This research aims to develop a method for selection based on myostatin gene markers. The experiment was conducted in Bali cattle. Blood samples were collected from 100 cattle irrespective of age. PCR-RFLP was applied with primers for amplifying the 1346bp of the Myostatin gene were (F) 5'-CCCTACAGAGGCCACTTCAA-3' and (R) 5'-CTCGCTGTTCTCATTCAGATC-3', sand HaeIII restriction enzymes. PCR-RFLP results showed that not all genotypes present in the population, despite populations that were observed quite polymorphic (PIC = 49.5%) with the conditions of gene frequencies. The results showed that almost all variables influenced by myostatin genotype where the genotype BB is better, except the chest girth showed a better result in genotype AB. It suggested that MSTN is strong candidate gene that influenced growth traits. Moreover, it is also expected that this MSTN could be used in marker-assisted selection.

Latar Belakang:Komplikasi kehamilan adalah kegawat daruratan obstetrik yang dapat menyebabkan kematian pada ibu dan bayi. Penyebab komplikasi kehamilan diantaranya kurangnya pengetahuan ibu tentang deteksi dini kehamilannya, usia pasien < 20 tahun dan > 35 tahun serta anak lebih dari 3.Tujuan :Penelitian ini bertujuan untuk mengetahui hubungan pengetahuan, usia dan paritas dengan kejadian komplikasi kehamilan pada ibu hamil trimester III.Metode Penelitian:Jenis penelitian observasional analitik dengan pendekatan cross sectional. Teknik pengambilan sampel menggunakan purposive sampling, sehingga sampel adalah ibu hamil trimester III yang berkunjung di Rumah Sakit Ibu dan Anak Aisyiyah Samarinda berjumlah 84 orang. Analisis yang digunakan uji chi square.Hasil : Hasil penelitian menunjukkan terdapat responden yang memiliki pengetahuan kurang baik, terdapat usia berisiko antara < 20 tahun dan > 35 tahun, terdapat paritas berisiko > 3 orang anak dan komplikasi kehamilan berupa hipertensi, anemia, preeklempsia dan plasenta previa. Ada hubungan pengetahuan dengan kejadian komplikasi kehamilan (p value : 0,001 < α : 0,05 dan odds ratio : 6,800 > 1). Ada hubungan usia dengan kejadian komplikasi kehamilan (p value : 0,003 < α : 0,05 dan odds ratio : 5,837 > 1). Ada hubungan paritas dengan kejadian komplikasi kehamilan (p value : 0,002 < α : 0,05 dan odds ratio : 6,250 > 1).Kesimpulan: Terdapat pengetahuan kurang baik berjumlah 27 responden (32,1%), usia berisiko (< 20 tahun dan ≥ 35 tahun) berjumlah 25 responden (29,8%), paritas berisiko (1 atau ≥ 3 orang anak) berjumlah 21 responden (25%) dan ada komplikasi kehamilan berjumlah 18 responden (21,4%), Ada hubungan pengetahuan, usia dan paritas dengan kejadian komplikasi kehamilan pada ibu hamil trimester III di Rumah Sakit Ibu dan Anak Aisyiyah Samarinda.

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Anxiety is a very familiar term that describes a state of worry, anxiety, fear, and in peace with various physical grievances. Medical students experience a level of mental exhaustion that includes anxiety. Medical faculty students have a hectic schedule of lectures, tutorial activities, practicum, lab skills and the demands to study independently outside those hours so that the pressure on physical and mental conditions is relatively more severe than other education. In addition to learning activities, medical students also conduct Objective Structural Clinical Examination (OSCE) exams as an instrument testing clinical skill of medical students. The many thoughts about the material to be learned between theory, clinical skills in the OSCE exam, osce atmosphere, OSCE testers observing participants directly, unpreparedness following the OSCE, OSCE mechanism and the same time interval of each stase make osce known as a test that causes quite high anxiety. This research aims to find out the picture of anxiety levels of students of The Faculty of Medicine Universitas Mulawarman before facing the OSCE. This research is a descriptive observational study with cross-sectional design. Sampling using stratified random sampling techniques. This level of anxiety was measured using the English version of the Hamilton Anxiety Rating Scale (HARS) questionnaire which was translated into Indonesian with 14 items. The sample was obtained by 96 students. In this study, 27 students (28.12%) with mild anxiety levels, 29 students (30.21%) with mild to moderate anxiety levels, 40 students (41.67%) with moderate to severe anxiety levels.

