In this chapter, epidemiology, diagnosis, prevention, and control measures of mastitis in general and in India, the state of Madhya Pradesh, in particular, will be presented.

show abstract

Isolation of E. coli from Bovine Mastitis and Their Antibiotic Sensitivity Pattern

Cited by 11 publications

References 10 publications

Antibiogram of Escherichia coli and Staphylococcus aureus isolated from milk sold in Kathmandu district

Antibiogram of Escherichia coli and Staphylococcus aureus isolated from milk sold in Kathmandu district

Multidrug-resistant Escherichia coli in Raw Milk: Molecular Characterization and the potential impact of camel’s Urine as an Antibacterial Agent

Epidemiology of Bovine Mastitis and Its Diagnosis, Prevention, and Control

Contact Info

Product

Resources

About