The Quality and Proportion of Spermatozoa X and Y in Sexed Frozen Semen Separated with Percoll Density Gradient Centrifugation Method on Friesian Holstein Bull

Yekti, Aulia Puspita Anugra; Rahayu, Sri; Ciptadi, Gatot; Susilawati, Trinil

doi:10.17582/journal.aavs/2023/11.3.371.378

Cited by 5 publications

(12 citation statements)

References 17 publications

(37 reference statements)

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“…Dead sperm cells absorbed the stain, while live sperm cells did not, and 200 sperm were examined in each sample, following the method outlined by [5]. Spermatozoa concentration was calculated using the Neubauer counting chamber, as described by [5] and [3]. The concentration was calculated using the formula provided by [3] as follows.…”

Section: Assessment Of Sperm Quality After Sexingmentioning

confidence: 99%

“…Spermatozoa concentration was calculated using the Neubauer counting chamber, as described by [5] and [3]. The concentration was calculated using the formula provided by [3] as follows.…”

Section: Assessment Of Sperm Quality After Sexingmentioning

confidence: 99%

“…Differences in the DNA mass of X and Y spermatozoa lead to variations in their weight and density [9]. Due to these differences, during centrifugation, X spermatozoa settle in the bottom layer, while Y spermatozoa remain in the top layer [3]. However, individual motility in both T0 and T1 decreased compared to the motility of fresh semen, suggesting that centrifugation impacts sperm motility negatively.…”

Section: Semen Quality Post Sexingmentioning

confidence: 99%

“…This disparity arises because spermatozoa in the upper and lower layers, subjected to sexing with five gradients, suffer membrane damage. [3] have added that the rotation during the centrifugation process causes internal spermatozoa collisions with tube walls and friction with the medium, leading to spermatozoa membrane damage. Such damage results in increased spermatozoa abnormalities [13].…”

Section: Semen Quality Post Sexingmentioning

confidence: 99%

“…The sexing process can cause membrane damage, leading to changes in sperm motility and viability. Moreover, recent research by [3] indicates that this method reduces motility and viability and decreases sperm concentration. This reduction occurs due to the intricate process involved: separation, gradient levels, centrifugation, dilution, and freezing.…”

Section: Introductionmentioning

confidence: 99%

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Quality and Proportion of Spermatozoa in the Percoll Density Gradient Centrifugation Sexing Method at Different Gradients using AndroMed® Diluent

Simbolon,

Pramudhita,

Rizqianti

et al. 2024

BIO Web Conf.

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The utilization of sexing technology is an effort made to improve the efficiency of livestock farming, which was created to predict the sex of the calves born so that it can be adjusted to the objectives of the farm. This study aims to analyze the quality and proportion of sexed spermatozoa using the percoll density centrifugation method at different gradients with AndroMed® diluent. The research material used was fresh semen from Belgian Blue Bull aged six years with a body weight of 600 kg and fresh semen quality motility ≥70%. This research is a laboratory experiment with two treatments and ten replicates. The treatments in the study were T0: Ten Gradient density percoll + AndroMed® and T1: Five Gradient density percoll + AndroMed®. The research analysis used a dependent t-test. The statistical analysis showed a significant difference (P<0.05) in treating ten gradients with five gradients in each layer regarding individual motility, viability, abnormality, and total motile spermatozoa. At the same time, in concentration, there was no difference (P>0.05) in the treatment of 10 gradients with five gradients. The average results on individual motility, abnormality, concentration, and total motile spermatozoa showed treatment of 10 gradients better than treatment of 5 gradients in each layer. At the same time, the variable viability showed that the gradient treatment is better than the five-gradient treatment. Proportion of spermatozoa in the Upper Layer T0 X (23.4%), Y (76.8%) and T1 X (16.9%), Y (83.1%). The proportion of Spermatozoa in the Bottom Layer was T0 X (84.1%), Y (15.9%), and T1 X (83%), Y (17%), respectively. In conclusion, sexing spermatozoa with the Percoll 10 and 5 Gradient Density Centrifugation method can separate X and Y spermatozoa.

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Section: Assessment Of Sperm Quality After Sexingmentioning

confidence: 99%

“…Spermatozoa concentration was calculated using the Neubauer counting chamber, as described by [5] and [3]. The concentration was calculated using the formula provided by [3] as follows.…”

Section: Assessment Of Sperm Quality After Sexingmentioning

confidence: 99%

Section: Semen Quality Post Sexingmentioning

confidence: 99%

Section: Semen Quality Post Sexingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Quality and Proportion of Spermatozoa in the Percoll Density Gradient Centrifugation Sexing Method at Different Gradients using AndroMed® Diluent

Simbolon,

Pramudhita,

Rizqianti

et al. 2024

BIO Web Conf.

Self Cite

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The Study of Artificial Insemination with Double Doses at Different Times in Friesian Holstein Crossbred

Susilawati¹,

Yekti²,

Firdaus³

et al. 2023

AAVS

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The reproductive performance of dairy cows in Indonesia still needs to be improved. The method of Double-dose AI can highly improve the success of AI. The materials used were 160 cows divided into 80 cows inseminated by a single dose and 80 cows inseminated with a double dose. There were two treatments in this research; T1 was AI single dose at the 8th hour after estrus onset, while T2 AI double dose at the 2nd and 8th hour after estrus onset. The Non-Return Rate (NRR 1) was observed on the next cycle after AI. If the cows showed the estrus sign, the insemination was repeated with the same treatment and then continued to observe NRR 2 on the next cycle. After two months, the pregnancy and AI failure were observed using ultrasound. A Chi Square was performed using SAS OnDemand for Academics (ODA, Cary, NC, USA). Moreover, probability values were calculated using the least significant different testing. The results showed that NRR-1 was 83.75% and 85%, while NRR-2 was 62.5% and 68.75% on T1 and T2, respectively. Furthermore, the failure of AI was mainly caused by repeat breeders, which were 28.75% on T1 and T2. It consists of 95.65% and 87.5% of normal ovaries, Corpus luteum persistent at 4.35% and 8.34%, and ovarian hypo function at 0% and 4.16% in T1 and T2, respectively. In conclusion, Artificial Insemination using the double doses method did not significantly improve the pregnancy rate of dairy cows.

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Hematological Profile and Leukocyte Differentiation of Madura Cattle and their Crosses at Different Sexes and Ages

Agustina¹,

Suyadi²,

Nugiartiningsih³

et al. 2023

AAVS

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This study analyses the Haematological profile, and leukocyte differentiation of Madura cattle and its crossbreeds of different sex and age. The results are expected to provide a better understanding of the condition of livestock raised by the community. Blood samples were obtained from 60 heads of Madura and Limura (Limousine x Madura) cattle, which were differentiated by sex and age. Blood samples were collected into tubes and immediately stored in a cold box at approximately 4°C. Data analysis was conducted at the

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The Quality and Proportion of Spermatozoa X and Y in Sexed Frozen Semen Separated with Percoll Density Gradient Centrifugation Method on Friesian Holstein Bull

Cited by 5 publications

References 17 publications

Quality and Proportion of Spermatozoa in the Percoll Density Gradient Centrifugation Sexing Method at Different Gradients using AndroMed® Diluent

Quality and Proportion of Spermatozoa in the Percoll Density Gradient Centrifugation Sexing Method at Different Gradients using AndroMed® Diluent

The Study of Artificial Insemination with Double Doses at Different Times in Friesian Holstein Crossbred

Hematological Profile and Leukocyte Differentiation of Madura Cattle and their Crosses at Different Sexes and Ages

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