Summary Introduction: There is a high interest in creating medicines, dietary supplements, cosmetics including plant extract with antioxidant activity. For understanding whether plant extract has a maximum level of antioxidant activity it is important to know the total antioxidant activity of raw material. Objective: The main goal of study was to find out the green tea leaves total antioxidant activity. Methods: The antioxidant activity was measured by potentiometric method. Total phenolic, flavonoids, catechins and hydrocinnamic acids derivatives were quantified using Folin-Ciocalteu, aluminium chloride, vanillin and sodium molibdate methods, respectively. Results: The green tea leaves total antioxidant activity was 660.75 mmol-eqv./mres. dry weight. A significant correlation was observed between the amount of phytochemicals and antioxidant activity, which indicated its main role in antioxidant activity. Conclusion: The research showed that the green rea leaves possess a high value of antioxidant activity and it is a good source of phenolic constituents.
Aim. To develop the composition and technology for obtaining a dietary supplement “Cachinol” with the antioxidant activity in the form of granules used in the polycystic ovary syndrome. Materials and methods. To achieve the goal, physical, physicochemical, pharmacotechnological and statistical research methods, as well as generally accepted research methods of the State Pharmacopoeia of Ukraine (SPhU) 2.0 were used in the work. Results and discussion. The rational dosage form is granules. The green tea leaf extract and myo-inositol were chosen as the active pharmaceutical ingredients of granules. For 3 batches of granules, the following parameters were determined: the fractional composition ranging from 13.2 to 17.9 % for 3-2 mm, from 45.3 to 56.2 % for 2-1 mm, from 20.1 to 26.1 % for 1-0.5 mm, from 8.1-12.4 for 0.5-0.25 mm, from 2.95-4.6 % for < 0.25 mm; the moisture content of granules ranging from 2.80-3.10 %; the bulk density – from 0.49 to 0.52 g/cm3, the tapped density – from 0.54 to 0.58 g/cm3; the flowability was in the range of 8.00-8.25 g/s; the angle of repose – from 30 to 33°; disintegration – from 41 to 45 s. Conclusions. The flowchart for obtaining granules in industrial conditions has been developed. The technological process for obtaining granules consists of 8 technological stages. The studies of the technological parameters of granules have been performed in accordance with the requirements of the SPhU 2.0. It has been found that the granules developed meet the requirements of the SPhU 2.0 and can be recommended for further research.
Much attention is currently paid to the study of the antioxidant properties of various objects – individual antioxidants, dietary supplements, medicines, liquid plant extracts. Antioxidant medicines are widely used as the main or additional correction agents in the treatment of a number of diseases. Therefore, the study and development of procedures for determining the antioxidant activity is a prospective task for today. Aim. To determine the contribution of different concentrations of ethanol to the level of the antioxidant activity (AOA) of ascorbic acid solutions by the potentiometric method. Results and discussion. The different ethanol content in the solution had the following percent of the contribution to the value of AOA of ascorbic acid solutions – 1.85, 3.56, 4.89, 6.76, 7.63 % for 20, 40, 60, 80, 96 % ethanol, respectively. The linearity of the procedure was proven in the range from 0.039 to 0.31 mmol/L. Experimental part. The object of the study was solutions of ascorbic acid prepared using ethanol of different concentrations – 20, 40, 60, 80, 96 %. Potentiometric measurements were conducted by a Hanna 2550 pH meter (Germany) with an EZDO 5010 combined platinum electrode. Weighing was carried out using an АN100 digital analytical balance (AXIS, Ukraine) with d = 0.0001 g. Ascorbic acid was purchased from Sigma Aldrich (≥ 99.0 %), K3[Fe(CN)6], K4[Fe(CN)6], NaHPO4, KH2PO4 were of analytical grade. Conclusions. It has been found that ethyl alcohol affects the change of the potential in the electrochemical cell and the level of AOA of ascorbic acid solutions. The percentage of the contribution of different concentrations of ethyl alcohol to the AOA value ranges from 1.85 to 7.63 %. The approach and the formula for calculation that take into account the effect of ethyl alcohol on the final AOA result of the test sample of ascorbic acid in a water-alcohol solutions have been proposed. The results of this study can be used in the pharmaceutical and food industries to determine, assess and control the AOA level of dietary supplements, liquid extracts, tinctures, medicines, and alcoholic beverages.
