The Motility and Ratio of X and Y Sperm Filial Ongole Cattle Using Different Sexed Semen Methods

Kusumawati, Enike Dwi; Isnaini, Nurul; Yekti, Aulia Puspita Anugra; Luthfi, Muchamad; Affandhy, Lukman; Pamungkas, Dicky; Kuswati, .; Ridhowi, Aswah; Sudarwati, Herni; Rahadi, Syam; Rahayu, Sri; Susilawati, Trinil

doi:10.3844/ajavsp.2019.111.114

Cited by 11 publications

(20 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Finally, remove the supernatant, and leave 1 ml of precipitate in the tube. Then diluted to the total concentration of 25 million/straw and cooled at 5 o C [7,8].…”

Section: Percoll Density Gradient Centrifugation Sexingmentioning

confidence: 99%

See 1 more Smart Citation

Relationship of motility and acrosome reaction on sexing sperm in Ongole Crossbred bull

2022

View full text Add to dashboard Cite

Artificial insemination using sexing semen is expected to produce calves with the desired sex. One sexing sperm method is the percoll density gradient centrifugation method. This study aimed to determine the changes and the relationship between motility and acrosome reaction after sexing process using percoll density gradient centrifugation. The material used was semen of ±5 years old Ongole crossbred bull with a bodyweight of ±700 kg as many as three bulls with mass motility 2+ and individual motility 70%. The method used was to compare fresh semen with sexed semen after the cooling process. Parameters measured were motility characters using CASA analysis, which included motility parameters, progressive motility, capacitation, and no acrosome reaction. Statistical analysis used paired T-test to distinguish among fresh semen, after sexing and cooling process. In comparison, regression and correlation were used to analyze the relationship of capacitation and hyperactivation sperm with no acrosomal reaction with motility and progressive motility. The results showed that motility and progressive motility decreased after the sexing and cooling process. Meanwhile, the acrosomal reaction, capacitation, and hyperactivity increased.

show abstract

“…Finally, remove the supernatant, and leave 1 ml of precipitate in the tube. Then diluted to the total concentration of 25 million/straw and cooled at 5 o C [7,8].…”

Section: Percoll Density Gradient Centrifugation Sexingmentioning

confidence: 99%

“…The sexed semen has then calculated the concentration using a hemocytometer and diluted to a concentration of 25 million/straw [9]. Then followed with the process of cooling to a temperature of 5C in the refrigerator [7].…”

Section: Dilution and Cooling Of Semenmentioning

confidence: 99%

Relationship of motility and acrosome reaction on sexing sperm in Ongole Crossbred bull

2022

View full text Add to dashboard Cite

show abstract

“…Hal ini disebabkan karena spermatozoa mengalami penurunan setelah proses sexing dan pendinginan, karena pada proses sexing dilakukan sentrifugasi, sehingga terjadi proses pergesekan antara spermatozoa dengan medium pengencer, sesama spermatozoa dan juga tabung reaksi. Berdasarkan pendapat Kusumawati et al (2019) motilitas spermatozoa sexing mengalami penurunan motilitas hingga 48+8,28% pada lapisan atas dan 53+7,93% pada lapisan bawah. Susilawati et al (2014) menyatakan bahwa spermatozoa setelah dilakukan sexing menggunakan metode Sentrifugasi Gradien Densitas Percoll akan mengalami penurunan kualitas disebabkan karena terjadinya kerusakan membran spermatozoa.…”

Section: Persentase Motilitas Dan Motilitas Progresif Setelah Sexing Dan Pendinginanunclassified

