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A method was developed for detection of group A arboviruses, Sindbis and Venezuelan equine encephalomyelitis (VEE), in the salivary glands of experimentally infected Aedes aegypti mosquitoes by direct immunofluorescence with counter staining. During the period shortly after infection, the virus was localized in distal parts of the lateral lobules of the salivary glands, whereas during the time of maximum multiplication it was concentrated in the anterior parts. Detection of the virus in the salivary glands by immunofluorescent staining techniques reflected the ability of the mosquito to transmit infection by bite.

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SummaryIn 1969--1971 nine virus strains were isolated from Ixodes putus ticks and were shown to be antigenically related to Kemerovo virus by complement-fixation tests. By neutralization tests, no antigenic relations were revealed. Five virus strains were isolated on Tyuleniy island, Sakhalinsk region (1969--1971); three virus strains on Ariy Kamen' rock in the vicinity of the Pacific coast of Bering island, Commodore islands, Kamchatsk region (1970--1971) and one on the SouthEastern coast of Chukotka, Magadansk region (1971). The isolated virus was named after the place of its first isolation, the sea of Okhotsk. A serological survey of 1066 birds in the Far East indicated their active role in circulation of the virus, since complement-fixing antibodies were found in 3.9 per cent of Uria aalge (common murres), 14.6 per cent of Fulmarus glacialis (fulmars) and 0.7 per cent of Phalacrocorax pelagicus (pelagic cormorants).Complement-fixing and virus-neutralizing antibodies were found in birds from Commodore islands in Kamchatsk region and from Iona, Tyuleniy, Moneron islands in Sakhalinsk region. IntroduetionTicks were collected in 1969--1971 on: i) Tyuleniy island, Zaliv Terpeniya Patience bay) of the sea of Okhotsk, S~khalinsk region (12) ; ii) Ariy Kamen' rock situated close to the Pacific coast of Bering island, Commodore islands, Kamchatsk region (16); iii) the South-Eastern coast of Chukotka, Magadansk region.

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