The aim of the work is to determine and evaluate the antioxidant activity of dietary supplements with green tea extract, as well as to introduce conditional terms of the level of AOA. Materials and methods. The object of the study was dietary supplements of different manufactures: “Green Tea Extract” of Natural Sources, USA (DS1), “Extract of green tea” of Elit-Pharm, Ukraine (DS2) and “Green tea” of Pharmakom, Ukraine (DS3). Potentiometric measurements were conducted by pH meter Hanna 2550 (Germany) with a combined platinum electrode EZDO 5010. Epigallocatechin-3-O-gallate ˃98.0 % (Sigma Aldrich), K3[Fe(CN)6], K4[Fe(CN)6], NaHPO4, KH2PO4 were analytical grade. Results. It was established that a value of the antioxidant activity of DS1 was 36.51 mmol/tab, DS2 – 29.78 mmol/tab, and DS3 – 16.67 mmol/tab. DS1 had the highest value of the antioxidant activity, which correlated with the content of catechins (r2 = 0.9314). According to the proposed conditional terms of antioxidant activity dietary supplements, DS1 and DS2 possessed a low level of antioxidant activity, whereas DS3 corresponded to very low level of antioxidant activity. Conclusions. It was found that the studied dietary supplements with green tea extract have antioxidant activity, which correlates with the content of catechins.
The aim of the study. To determine phenolic and organic acids compound profiles, and the antioxidant, antibacterial and anti-inflammatory activities of raspberry leaf extract. Materials and methods. The object of the study was red raspberry leaf extract. The quantity of phenolic compounds was determined by applying a spectrophotometric method of analysis, whereas organic acids content was assessed by means of the alkalimetric method, while the antioxidant activity of the obtained extract was evaluated by employing the potentiometric method, and antibacterial and antifungal activity was ascertained through the wells method, and anti-inflammatory activity was found via carrageenan – induced paw edema assay. Results. The content of phenolic compounds was 18.45±0.37 mg/ml, catechins was 10.12±0.20 mg/ml, flavonoids was 3.32±0.07 mg/ml, hydroxycinnamic acids derivatives was 2.39±0.05 mg/ml and organic acids was 7.25±0.15 mg/ml. Moreover, the antioxidant activity was 76.11±1.48 mmol-equiv./mres dry, which was higher by 32.80% than the reference drug „Ascorutin”. Staphylococcus aureus bacteria was the most sensitive to the extract (25.00±0.00 mm), whereas Pseudomonas aeruginosa was the most resistant (21.67 ±0.66 mm). Treatment with red raspberry leaf extract at 1 ml/kg showed a significant edema reduction at 1, 2 and 3 h at 38.8, 41.8 and 48.8%, compared with the saline group. Conclusian. The present study indicated that red raspberry leaf extract possesses antioxidant, antibacterial and anti-inflammatory activities. Thus, red raspberry leaves are a promising source of bioactive substances that can be used for further developing medicines in the treatment and prevention of lifestyle diseases.