Karakter Motilitas Spermatozoa Hasil Sexing pada Sapi Peranakan Ongole dengan Volume Awal yang Berbeda

et al. 2020

Self Cite

View full text Add to dashboard Cite

ABSTRAK. Sexing spermatozoa bertujuan untuk mengatur jenis kelamin sesuai harapan. Salah satu metode sexing adalah dengan menggunakan Sentrifugasi Gradien Densitas Percoll. Tujuan dari penelitian ini adalah untuk mengetahui berbagai parameter motilitas menggunakan Computer-assisted Sperm Analysis dan proporsi spermatozoa X dan Y menggunakan metode Sentrifugasi Gradien Densitas Percoll dengan volume awal yang berbeda. Penelitian dilakukan di Loka Penelitian Sapi Potong Grati, Pasuruan, pada bulan Januari sampai Maret 2020. Materi yang digunakan adalah Semen Sapi Peranakan Ongole berumur ±5 tahun dan bobot badan ±700 kg sebanyak 3 ekor, motilitas masa ≥ 2+ dan motilitas individu ≥ 70%. Metode yang digunakan adalah eksperimental menggunakan Rancangan Acak Kelompok (RAK) dengan 3 perlakuan volume saat sexing yaitu P1= 1 ml, P2= 1,5 ml dan P3= 2 ml dengan ulangan 11 kali. Ulangan juga berfungsi sebagai kelompok (block). Hasil penelitian menunjukkan bahwa setelah dilakukan sexing dan pendinginan hingga 5oC persentase motilitas adalah P1:71,02+10,08 %; P2: 79,63+8,65 % dan P3: 83,38+6,67 %, sedangkan motilitas progresif pada P1: 47,68+8,71%; P2: 59,61+7,26 % dan P3: 62,21+6,74%. Curvilinear Velocity (VCL) pada P1: 50,9±7,73 µm/s, P2: 55,2±5,03 µm/s dan P3: 53,2±5,97%. Straight-line Velocity (VSL) pada P1: 20,8±8,19 µm/s; P2: 21,6±6,02 µm/s; P3: 22,1±5,77 µm/s, sedangkan Average Path Velocity (VAP) pada P1: 30,2±7,82 µm/s; P2: 32,5±6,14 µm/s dan P3 : 31,5±6,18 µm/s. Linearity (LIN) pada P1: 40,2±12,26%;P2: 39,1±10,31%;P3: 39,8±7,25%. Straightness (STR) pada P1: 67,2±11,20%; P2: 65,7±10,06%;P3: 67,2±7,92% sedangkan Wobble (WOB) pada P1: 59±9,49%; P2: 58,8±8,63% dan P3: 59,1±7,74%. Kesimpulan dari penelitian adalah motilitas dan motilitas progresif spermatozoa pada sampel dengan volume awal 2 ml lebih baik dibandingkan dengan sampel dengan volume awal 1 ml dan 1,5 ml. (Characteristics of Sexing Spermatozoa Motility in Ongole Cattle with Different Initial Volume) ABSTRACT. Sperm sexing is a technique of sorting a specific type of sperm cell to fertilize the egg cell. One of the sexing methods that can be used for spermatozoa sexing is percoll gradient density centrifugation. This research aims to find out various motility parameters using Computer-assisted Sperm Analysis and the proportion of spermatozoa X and Y using density gradient centrifugation method percoll with different initial volume. The study was conducted at Grati Beef Cattle Research Station, Pasuruan, from January to March 2020. Materials of the study were Semen of 3 Filial Ongole Cattles, aged ± 5 years, with ± 700 kg body weight, mass motility ≥ 2+, and individual motility ≥ 70%. The method used was experimental using a Randomized Group Design with 3 treatment volume when sexing i.e. T0= 1 ml, T1= 1.5 ml dan T2= 2 ml with repetition 11 times. The repetition also functions as groups (blocks). The results showed that after sexing and cooling up to 5oC, the percentage of motility is T0:71.02+10.08 %; T1: 79.63+8,65 % and T2: 83.38+6.67 %. Progressive motility on T0: 47.68+8.71%; T1: 59.61+7.26 % and T2: 62.21+6.74%. Curvilinear Velocity (VCL) on T0: 50.9±7.73 µm/s, T1: 55.2±5.03 µm/s and T2: 53.2±5.97%. Straight-line Velocity (VSL) on T0: 20.8±8.19 µm/s; T1: 21.6±6.02 µm/s; T2: 22.1±5.77 µm/s. The Average Path Velocity (VAP) on T0: 30.2±7.82 µm/s; T1: 32.5±6.14 µm/s and T2 : 31.5±6.18 µm/s. Linearity (LIN) on T0: 40.2±12.26%; T1: 39.1±10.31%;T2: 39.8±7.25%. Straightness (STR) on T0: 67.2±11.20%; T1: 65.7±10.06%; T2: 67.2±7.92%. Wobble (WOB) on T0: 59±9.49%; T1: 58.8±8.63% and T2: 59.1±7.74%. The conclusion of the study is the motility and progressive motility of spermatozoa in samples with an initial volume of 2 ml is better than samples with an initial volume of 1 ml and 1.5 ml.

