Study of the qualitative composition and quantitative content of some groups of BAS in dietary supplements with green tea leaf extract

Maslov, O. Yu.; Kolisnyk, S. V.; Hrechana, O. V.; Serbin, A. H.

doi:10.14739/2310-1210.2021.1.224932

Cited by 5 publications

(4 citation statements)

References 5 publications

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“…In our previous research [8], the total content of catechins was established by absorption spectrophotometry. It was found that the total content of catechins was 146.80 ± 1.36, 79.0 ± 0.88, 28.0 ± 0.75 mg in DS1, DS2, and DS3, respec tively.…”

Section: Resultsmentioning

confidence: 99%

Study and evaluation antioxidant activity of dietary supplements with green tea extract

Maslov¹,

Kolisnyk²,

Komisarenko³

et al. 2021

АПФМНП

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The aim of the work is to determine and evaluate the antioxidant activity of dietary supplements with green tea extract, as well as to introduce conditional terms of the level of AOA. Materials and methods. The object of the study was dietary supplements of different manufactures: “Green Tea Extract” of Natural Sources, USA (DS1), “Extract of green tea” of Elit-Pharm, Ukraine (DS2) and “Green tea” of Pharmakom, Ukraine (DS3). Potentiometric measurements were conducted by pH meter Hanna 2550 (Germany) with a combined platinum electrode EZDO 5010. Epigallocatechin-3-O-gallate ˃98.0 % (Sigma Aldrich), K3[Fe(CN)6], K4[Fe(CN)6], NaHPO4, KH2PO4 were analytical grade. Results. It was established that a value of the antioxidant activity of DS1 was 36.51 mmol/tab, DS2 – 29.78 mmol/tab, and DS3 – 16.67 mmol/tab. DS1 had the highest value of the antioxidant activity, which correlated with the content of catechins (r2 = 0.9314). According to the proposed conditional terms of antioxidant activity dietary supplements, DS1 and DS2 possessed a low level of antioxidant activity, whereas DS3 corresponded to very low level of antioxidant activity. Conclusions. It was found that the studied dietary supplements with green tea extract have antioxidant activity, which correlates with the content of catechins.

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Section: Resultsmentioning

confidence: 99%

Study and evaluation antioxidant activity of dietary supplements with green tea extract

Maslov¹,

Kolisnyk²,

Komisarenko³

et al. 2021

АПФМНП

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“…The powder of a crushed tablet and the capsule contents of dietary supplements were completely dissolved in 96 % ethanol and filtered into a 25.0 mL volumetric flask, and diluted to the volume with the same solvent. The mobile phase was hydrochloric acid -formic acidn-butanol (16 : 19 : 65) for detection of anthocyanins [16], toluene -acetone -formic acid (9 : 9 : 2) for catechins [17], ethyl acetate -glacial acetic acid -formic acid -water (100 : 11 : 11 : 26) for flavonoids and phenolic acids [18]. The following standard solutions were used: 0.1 % cyaniding-3-glucoside and 0.1 % pelargonidin-3-glucoside (for anthocyanins); 0.25 % epicatechin and 0.1 % epigallocatechin-3-O-gallate (for catechins); 0.25 % rutin, 0.1 % chlorogenic acid, 0.01 % ellagic acid and caffeic acid (for flavonoids and phenolic acids).…”

Section: Tlc Analysis Assaymentioning

confidence: 99%

The study of some quality parameters and the antioxidant activity of dietary supplements with the pomegranate extract (Punica granatum L.)

Maslov,

Komisarenko,

Kolisnyk

et al. 2023

News of pharm.

