Artificial Insemination (AI) using sexed semen produce sex of calf as expected. There are various methods of sexed semen has been found. The aim of this research is to determine the effect of sexed semen methods using egg white sedimentation and percoll density gradient centrifugation toward motility and ratio of X and Y sperm Filial Ongole Cattle. Semen was collected using Artificial Vagina (AV) from Filial Ongole Cattle, evaluated and then separated using egg white sedimentation and percoll density gradient centrifugation technique. Only fresh semen with a minimum of 70% individual motile sperm and 2+ mass motility used in this study. Andromed as a based extender was diluted using aquabidest with 1:4 ratio. The obtained data were analyzed with analysis of variance (ANOVA) and continued by Duncan test if there was significant or very significant different. The result showed that the sexing methods (non sexing sperm, upper and under fraction using percoll density gradient centrifugation, upper and under fraction using egg white sedimentation technique) had very significant effect (P<0.01) on motility (64.25±3.94%; 48.55±8.28%; 53±7.93%; 56.9±8.22%; 49.75±8.19% respectively) and significant effect (P<0.05) on the ratio of X and Y sperm. The ratio of X and Y using percoll density gradient centrifugation and egg white sedimentation methods for the upper fraction were 28.0±2.26%:72.0±2.26% and 77.5±1.26%:22.5±1.26%. The ratio of X and Y using percoll density gradient centrifugation and egg white sedimentation technique for the under fraction were 69.0±15.35%: 26.0±4.37% and 22.9±1.44%: 77.1±1.44%. The study concludes that the sexed with egg white sedimentation technique is better than percoll density gradient centrifugation on sperm motility and ratio of X and Y sperm.

Penelitian ini bertujuan untuk mengetahui kualitas semen segar kambing Kacang pada penyimpanan yang berbeda pada suhu ruang dengan menggunakan pengencer tris aminomethan kuning telur. Penelitian ini dilakukan di Laboratorium Fakultas Peternakan Universitas Kanjuruhan Malang. Metode penelitian yang digunakan adalah metode percobaan dengan menggunakan Rancangan Acak Lengkap (RAL). Setiap perlakuan semen diulang sebanyak 5 kali. Variabel yang diamati antara lain motilitas, viabilitas dan abnormalitas. Data dianalisis menggunakan Analisis Varian. Apabila diantara perlakuan terdapat perbedaan maka dilanjutkan dengan Uji Beda Nyata Terkecil. Berdasarkan hasil penelitian menunjukkan bahwa kualitas spermatozoa kambing Kacang pada suhu ruang dengan lama simpan yang berbeda dengan menggunakan pengencer menunjukkan adanya perbedaan yang sangat nyata (P<0,01). Pada pengamatan motilitas dan viabilitas spermatozoa kambing Kacang pada suhu ruang  menggunakan pengencer menunjukkan adanya perbedaan yang sangat nyata (P<0,01). Tetapi pada pengamatan abnormalitas spermatozoa kambing Kacang pada suhu ruang tidak menunjukkan adanya pengaruh penggunaan pengencer serta tidak terdapat pengaruh antara lama simpan dan penggunaan pengencer terhadap abnormalitas spermatozoa kambing Kacang (P>0,01). Namun terdapat pengaruh yang sangat nyata lama simpan terhadap abnormalitas spermatozoa kambing Kacang. Kesimpulan yang diperoleh dari hasil penelitian yaitu lama simpan yang berbeda pada suhu ruang menggunakan tris aminomethan kuning telur terdapat pengaruh sangat nyata terhadap kualitas semen segar kambing Kacang. Berdasarkan hasil penelitian ini maka disarankan agar penggunaan kuning telur sebagai pengencer tidak lebih dari 9 jam karena dapat menurunkan motilitas dan viabilitas spermatozoa.Kata kunci : Lama Simpan, Kambing Kacang, Pengencer, Spermatozoa, Kualitas Semen