BACKGROUND: The depletion of glucose transporter 4 (GLUT-4) affects blood glucose level in the type 2 diabetes mellitus (T2DM) patient. Herbals supplementation such as Artemisia annua is known to posses an antidiabetic potency, but its effect on long term glucose control marker, glicated hemoglobin (HbA1C) and muscle GLUT-4 expression still has not been investigated.METHODS: Twenty Wistar rats were divided into four groups (five rats per group). The C1 group was consist of healthy control rats and C2 group was consist of diabetic control rats. Diabetic condition was induced by giving high lard fat diet for 28 days, followed with 30 mg/kgBW of Streptozotocin injection at the 29th day. Meanwhile, T1 and T2 group were consist of diabetic rats, which were supplemented with 50 mg/kgBW and 100 mg/kgBW of Artemisia annua leaf extract, respectively. GLUT-4 expression and HbA1C level were measured at the 14th day post-treatment.RESULTS: The GLUT-4 was expressed 1.8 and two times higher in T1 and T2 group, respectively, compared to C2 group. Meanwhile, HbA1C level in C2 group was two times higher than C1 group (11.95±2.52 compared to 5.61±2.69, p<0.01). Further, the administration of 100 mg/ kgBW Artemisia annua extract caused a slight reduction of HbA1C (11.95±2.52 compared to 8.18±3.53, p>0.05)CONCLUSION: Muscle cell’s GLUT-4 in T1 and T2 group was expressed increasingly and significantly different compared to the C2 group. HbA1C level in T2 was slightly reduced although no significant different compared to C2 group.KEYWORDS: Artemisia annua, GLUT-4, HbA1C, type 2 diabetes mellitus

Background: The prevalence of hypertension in type 2 diabetic patients is 1,5-3 times higher than it is in nondiabetic individuals This chronic condition accelerates macrovascular complications. Research about risk factors of hypertension in type 2 diabetic patients is still rare because its causes multifactorial. The objectives of this research is to explain the risk factors affecting hypertension in type 2 diabetic patients.Methods: An observational studies with case-control study design in Primary Healthcare Centers patients in Pati District. Patients with hypertension in type 2 diabetes are the case, while the type 2 diabetes patients without hypertension are the control. There were 57 cases and 57 controls included. Data were obtained from medical records and qualitative interviews. Chi-square test in bivariate and multiple logistic regression in multivariate analysis.Results: Factors that influence hypertension in type 2 diabetic patients were physical activity (OR=6,4; 95% CI: 2,18 - 18,77; p=0,001), diabetes duration ≥ 5 years (OR=5,4; 95% CI: 1,97 - 14,704; p=0,001), and medication adherence (OR=3,6; 95% CI: 1,32 - 9,83; p=0,012). Other risk factors i.e age ≥45 years, male, diet compliance, history of hypertension, smoking, salt consumption, coffee consumption, and sleep duration were not significantly influenced.Conclusion: In this study, physical activity, diabetes duration, and DM medication adherence are risk factors that influence of hypertension in type 2 diabetic patients.

Kabupaten Bandung Barat adalah salah satu kabupaten di Provinsi Jawa Barat dengan potensi gerakan tanah yang tinggi, sehingga sangat rentan terhadap kejadian tanah longsor. Hal ini dipicu oleh kondisi topografi yang beragam dan memungkinkan kejadian ini akan terus terjadi di masa depan. Salah satu metode yang sering digunakan dalam pemetaan kerentanan tanah longsor adalah frequency ratio. Metode ini bertujuan untuk mengidentifikasi wilayah rentan tanah longsor berdasarkan data kejadian longsor di masa lalu yang pada gilirannya dapat dijadikan parameter untuk mitigasi bencana longsor. Metode ini diaplikasikan menggunakan aplikasi GIS dengan data-data sekunder seperti DEM, peta tutupan lahan, data curah hujan, peta geologi, dan peta klasifikasi tanah. Data tersebut merepresentasikan faktor-faktor yang memengaruhi tanah longsor yaitu kemiringan lereng, elevasi, arah kemiringan lereng, tutupan lahan, curah hujan, jenis tanah, jarak sesar, dan batuan geologi. Faktorfaktor tersebut ditumpangtindihkan menjadi peta raster (20 m) dan menghasilkan nilai frequency ratio. Nilai tersebut diklasifikasikan menjadi 5 zona kerentanan longsor yaitu tidak rentan, sedikit rentan, cukup rentan, rentan, dan sangat rentan. Hasil penelitian ini menunjukkan terdapat 7 faktor pengkondisi tanah longsor yang memengaruhi kerentanan tanah longsor. Hasil uji akurasi ini menghasilkan peta kerentanan tanah longsor dengan nilai akurasi sebesar 79,7% sehingga dapat diterapkan dalam upaya mitigasi tanah longsor.