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Aim. To conduct a qualitative, quantitative analysis and determine the antioxidant activity of dietary supplements with the pomegranate extract. Materials and methods. Three dietary supplements from different manufacturers – “Extract of pomegranate” (manufactured by Source Naturals), “Extract of pomegranate” (manufactured by Puritan Pride), and “Extract of pomegranate” (manufactured by Vitacost) – were chosen for the study. The thin layer chromatography (TLC) was used to perform the qualitative analysis, the quantity of biologically active substances (BAS) in dietary supplements was determined by the spectrophotometric method, the antioxidant activity – by potentiometric method. The qualitative analysis was performed by thin-layer chromatography (TLC); spectrophotometry was used for the quantitative determination, and the potentiometric method was applied to determine the antioxidant activity. Results and discussions. The presence of anthocyanins, flavonoids, catechins and phenolic acids were detected in the dietary supplements analyzed. The total content of phenolic compounds was 201.00 ± 6.03, 159.00 ± 4.77, 110.30 ± 3.30 mg, catechins was 16.10 ± 0.50, 11.60 ± 0.50, 7.33 ± 0.50 mg, anthocyanins was 43 ± 1.29, 32.00 ± 0.96, 22.00 ± 0.66 mg, and flavonoids was 30.15 ± 0.90, 29.44 ± 1.00, 18.21 ± 0.55 mg. The antioxidant activity was 266.11 ± 5.32, 212.12 ± 4.24, 150.81 ± 3.02 mmol-eqv./mextr for dietary supplements “Extract of pomegranate” (manufactured by Source Naturals), “Extract of pomegranate” (manufactured by Puritan Pride), “Extract of pomegranate” (manufactured by Vitacost) in 1 dosage form, respectively. Conclusions. The analysis of the qualitative composition, the quantitative content of BAS and the antioxidant activity of dietary supplements with the pomegranate extract allows us to find out that dietary supplements manufactured by Source Naturals and Puritans Pride meet the requirements of the State Pharmacopoeia of Ukraine (SPhU), while a dietary supplement manufactured by Vitacost does not correspond the requirements. The results of the study allow us to state that the problem of compliance of dietary supplements is relevant; therefore, it is necessary to introduce normative documentation for the identification and determination of BAS in dietary supplements.

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“…Nowadays, analytical methods, such as ion chromatography [11], high performance liquid chromatography-ultraviolet detection (HPLC-UV) [12], capillary electrophoresis [13], photocolorimetry [14], UV-spectrophotometry [15] and thin-layer chromatography [16], are commonly applied to analyze catechins.…”

Section: Introductionmentioning

confidence: 99%

Determination of catechins in green tea leaves by HPLC compared to spectrophotometry

Maslov

Komisarenko

Kolisnyk

et al. 2021

J. org. pharm. chem.

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Aim. To study the qualitative composition, the quantitative content of catechins in green tea leaves and compare the data obtained with those evaluated by spectrophotometry.Materials and methods. Green tea leaves used for the analysis were collected in Anhui Province, China. The extract for the HPLC analysis was obtained by the maceration method with 60 % ethanol twice in the raw material/extractant ratio of 1 : 20. In the case of the spectrophotometric analysis, green tea leaves were extracted with 70 % ethanol twice by the maceration method in the raw material/extractant ratio of 1 : 20. The analysis of the extract from green tea leaves was performed by high performance liquid chromatography using a Prominence LC-20 Shimadzu chromatographic system (Japan) with a SPD-20AV spectrophotometric detector, an Agilent Technologies Microsorb-MV-150 column (reversed phase, C18 modified silica gel, length – 150 mm, diameter – 4.6 mm, particles size – 5 μm). Substances in the extract were identified by comparing the retention time and the spectral characteristics of the test substances with the same characteristics of the reference standards. Spectrophotometric measurements were carried out using a UV-1000 single beam spectrophotometer (China) with the pair of S90-309Q quartz square cells.Results and discussion. Using high performance liquid chromatography 5 catechins were identified. Among them epigallocatechin-3-O-gallate (10.85 %) predominated, while catechin (0.61 %) had the lowest concentration. The total amount of catechins in green tea leaves was 30.56 and 24.79 % by HPLC and spectrophotometry, respectively. The F- and t-tests showed that there was no significant difference between the results of HPLC and spectrophotometry.Conclusions. The qualitative composition and the quantitative content of catechins have been determined in the extract from green tea leaves by high performance liquid chromatography and spectrophotometry. Both HPLC and spectrophotometric methods can be used to determine the total catechin content in green tea leaves. The high content of catechins makes the extract promising for further study and creation of new herbal medicinal products and dietary supplements. The results obtained will be used for standardization of green tea leaves and for future pharmacological research of its extract.

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Study of the qualitative composition and quantitative content of some groups of BAS in dietary supplements with green tea leaf extract

Cited by 5 publications

References 5 publications

Study and evaluation antioxidant activity of dietary supplements with green tea extract

Study and evaluation antioxidant activity of dietary supplements with green tea extract

The study of some quality parameters and the antioxidant activity of dietary supplements with the pomegranate extract (Punica granatum L.)

Determination of catechins in green tea leaves by HPLC compared to spectrophotometry

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