This study aims to investigate the quality of Simental frozen semen based on the temperatures and duration of thawing. The materials used in this study were frozen semen of Simental cattle. The study used a laboratory experiment using a completely randomized design (CRD) factorial with 6 treatments and 10 replications. The thawing temperatures were 37°C and 25°C and the durations of thawing were 7 seconds, 15 seconds and 30 seconds. The variables observed in this study were the motility, viability and abnormality of Simental frozen semen. The data were analyzed using analysis of variance (ANOVA). The difference in advanced test was done by using the smallest Real Difference Test. The results showed that the temperature and duration of thawing and the interaction of temperature and duration of thawing provide a highly significant difference (P <0.01) on motility, viability and abnormality of Simental frozen semen. The highest motility (45,5%) and viability (75,65%) were found on 37°C and during 30 seconds of thawing, and the lowest abnormality was 4,71%. This study concludes that the optimal thawing temperature and the lenght of thawing for Simental frozen semen is 37°C and 30 seconds respectively.Keywords:thawing, sperm, motility, viability, temperature, abnormality PENDAHULUANSapi Simental memiliki persentasi karkas tinggi dan dapat difungsikan sebagai sapi potong dan perah dengan pertambahan bobot badan berkisar 0,6 sampai 1,5 kg per hari (Yunus, 2014). Sapi ini memiliki ciri-ciri warna kuning sampai merah serta memiliki warna putih pada muka, rambut ekor dan dada serta tidak bertanduk.Pemeriksaan atau evaluasi semen segar terbagi menjadi 2 kelompok, yaitu pemeriksaan secara makroskopik dan pemeriksaan mikroskopik. Pemeriksaan secara makroskopi meliputi warna semen, bau semen, volume semen, konsistensi dan pH semen. Sedangkan pemeriksaan mikroskopik meliputi motilitas massa, motilitas individu, viabilitas spermatozoa, konsentrasi dan abnormalitas spermatozoa (Kartasudjana,2001;Yendraliza, 2008).Pengenceran dan penyimpanan bertujuan untuk menurunkan aktifitas metabolisme yang berlebihan agar dapat memperpanjang waktu hidup spermatozoa didalamnya, bahan pengencer yang digunakan hendaknya mampu menyediakan zat-zat makanan sebagai sumber energi, mencegah stress dingin, mencegah pertumbuhan mikroorganisme dan sebagai buffer (Hafez, 2008).

Artificial insemination using semen Sexing aim to improve the genetic quality and to get calf with gender according to expectations. Sexing using density gradient centrifugation percoll and sedimentation egg whites have been successfully frozen. This study aims to determine the effect of sexing process using density gradient centrifugation percoll and Egg white sedimentation on motility and membrane damage spermatozoa. The material used is bull Filial Ongole semen have motility which has more than 65%. Treatment ; T0 = without sexing; T1 = Sexing. Each treatment was conducted repeat 10 times. The design used the Block Design and descriptive analytics. Media diluent used is andromed @ . Percentage motility by using a Light microscope magnification 100X. while damage to the membrane using Electrone Scanning microscope (SEM) with a magnification of 10,000 X. The result is the percentage motility ; T0 = 64,25 ± 3,94%; T1 After sexing , X-sperm and Y-sperm = 53± 7,93 % dan 48,55 ± 8,28%, . Motility before freezing at T0 = 59,75 ± 4,48%. T1 before Freezing , X-sperm and Y-sperm = 47 ± 6,13% dan 42,1 ± 7,36% and Post thawing motility X-sperm and Y-sperm T0 = 44,2± 2,79%, T1 = 31,45± 7,20% and 27,45± 8,69%. On Raw semen looked membrane intact spermatozoa. Y sperm after sexing were damaged membranes of the head and in spite of the sperm head to tail, whereas the X sperm membrane damage not appear. After freezing both the control and the results of many spermatozoa sexing damaged membranes and head with tail no intact.

The purpose of this research is to evaluate the implementation of MBKM in the learning process using the case method and team based project implemented by the study program at Unikama. This research is a descriptive research with qualitative and quantitative approaches. The research method used is a survey method using a questionnaire. The research subjects were determined based on the research objectives, namely the entire academic community and stakeholders in the Unikama environment. The results of this study indicate that the implementation of the case method and Team Based Project is one method that is in accordance with the MBKM program and is actually able to provide opportunities to improve student skills, namely Creativity Skills, Critical Thinking Skills, Communication Skills, and Collaboration Skills (4C), so that better quality of graduates. The implementation of MBKM using Case Method and Team Base Project Learning has been carried out by lecturers

