Validation of the alkalimetry method for the quantitative determination of free organic acids in raspberry leaves

Maslov, O. Yu.; Kolisnyk, S. V.; Костина, Т. А.; Shovkova, Z. V.; Ahmedov, E. Yu.; Komisarenko, M. A.

doi:10.24959/ophcj.21.226278

Cited by 5 publications

(3 citation statements)

References 9 publications

(7 reference statements)

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“…Organic acids are a group of biologically active compounds that hold vitamin properties and a chlorotic effect and enhance the secretion of bile and pancreatic juices [30]. According to recent studies, organic acids also show potent antibacterial activity against Gram-positive and Gram-negative strains [31].…”

Section: Discussionmentioning

confidence: 99%

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Investigation the influence of biologically active compounds on the antioxidant, antibacterial and anti-inflammatory activities of red raspberry (Rubus idaeous l.) leaf extract

Maslov

Komisarenko

Ponomarenko³

et al. 2022

Current Issues in Pharmacy and Medical Sciences

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The aim of the study. To determine phenolic and organic acids compound profiles, and the antioxidant, antibacterial and anti-inflammatory activities of raspberry leaf extract. Materials and methods. The object of the study was red raspberry leaf extract. The quantity of phenolic compounds was determined by applying a spectrophotometric method of analysis, whereas organic acids content was assessed by means of the alkalimetric method, while the antioxidant activity of the obtained extract was evaluated by employing the potentiometric method, and antibacterial and antifungal activity was ascertained through the wells method, and anti-inflammatory activity was found via carrageenan – induced paw edema assay. Results. The content of phenolic compounds was 18.45±0.37 mg/ml, catechins was 10.12±0.20 mg/ml, flavonoids was 3.32±0.07 mg/ml, hydroxycinnamic acids derivatives was 2.39±0.05 mg/ml and organic acids was 7.25±0.15 mg/ml. Moreover, the antioxidant activity was 76.11±1.48 mmol-equiv./mres dry, which was higher by 32.80% than the reference drug „Ascorutin”. Staphylococcus aureus bacteria was the most sensitive to the extract (25.00±0.00 mm), whereas Pseudomonas aeruginosa was the most resistant (21.67 ±0.66 mm). Treatment with red raspberry leaf extract at 1 ml/kg showed a significant edema reduction at 1, 2 and 3 h at 38.8, 41.8 and 48.8%, compared with the saline group. Conclusian. The present study indicated that red raspberry leaf extract possesses antioxidant, antibacterial and anti-inflammatory activities. Thus, red raspberry leaves are a promising source of bioactive substances that can be used for further developing medicines in the treatment and prevention of lifestyle diseases.

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Section: Discussionmentioning

confidence: 99%

“…The total organic acids content was determined by acidbase titration via the fixation of end-point by applying the potentiometric method [14]. The total content of organic acids in the extract, expressed as citric acid, was calculated according to the following equation:…”

Section: Current Issues In Pharmacy and Medical Sciencesmentioning

confidence: 99%

Investigation the influence of biologically active compounds on the antioxidant, antibacterial and anti-inflammatory activities of red raspberry (Rubus idaeous l.) leaf extract

Maslov

Komisarenko

Ponomarenko³

et al. 2022

Current Issues in Pharmacy and Medical Sciences

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“…The resulting green tea leaf extract was taken as a reference standard since green tea leaves contain their gold standardepigallocatechin-3-gallate. This compound, according to multiple studies, has a potent antioxidant effect [34].…”

Section: Antioxidant Activitymentioning

confidence: 99%

Evaluation of Anti-Inflammatory, Antioxidant Activities and Molecular Docking Analysis of Rubus idaeus Leaf Extract

Maslov,

Komisarenko,

Kolisnyk

et al. 2024

Jordan j. pharm. sci.