Aim. To study the qualitative composition, the quantitative content of catechins in green tea leaves and compare the data obtained with those evaluated by spectrophotometry.Materials and methods. Green tea leaves used for the analysis were collected in Anhui Province, China. The extract for the HPLC analysis was obtained by the maceration method with 60 % ethanol twice in the raw material/extractant ratio of 1 : 20. In the case of the spectrophotometric analysis, green tea leaves were extracted with 70 % ethanol twice by the maceration method in the raw material/extractant ratio of 1 : 20. The analysis of the extract from green tea leaves was performed by high performance liquid chromatography using a Prominence LC-20 Shimadzu chromatographic system (Japan) with a SPD-20AV spectrophotometric detector, an Agilent Technologies Microsorb-MV-150 column (reversed phase, C18 modified silica gel, length – 150 mm, diameter – 4.6 mm, particles size – 5 μm). Substances in the extract were identified by comparing the retention time and the spectral characteristics of the test substances with the same characteristics of the reference standards. Spectrophotometric measurements were carried out using a UV-1000 single beam spectrophotometer (China) with the pair of S90-309Q quartz square cells.Results and discussion. Using high performance liquid chromatography 5 catechins were identified. Among them epigallocatechin-3-O-gallate (10.85 %) predominated, while catechin (0.61 %) had the lowest concentration. The total amount of catechins in green tea leaves was 30.56 and 24.79 % by HPLC and spectrophotometry, respectively. The F- and t-tests showed that there was no significant difference between the results of HPLC and spectrophotometry.Conclusions. The qualitative composition and the quantitative content of catechins have been determined in the extract from green tea leaves by high performance liquid chromatography and spectrophotometry. Both HPLC and spectrophotometric methods can be used to determine the total catechin content in green tea leaves. The high content of catechins makes the extract promising for further study and creation of new herbal medicinal products and dietary supplements. The results obtained will be used for standardization of green tea leaves and for future pharmacological research of its extract.
Recently, there has been a high demand from the population for dietary supplements that have antioxidant activity. First of all, these are dietary supplements with green tea leaf extract, because green tea contains catechins in a fairly high amount. Nowadays, according to the current legislation of Ukraine the qualitative composition and quantitative content of biologically active substances in dietary supplements are not established, which to a certain extent affects the quality and safety of these products. The aim of the study was to determine the qualitative composition and quantitative content of some groups of BAS in dietary supplements with green tea leaf extract. Materials and methods. Three dietary supplements from different manufacturers “Green Tea Extract”, “Green Tea Extract”, “Green Tea” were chosen for the study. Qualitative analysis was performed by thin layer chromatography, for quantification spectrophotometry in the UV-region was used. Results. The presence of catechins was found in the studied dietary supplements, caffeine was found only in trace amounts. The total content of catechins is 146.80 ± 1.36 mg, 79.00 ± 0.88 mg, 28.00 ± 0.75 mg, and in terms of green tea extract, which is stated in dietary supplements for dietary supplements “Green Tea Extract”, “Green Tea Extract”, “Green Tea”, respectively. Conclusions. Qualitative and quantitative analysis of some groups of BAS of three dietary supplements with green tea leaf extract was performed. It was found that dietary supplements “Green Tea Extract” and “Green Tea” meet the requirements of the US Pharmacopoeia 38 in terms of catechins. Based on the study, it can be concluded that the problem of compliance with dietary supplements is relevant today and requires the introduction of regulatory documentation for the detection and determination of biologically active substances in dietary supplements.
Organic acids are a large group of biologically active compounds that perform important functions in the plant organism. Moreover, all plants, regardless of the species and family, contain organic acids to a small or large extent as organic acids belong to intermediate metabolites arising from the oxidation of proteins and amino acids, fats and carbohydrates.Aim. To validate the method of alkalimetry proposed with potentiometric detection of the end-point for the quantitative determination of free organic acids in raspberry leaves.Results and discussion. The method proposed was validated according to the International Conference on Harmonization (ICH) guidelines. The linearity was in the concentration range of 40 – 200 % (r2 = 0.9991). The percentage of recovery was found to be in the range of 98.77 – 102.48 %. The repeatability and intermediate precision were 1.58 % and 1.74 %, respectively. The method is accurate and reliable, with the relative standard deviation of less than 2 %.Experimental part. Leaves of raspberry were collected in the Kharkiv region during the period of full ripening. A Hanna 2550 pH-meter with a HI 1131P potentiometric electrode was used for alkalimetric titration of free organic acids. The titration was carried out using a microburette with Class A accuracy.Conclusions. The alkalimetry method for the quantitative determination of free organic acids in raspberry leaves has been proposed and validated according to the following parameters: specifcity, linearity, accuracy, repeatability, intermediate precision, robustness. It has been confrmed that the method is simple, reliable, accurate and cost-effective.Key words: raspberry; leaves; free organic acids; alkalimetry; validation
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