show abstract

“…The Percoll density gradient centrifugation method settled the X-bearing sperm cells in the lower layer at 60.75%, while the Y-bearing sperm cells were obtained at the upper (supernatant) [2]. The Percoll density gradient centrifugation shifted the ratio of X-and Y-bearing sperm cells to 28%:72%, respectively [36]. The Y-bearing sperm cells swim-up faster than the X-bearing sperm cells [3].…”

Section: Discussionmentioning

confidence: 99%

The enriched Y-bearing sperm combined with delayed fixed-time artificial insemination for obtaining male Simmental crossbred offspring

et al. 2022

View full text Add to dashboard Cite

Background and Aim: The production of male calf beef cattle is an agricultural innovation needed to increase the farm's productivity as a provider of meat sources. This study aimed to determine the sex ratio of the offspring of cows inseminated with Y-bearing sperm enriched by Percoll density gradient centrifugation and swim up, combined with delayed fixed-time artificial insemination (FTAI). Materials and Methods: Ejaculates of Simmental bulls were divided into four equal portions and grouped as T0 (control, non-sexed semen), T1 and T2 were sexed semen using Percoll density gradient centrifugation three and five levels, respectively, and T3 was sexed semen using swim-up. After the sex was sorted, the semen was diluted in a tris egg yolk extender, packaged in French mini-straws containing 50 million live sperm cells, and frozen. Pre-sexed, post-sexed, and post-thawed spermatozoa were evaluated based on progressive motility, viability, intact plasma membrane, and abnormality. The post-thawed semen of T0 was artificially inseminated to recipient cows at 12 h after onset of estrus (not delayed FTAI). Meanwhile, the delayed FTAI was conducted 18 20 h after onset of estrus using the T0, the best of T1 and T2, and the T3 post-thawed semen. Results: The Percoll density gradient centrifugation reduced motility, viability, and intact plasma membrane but increased sperm abnormalities. Meanwhile, the swim up process increased motility, viability, and intact plasma membrane of sperm cells but decreased sperm abnormalities. Post-thawed semen decreased motility, viability, and intact plasma membrane of sperm cells but increased sperm abnormalities. The sex ratio of the Simmental crossbred offspring was 96.08% and 100% in T1 and T3, respectively, compared to 48.25% and 67.39% in T0 not delayed and delayed FTAI, respectively. Conclusion: The Percoll density gradient centrifugation and swim-up methods are prospective for obtaining male offspring.

show abstract

The Motility and Ratio of X and Y Sperm Filial Ongole Cattle Using Different Sexed Semen Methods

Cited by 11 publications

References 8 publications

Relationship of motility and acrosome reaction on sexing sperm in Ongole Crossbred bull

Relationship of motility and acrosome reaction on sexing sperm in Ongole Crossbred bull

Karakter Motilitas Spermatozoa Hasil Sexing pada Sapi Peranakan Ongole dengan Volume Awal yang Berbeda

The enriched Y-bearing sperm combined with delayed fixed-time artificial insemination for obtaining male Simmental crossbred offspring

Contact Info

Product

Resources

About