The study of the effect of ethyl alcohol concentrations on the antioxidant activity of ascorbic acid solutions

Maslov, O. Yu.; Kolisnyk, S. V.; Ponomarenko, Svitlana V.; Ahmedov, Elshan Yunis Ogli; Shovkova, Z. V.

doi:10.24959/ophcj.21.231947

Cited by 7 publications

(6 citation statements)

References 4 publications

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“…In our opinion, the results of their study on the determination of the AOA of the extract may not be reliable as they did not take into account the AOA of ethyl alcohol. In our previous studies [18], it was found that ethyl alcohol contributes to overall level of antioxidant activity.…”

Section: Introductionmentioning

confidence: 87%

“…The shift of potential is explained by the change of ratio of o of the mediator system. The following equation was used to calcu mmol-eqv./mres dry weight [18,23]:…”

Section: Methodsmentioning

confidence: 99%

“…The shift of potential is explained by the change of ratio of oxidized and reduced forms of the mediator system. The following equation was used to calculate AOA and expressed as mmol-eqv./m res dry weight [18,23]: raw material in order to understand antioxidant capacity of it and f information in developing drug, dietary supplements and cosmeto…”

Section: The Procedures Of Determining the Level Of Aoamentioning

confidence: 99%

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Study of total antioxidant activity of green tea leaves (Camellia sinensis L.)

et al. 2022

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Summary Introduction: There is a high interest in creating medicines, dietary supplements, cosmetics including plant extract with antioxidant activity. For understanding whether plant extract has a maximum level of antioxidant activity it is important to know the total antioxidant activity of raw material. Objective: The main goal of study was to find out the green tea leaves total antioxidant activity. Methods: The antioxidant activity was measured by potentiometric method. Total phenolic, flavonoids, catechins and hydrocinnamic acids derivatives were quantified using Folin-Ciocalteu, aluminium chloride, vanillin and sodium molibdate methods, respectively. Results: The green tea leaves total antioxidant activity was 660.75 mmol-eqv./mres. dry weight. A significant correlation was observed between the amount of phytochemicals and antioxidant activity, which indicated its main role in antioxidant activity. Conclusion: The research showed that the green rea leaves possess a high value of antioxidant activity and it is a good source of phenolic constituents.

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Section: Introductionmentioning

confidence: 87%

“…The shift of potential is explained by the change of ratio of o of the mediator system. The following equation was used to calcu mmol-eqv./mres dry weight [18,23]:…”

Section: Methodsmentioning

confidence: 99%

Section: The Procedures Of Determining the Level Of Aoamentioning

confidence: 99%

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Study of total antioxidant activity of green tea leaves (Camellia sinensis L.)

et al. 2022

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“…Antioxidant activity. The antioxidant activity was assessed using the potentiometric method [14,15,16]. Antioxidant activity was calculated according to the following equation and expressed as mmol-equiv./L .…”

Section: Methodsmentioning

confidence: 99%

Investigation of antioxidant interaction between ascorbic acid and rutin in medicine “Ascorutin”

Maslov,

Komisarenko,

Kolisnyk

et al. 2024

Current issues in pharmacy and medicine: science and practice

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Phytomedicines are combined pharmaceuticals containing various groups of phenolic compounds. It is known that phenolic compounds are powerful antioxidants, but their level of antioxidant activity during interactions has not been previously studied. The aim of the work was to investigate interaction between ascorbic acid and rutin in medicine “Ascorutin”. Materials and methods. The objects of the study were one series of tablets “Ascorutin,” rutin, and ascorbic acid. The level of antioxidant activity was measured by the potentiometric method. Results. The level of antioxidant activity of model solutions of rutin was 265.51 ± 1.00 mmol-eq./L, ascorbic acid – 99.00 ± 1.00 mmol-eq./L, a combination of rutin and ascorbic acid – 255.60 ± 1.00 mmol-eq./L, and medicine “Ascorutin” – 256.52 ± 1.00 mmol-eq./L. An experimental value of the level of antioxidant activity of “Ascorutin”, the combination of rutin and ascorbic acid was 30 % less than theoretical values. Conclusions. The antioxidant interaction of ascorbic acid and rutin in “Ascorutin” medicine has been studied. It has been established that the combination of rutin and ascorbic acid has an antagonistic effect. The findings of this research will serve as a stimulus for exploring the interaction of bioactive substances and selecting their optimal combinations for the development of pharmaceuticals, special food products, food, and cosmetic products.

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“…where A -is the absorbance of the solution analyzed; 188 -is the specific absorption of chlorogenic acid; V -is the volume of the extract, mL; K dil -is the dilution coefficient. The antioxidant activity of the extracts was measured by the potentiometric method [12] calculated according to the following equation, and expressed as mmol-equiv m dry res.…”

Section: Preparation Of the Standard Solutionsmentioning

confidence: 99%

Determination of the extraction frequency of green tea leaves by the antioxidant method

Maslov

Komisarenko

Kolisnyk

et al. 2022

J. Org. Pharm. Chem.

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Aim. To determine the optimal extraction frequency of green tea leaves with 60 % ethanol by the antioxidant method.Materials and methods. Chun Myn green tea leaves were the object of the study, the raw material was collected in Anhui province (China) from March to April. Dry green tea leaves were standardized according to the European Pharmacopeia 9.0. Spectrophotometry was used to quantify biologically active substances. The antioxidant activity was determined by the potentiometric method. Potentiometric measurements were performed on a HANNA 2550 pH meter (Germany) with a combined platinum EZDO 50 PO electrode (Taiwan). A UV-1000 spectrophotometer (China) was used to measure the optical density.Results and discussion. The total content of phenolic compounds was 9.60 ± 0.17, 1.30 ± 0.03 and 0.12 ± 0.002 %, catechins – 9.20 ± 0.18, 1.20 ± 0.02 and 0.07 ± 0.002 %, flavonoids – 0.27 ± 0.005, 0.04 ± 0.001, 0.005 ± 0.001, hydroxycinnamic acids – 0.49 ± 0.01, 0.07 ± 0.002 and 0.007 ± 0.001 %, dry residue – 10.75 ± 0.11, 1.59 ± 0.02 and 0.15 ± 0.002 %, the antioxidant activity was 474.08 ± 9.48, 67.70 ± 1.35 and 7.01 ± 0.14 mmol-equiv mdry res-1 for the first, second and third extraction, respectively. According to the results obtained, the optimal number of extractions of the raw material with 60 % ethanol was found to be two.Conclusions. The dynamic of extractions of biologically active substances of green tea leaves has been studied by triple extraction of the raw material to find the optimal extraction frequency; for the first time, a method for determining the extraction frequency based on the antioxidant activity of the extracts has been developed and proposed. It has been found that the optimal extraction rate is 2 times. The results obtained will be used in the further production of herbal medicines, dietary supplements, and cosmetic products with a green tea extract.

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The study of the effect of ethyl alcohol concentrations on the antioxidant activity of ascorbic acid solutions

Cited by 7 publications

References 4 publications

Study of total antioxidant activity of green tea leaves (Camellia sinensis L.)

Study of total antioxidant activity of green tea leaves (Camellia sinensis L.)

Investigation of antioxidant interaction between ascorbic acid and rutin in medicine “Ascorutin”

Determination of the extraction frequency of green tea leaves by the antioxidant method

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