Relationship of motility and acrosome reaction on sexing sperm in Ongole Crossbred bull

Abstract

Artificial insemination using sexing semen is expected to produce calves with the desired sex. One sexing sperm method is the percoll density gradient centrifugation method. This study aimed to determine the changes and the relationship between motility and acrosome reaction after sexing process using percoll density gradient centrifugation. The material used was semen of ±5 years old Ongole crossbred bull with a bodyweight of ±700 kg as many as three bulls with mass motility 2+ and individual motility 70%. The method used was to compare fresh semen with sexed semen after the cooling process. Parameters measured were motility characters using CASA analysis, which included motility parameters, progressive motility, capacitation, and no acrosome reaction. Statistical analysis used paired T-test to distinguish among fresh semen, after sexing and cooling process. In comparison, regression and correlation were used to analyze the relationship of capacitation and hyperactivation sperm with no acrosomal reaction with motility and progressive motility. The results showed that motility and progressive motility decreased after the sexing and cooling process. Meanwhile, the acrosomal reaction, capacitation, and hyperactivity increased.

The Quality and Proportion of Spermatozoa X and Y in Sexed Frozen Semen Separated with Percoll Density Gradient Centrifugation Method on Friesian Holstein Bull

“…Sexing frozen semen has passed various processes such as dilution, separation of spermatozoa, cooling, and freezing, all of which can damage the structure of the spermatozoa membrane and cause a decrease in quality. In this study, the acrosomal reaction on sexed semen in T1 and T2 was higher than in unsexed semen T0, and this result is in line with the research of (Yekti et al, 2022) that the process of sexing and cooling sperm also increased capacitation, acrosomal reactions, and hyperactivation.…”

“…The average spermatozoa that have not undergone capacitation obtained in this study are still relatively high, so the semen used is still suitable for AI. This is because the process of capacitation of spermatozoa should occur in the female reproductive tract in order be able to penetrate the oocyte (Yekti et al, 2022). The proportion of spermatozoa can confirm the success of the sexing method in separating spermatozoa.…”

“…Preparations can be directly observed using a fluorescence microscope with as many as 200 spermatozoa using 400x magnification. Preparations can also be put in a preparation box wrapped in aluminum foil and then stored in the freezer for later observation (Yekti et al, 2022).…”

Molecular Verification of Spermatozoa Sexing Method in Pesisir Cattle Bull Using Bovine Serum Albumin Column

“…Spermatozoa sexing is advantageous for male Pesisir cattle (Pesisir bulls) because it permits the provision of frozen semen, which can be used for controlling the sex of the desired calf according to required maintenance during breeding. However, the process may lead to changes in motility and viability, acrosome reaction acceleration, elevated spermatozoa capacitation, membrane damage, and fragmentation (Yekti et al 2022;Sallam et al 2023).…”