Determination of catechins in green tea leaves  by HPLC compared to spectrophotometry

Maslov, O. Yu.; Komisarenko, M. A.; Kolisnyk, Yulia S.; Костина, Т. А.

doi:10.24959/ophcj.21.238177

Cited by 8 publications

(4 citation statements)

References 16 publications

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“…A potential medicinal plant for averting and managing dental caries is the green tea leaf. In our studies, it was found that green tea leaf is rich in catechins (epigallocatechin3Ogallate, epicatechin, (+)catechin), flavonols (rutin, quercetin), organic (citric, oxalic acid) and phenylcarboxylic acids (gallic, quinic and caffeic acid) [6,7]. The main group of compounds in green tea leaf are catechins that have antioxidant, anti-inflammatory, antimicrobial, and antifungal effects [8,9].…”

Section: Original Researchmentioning

confidence: 87%

Antioxidant, antimicrobial and antifungal activity of the obtained “Cachisept” tablets for resorption in the oral cavity for the treatment and prevention of dental caries

Maslov,

Komisarenko,

Ponomarenko

et al. 2023

CIPM

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Dental caries stands as a highly common chronic ailment affecting individuals globally, with susceptibility to this condition spanning their entire lifespan. Consequently, the search for novel medications aimed at treating and preventing dental caries holds significant importance in the field of pharmacy. The aim of the work was to study the antioxidant, antimicrobial, and antifungal activity of the obtained oral tablets “Сaсhisept” for resorption in the oral cavity for the treatment and prevention of dental caries. Materials and methods. The obtained tablets “Сaсhisept” with green tea leaf extract were the object of investigation. Antioxidant activity was established by the potentiometric method, and antimicrobial and antifungal activity was assessed by the agar well diffusion method. Results. The level of antioxidant activity for tablets “Сaсhisept” was 110.00 ± 2.20 mmol-eqv./mdry res, the obtained tablets had a high level of antioxidant activity according to Maslov’s conditional classification. Staphylococcus aureus (25.33 ± 0.50 mm), Baсillus subtilis (25.33 ± 0.50 mm) and Streptococcus mutans (25.33 ± 0.50 mm) were the most sensitive to the obtained “Сaсhisept” tablets, and the least – Proteus vulgaris (20.67 ± 0.50 mm), and it was observed strong growth inhibition in relation to the fungus Candida albicans (20.00 ± 0.50 mm). Conclusions. The presented study has found that “Сaсhisept” tablets have a high level of antioxidant activity, and also have a high antimicrobial and antifungal effect against all investigated Gram-positive, Gram-negative bacteria, and the Candida albicans fungus. Thus, “Сaсhisept” tablets can be recommended for the treatment and prevention of dental caries.

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Section: Original Researchmentioning

confidence: 87%

Antioxidant, antimicrobial and antifungal activity of the obtained “Cachisept” tablets for resorption in the oral cavity for the treatment and prevention of dental caries

Maslov,

Komisarenko,

Ponomarenko

et al. 2023

CIPM

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“…Vanillin reagent assay was then applied to determine total catechins [11]. The absorbance was measured at 505 nm.…”

Section: Qualitative Analysismentioning

confidence: 99%

Investigation the influence of biologically active compounds on the antioxidant, antibacterial and anti-inflammatory activities of red raspberry (Rubus idaeous l.) leaf extract

Maslov

Komisarenko

Ponomarenko³

et al. 2022

Current Issues in Pharmacy and Medical Sciences

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The aim of the study. To determine phenolic and organic acids compound profiles, and the antioxidant, antibacterial and anti-inflammatory activities of raspberry leaf extract. Materials and methods. The object of the study was red raspberry leaf extract. The quantity of phenolic compounds was determined by applying a spectrophotometric method of analysis, whereas organic acids content was assessed by means of the alkalimetric method, while the antioxidant activity of the obtained extract was evaluated by employing the potentiometric method, and antibacterial and antifungal activity was ascertained through the wells method, and anti-inflammatory activity was found via carrageenan – induced paw edema assay. Results. The content of phenolic compounds was 18.45±0.37 mg/ml, catechins was 10.12±0.20 mg/ml, flavonoids was 3.32±0.07 mg/ml, hydroxycinnamic acids derivatives was 2.39±0.05 mg/ml and organic acids was 7.25±0.15 mg/ml. Moreover, the antioxidant activity was 76.11±1.48 mmol-equiv./mres dry, which was higher by 32.80% than the reference drug „Ascorutin”. Staphylococcus aureus bacteria was the most sensitive to the extract (25.00±0.00 mm), whereas Pseudomonas aeruginosa was the most resistant (21.67 ±0.66 mm). Treatment with red raspberry leaf extract at 1 ml/kg showed a significant edema reduction at 1, 2 and 3 h at 38.8, 41.8 and 48.8%, compared with the saline group. Conclusian. The present study indicated that red raspberry leaf extract possesses antioxidant, antibacterial and anti-inflammatory activities. Thus, red raspberry leaves are a promising source of bioactive substances that can be used for further developing medicines in the treatment and prevention of lifestyle diseases.

