Karakter Motilitas Spermatozoa Hasil Sexing pada Sapi Peranakan Ongole dengan Volume Awal yang Berbeda

Abstract

ABSTRAK. Sexing spermatozoa bertujuan untuk mengatur jenis kelamin sesuai harapan. Salah satu metode sexing adalah dengan menggunakan Sentrifugasi Gradien Densitas Percoll. Tujuan dari penelitian ini adalah untuk mengetahui berbagai parameter motilitas menggunakan Computer-assisted Sperm Analysis dan proporsi spermatozoa X dan Y menggunakan metode Sentrifugasi Gradien Densitas Percoll dengan volume awal yang berbeda. Penelitian dilakukan di Loka Penelitian Sapi Potong Grati, Pasuruan, pada bulan Januari sampai Maret 2020. Materi yang digunakan adalah Semen Sapi Peranakan Ongole berumur ±5 tahun dan bobot badan ±700 kg sebanyak 3 ekor, motilitas masa ≥ 2+ dan motilitas individu ≥ 70%. Metode yang digunakan adalah eksperimental menggunakan Rancangan Acak Kelompok (RAK) dengan 3 perlakuan volume saat sexing yaitu P1= 1 ml, P2= 1,5 ml dan P3= 2 ml dengan ulangan 11 kali. Ulangan juga berfungsi sebagai kelompok (block). Hasil penelitian menunjukkan bahwa setelah dilakukan sexing dan pendinginan hingga 5oC persentase motilitas adalah P1:71,02+10,08 %; P2: 79,63+8,65 % dan P3: 83,38+6,67 %, sedangkan motilitas progresif pada P1: 47,68+8,71%; P2: 59,61+7,26 % dan P3: 62,21+6,74%. Curvilinear Velocity (VCL) pada P1: 50,9±7,73 µm/s, P2: 55,2±5,03 µm/s dan P3: 53,2±5,97%. Straight-line Velocity (VSL) pada P1: 20,8±8,19 µm/s; P2: 21,6±6,02 µm/s; P3: 22,1±5,77 µm/s, sedangkan Average Path Velocity (VAP) pada P1: 30,2±7,82 µm/s; P2: 32,5±6,14 µm/s dan P3 : 31,5±6,18 µm/s. Linearity (LIN) pada P1: 40,2±12,26%;P2: 39,1±10,31%;P3: 39,8±7,25%. Straightness (STR) pada P1: 67,2±11,20%; P2: 65,7±10,06%;P3: 67,2±7,92% sedangkan Wobble (WOB) pada P1: 59±9,49%; P2: 58,8±8,63% dan P3: 59,1±7,74%. Kesimpulan dari penelitian adalah motilitas dan motilitas progresif spermatozoa pada sampel dengan volume awal 2 ml lebih baik dibandingkan dengan sampel dengan volume awal 1 ml dan 1,5 ml. (Characteristics of Sexing Spermatozoa Motility in Ongole Cattle with Different Initial Volume) ABSTRACT. Sperm sexing is a technique of sorting a specific type of sperm cell to fertilize the egg cell. One of the sexing methods that can be used for spermatozoa sexing is percoll gradient density centrifugation. This research aims to find out various motility parameters using Computer-assisted Sperm Analysis and the proportion of spermatozoa X and Y using density gradient centrifugation method percoll with different initial volume. The study was conducted at Grati Beef Cattle Research Station, Pasuruan, from January to March 2020. Materials of the study were Semen of 3 Filial Ongole Cattles, aged ± 5 years, with ± 700 kg body weight, mass motility ≥ 2+, and individual motility ≥ 70%. The method used was experimental using a Randomized Group Design with 3 treatment volume when sexing i.e. T0= 1 ml, T1= 1.5 ml dan T2= 2 ml with repetition 11 times. The repetition also functions as groups (blocks). The results showed that after sexing and cooling up to 5oC, the percentage of motility is T0:71.02+10.08 %; T1: 79.63+8,65 % and T2: 83.38+6.67 %. Progressive motility on T0: 47.68+8.71%; T1: 59.61+7.26 % and T2: 62.21+6.74%. Curvilinear Velocity (VCL) on T0: 50.9±7.73 µm/s, T1: 55.2±5.03 µm/s and T2: 53.2±5.97%. Straight-line Velocity (VSL) on T0: 20.8±8.19 µm/s; T1: 21.6±6.02 µm/s; T2: 22.1±5.77 µm/s. The Average Path Velocity (VAP) on T0: 30.2±7.82 µm/s; T1: 32.5±6.14 µm/s and T2 : 31.5±6.18 µm/s. Linearity (LIN) on T0: 40.2±12.26%; T1: 39.1±10.31%;T2: 39.8±7.25%. Straightness (STR) on T0: 67.2±11.20%; T1: 65.7±10.06%; T2: 67.2±7.92%. Wobble (WOB) on T0: 59±9.49%; T1: 58.8±8.63% and T2: 59.1±7.74%. The conclusion of the study is the motility and progressive motility of spermatozoa in samples with an initial volume of 2 ml is better than samples with an initial volume of 1 ml and 1.5 ml.

Analysis of Motility Characteristic of Pesisir Bulls Sexed Semen with Different Pre-Freezing Method Based on Computer Assisted Sperm Analyzer (CASA)

“…These result were obtained because management of frozen semen production did not affect spermatozoa biology. This results in this study is higher than the report of Mustofa et al (2020) whom report that the best VCL value on sexed semen of Ongole crossbreed was 55.2 μm/s. VCL is the velocity of spermatozoa on their pathway measured in μm/s.…”

Testing Motility Parameters of Post-Thawing Pesisir Bulls (Bos indicus) Semen with and without Sexing

“…VCL for spermatozoa Y (112.97μm/s) is significantly higher than that of spermatozoa without sexing (95.04μm/s) and spermatozoa X (93.95μm/s). The findings of this study indicate a higher value compared to the report by Mustofa et al (2020), who stated that the optimal VCL value for sexed semen of Ongole crossbreed was 55.2 μm/s. Similar to the values of DCL, DAP, and DSL, spermatozoa Y have higher velocity values compared to spermatozoa X.…”

Evaluation of Motility, Viability, and Integrity Plasma Membranes of Frozen Semen in Friesian Holstein with Storage Periods of 33, 30, 27, and 24 Years

“…According to Munazaroh et al, (2013) a decrease in spermatozoa viability can be caused by friction between spermatozoa and each other, temperature, storage time for cell metabolism, and availability of nutrients. According to Mustofa et al, (2021) one solution to increase the percentage of spermatozoa viability after thawing is to mix 0,1 mg/100 green tea extract into the dilute.…”

“…Spermatozoa with an intact plasma membrane is characterized by a spermatozoa tail that looks circular, whereas spermatozoa with a damaged plasma membrane are characterized by a tail that looks straight (Mustofa et al, 2021). The addition of HOS solution to semen makes the pressure on the spermatozoa cells become hyperosmotic compared to the outside of the cells.…”