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The study aimed to identify the most abundant compounds in raspberry leaf extract via HPLC analysis, conduct theoretical and practical assessments of antioxidant and anti-inflammatory activities both in silico, in vitro, and in vivo, and evaluate the correlation between antioxidant and anti-inflammatory activities. Polyphenols were quantified using HPLC; molecular docking was carried out using AutoDockTools 1.5.6; antioxidant activity was ascertained via the potentiometric method; and anti-inflammatory activity was examined based on the carrageenan edema method. The extract was found to be rich in epicatechin (0.417%), (+)-catechin (0.501%), and ellagitannins (0.401%). The free energy of (+)-catechin and epicatechin was -8.40 and -7.20 respectively for the active sites of cyclooxygenase-2 (COX-2), and -6.60 and -7.11 for nicotinamide adenine dinucleotide phosphate oxidase (NADPH oxidase). Notably, the antioxidant activity of the raspberry leaf extract was 1.43%, 1.04%, and 10.62% higher than that of green tea leaf extract for doses of 4.00, 2.00, 0.20 mg/mL, respectively. Treatment with the raspberry leaf extract at a dose of 13.0 mg/kg resulted in a significant decrease in edema after 1, 2, and 3 hours by 38.8%, 41.8%, and 48.8%, respectively, compared to the control group. The study demonstrated a correspondence between experimental and theoretical results in evaluating antioxidant and anti-inflammatory activities. Correlation analysis further substantiated that the anti-inflammatory action is dependent on antioxidant activity.

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Study of total antioxidant capacity of red raspberry (Rubus idaeous L.) shoots

Maslov

Komisarenko

Golik

et al. 2023

Vitae

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BACKGROUND: Today, cardiovascular, oncological, and neurodegenerative diseases are the main causes of death in the world, according to official World Health Organization (WHO) statistics. Antioxidants are used to treat and prevent these diseases. In order to develop optimal technology for obtaining drugs based on plant extracts with antioxidant action, it is necessary to determine the total antioxidant capacity of raspberry shoots.OBJECTIVES: The study aimed to determine the total antioxidant capacity of red raspberry shoots, study the content of biologically active substances (BAS), and the antioxidant activity of red raspberry shoot extracts obtained during subsequent exhaustive extraction.METHODS: The number of phenolic compounds, catechins, flavonoids, and hydroxycinnamic acids was determined by a spectrophotometric analysis method, whereas organic acids were determined by the alkalimetric method in red raspberry shoot extracts; the antioxidant activity of obtained extracts was evaluated by potentiometric method. RESULTS: The total antioxidant capacity of red raspberry shoots was 164.12 mmol-equiv./m dry weight, the sum of the total content of phenolic compounds was 24.40 mg gallic acid (GA)/mL, catechins – 21.36 mg epigallocatechin-3-O-gallate (EGCG)/mL, flavonoids – 0.77 mg rutin (R)/mL, hydroxycinnamic acids derivatives – 2.56 mg chlorogenic acid (ChA)/mL and organic acids – 1.88 mg citric acid (CA)/mL in red raspberry shoot extracts obtained during subsequent exhaustive extraction. The analysis showed that there is a very high positive correlation between antioxidant activity and total phenolic compounds, catechin, flavonoid, hydroxycinnamic acids derivatives, and organic acids content in red raspberry shoot extracts. CONCLUSIONS: Total red raspberry shoots' antioxidant capacity has been determined. The study results can be used to develop optimal technology for obtaining drugs based on the extract of red raspberry shoots, which has an antioxidant effect.

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Validation of the alkalimetry method for the quantitative determination of free organic acids in raspberry leaves

Cited by 5 publications

References 9 publications

Investigation the influence of biologically active compounds on the antioxidant, antibacterial and anti-inflammatory activities of red raspberry (Rubus idaeous l.) leaf extract

Investigation the influence of biologically active compounds on the antioxidant, antibacterial and anti-inflammatory activities of red raspberry (Rubus idaeous l.) leaf extract

Evaluation of Anti-Inflammatory, Antioxidant Activities and Molecular Docking Analysis of Rubus idaeus Leaf Extract

Study of total antioxidant capacity of red raspberry (Rubus idaeous L.) shoots

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