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“…Green tea leaves are the major source of catechins. The content of catechins there varies from 25 to 35 % [5]. Moreover, green tea leaves contain other subgroups of phenolic compounds.…”

Section: •-mentioning

confidence: 99%

“…where С x -is the concentration of gallic acid according to the calibration curve, С × 10 -6 , g mL -1 ; V -is the volume of the extract, mL; K dil -is the dilution coefficient. The vanillin reagent assay was applied to determine the total amount of catechins [5], and the absorbance was measured at 505 nm. The calibration curve was plotted within interval concentrations of (100 -400) × 10 -6 g mL -1 , the calibration equation Y = 0.0025X -0.0851 (R 2 = 0.9951).…”

Section: Preparation Of the Standard Solutionsmentioning

confidence: 99%

Determination of the extraction frequency of green tea leaves by the antioxidant method

Maslov

Komisarenko

Kolisnyk

et al. 2022

J. Org. Pharm. Chem.

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Aim. To determine the optimal extraction frequency of green tea leaves with 60 % ethanol by the antioxidant method.Materials and methods. Chun Myn green tea leaves were the object of the study, the raw material was collected in Anhui province (China) from March to April. Dry green tea leaves were standardized according to the European Pharmacopeia 9.0. Spectrophotometry was used to quantify biologically active substances. The antioxidant activity was determined by the potentiometric method. Potentiometric measurements were performed on a HANNA 2550 pH meter (Germany) with a combined platinum EZDO 50 PO electrode (Taiwan). A UV-1000 spectrophotometer (China) was used to measure the optical density.Results and discussion. The total content of phenolic compounds was 9.60 ± 0.17, 1.30 ± 0.03 and 0.12 ± 0.002 %, catechins – 9.20 ± 0.18, 1.20 ± 0.02 and 0.07 ± 0.002 %, flavonoids – 0.27 ± 0.005, 0.04 ± 0.001, 0.005 ± 0.001, hydroxycinnamic acids – 0.49 ± 0.01, 0.07 ± 0.002 and 0.007 ± 0.001 %, dry residue – 10.75 ± 0.11, 1.59 ± 0.02 and 0.15 ± 0.002 %, the antioxidant activity was 474.08 ± 9.48, 67.70 ± 1.35 and 7.01 ± 0.14 mmol-equiv mdry res-1 for the first, second and third extraction, respectively. According to the results obtained, the optimal number of extractions of the raw material with 60 % ethanol was found to be two.Conclusions. The dynamic of extractions of biologically active substances of green tea leaves has been studied by triple extraction of the raw material to find the optimal extraction frequency; for the first time, a method for determining the extraction frequency based on the antioxidant activity of the extracts has been developed and proposed. It has been found that the optimal extraction rate is 2 times. The results obtained will be used in the further production of herbal medicines, dietary supplements, and cosmetic products with a green tea extract.

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Determination of catechins in green tea leaves by HPLC compared to spectrophotometry

Cited by 8 publications

References 16 publications

Antioxidant, antimicrobial and antifungal activity of the obtained “Cachisept” tablets for resorption in the oral cavity for the treatment and prevention of dental caries

Antioxidant, antimicrobial and antifungal activity of the obtained “Cachisept” tablets for resorption in the oral cavity for the treatment and prevention of dental caries

Investigation the influence of biologically active compounds on the antioxidant, antibacterial and anti-inflammatory activities of red raspberry (Rubus idaeous l.) leaf extract

Determination of the extraction frequency of green tea leaves by the antioxidant method

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