Abstrak   Tujuan dari penelitian ini adalah untuk mengetahui pengaruh penambahan level ekstrak bawang merah terhadap kualitas semen cair kambing peranakan ettawa (PE) dengan menggunakan pengencer ringer’s dextrose. Materi yang digunakan adalah semen segar kamping peranakan ettawa (PE), Ringer's Dextrose, dan ekstrak bawang merah. Metode penelitian yang digunakan adalah eksperimental dengan menggunakan analisis statistik RAL (Rancangan Acak Lengkap). Pada penentuan tingkat ekstrak bawang dalam Dextrosperlich Ringer penguat adalah suhu ruang penyimpanan, hasil terbaik yang diketahui pada motilitas individu (P <0,01) adalah tambahan 2% sebanyak 71,87 ± 2,5. Dalam menentukan tingkat Ekstraksi ekstrak bawang Ringer Dextrose room temperature, hasil terbaik yang diketahui dalam survival (P <0,01) adalah penambahan 2% sebanyak 90,63 ± 0,97. Pengaruh kadar ekstrak bawang dalam pengencerRinger's Dextrose juga memberikan kelainan (P <0,05) di samping 2% sebanyak 0,61 ± 0,18. Kesimpulan dari penelitian ini adalah penambahan ekstrak bawang bombay pada diluent Ringer's Dextrose memberikan pengaruh nyata terhadap motilitas, viabilitas. Perlu penelitian lebih lanjut mengenai lama penyimpanan untuk mengetahui bagaimana ekstrak bawang bisa dapat mempertahankan kehidupan spermatozoa.   Abstract   The purpose of this research was to know and study the influence of level of different onion extract in diluent Ringer’s Dextrose to the quality of goat Ettawa cross breed’s semen room temperature storage. The material which was used is fresh semen Ettawa cross breed’s, Ringer’s Dextrose, Onion extract. The research method which is used is experimental by using statistical analysis of RAL (Complete Random Design). On the determination of the level of onion extract in a diluent Ringer’s Dextrose which is store room temperature, best results were known on motility of individuals (P<0,01) is in addition of 2% as much as 71.87±2.5.In determining the level of onion extract dilution Ringer Dextrose room temperature, the best results are known in survival ( P < 0.01 ) was the addition of 2 % as much as 90.63 ± 0.97. The influence of level of onion extract in diluentsRinger’s Dextrose is also giving abnormality (P<0,05) in addition of 2% as much as 0.61±0.18.The conclusion of this research is the addition of onion extract in diluent Ringer’s Dextrose give the real influence to motility, viability. It needs further research concerning the length of storage in order to find out how onion extract could sustain the life of spermatozoa.

Successfull of artificial insemination by using frozen semen is relatively low, because of decreasing of sperm motility after frozen process. In this study, the chilled semen was used to increase the succesful of artificial insemination. The purpose of this study was to evaluate the successful of artficial insemination by using chilled semen on Brahman Cross cows. In this study the semen was diluted with two kinds of extender were CEP without BSA + egg white albumin and tris aminomethane without raffinose. The material used on this study were 45 brahman cross x cows wich were inseminated by chilled semen using CEP without albumin +egg white (T1) and 45 brahman cross X cows were inseminated with chilled semen using Tris Aminomethane Without raffinose (T2). The chilled semen have the individual motility at least 40% to be inseminated to the female recipient which were stored at 4°C. The parameter observed were non return rate (NRR) and conception rate (CR). The data was analyzed by using chi-square analysis and t-test analysis. T-test result showed that there were significant differences (P<0.01) between T2 and T1 on conception rate and NRR. Then chi square result showed that there were significant differences T1 (P<0.05) and T2 (P<0.01) on conception rate wichs is the CR for T1 Was 66,7%, while on T2 was 62,2 % where the expected pregnancy rate was 60%. it indicates that chilled semen both using tris aminomethane extender and CEP extender can be used to be inseminated to the cows . In conclusions, the results clearly showed that chilled semen can be successfully inseminated to the Brahman cross cows.

The Ujian Akhir Sekolah Berstandar Nasional (UASBN) educational game is designed as a means of training for students to face school final exams in 6th grade. Before implementing this educational game, it must be tested first related to user acceptance of the technology created or from the human-computer interaction (HCI) side. A total of 173 students tested this educational game, and then data was collected based on the criteria from the Technology Acceptance Model (TAM). The measurement criteria include Perceived Usefulness (TPU) and Perceived Ease of Use (TPE) variables, each of which has aspects of functionality (TFL), accessibility (TAC), and Learning Goal Orientation (TLGO). In contrast, the dependent variable is Behaviour Intention (TBI). The SEM (AMOS) and SPSS statistical packages were used to analyze this HCI evaluation study. A questionnaire with 21 questions was used to collect the necessary data for analysis. The findings of the estimation demonstrate that each component of TAM contributes pretty significantly. In total, user acceptance of the UASBN educational game is a TPE variable contributing 39% to the UASBN human-computer interaction game. Meanwhile, the TPU variable and its variants contributed 73% to the human-computer interaction game UASBN, and Meanwhile, TBI gave a role of 50.4% to the human-computer interaction game UASBN education. This shows that human-computer interaction with educational games through the technology acceptance model criteria contributed 50.4%, while 49.6% was contributed by other variables not raised in this research. This demonstrates that the educational requirements for student gaming training can be satisfactorily met by users and produce favorable outcomes.