Succesfull of Artificial Insemination by Using Chilled Semen on Brahman Cross Cows

Abstract

Successfull of artificial insemination by using frozen semen is relatively low, because of decreasing of sperm motility after frozen process. In this study, the chilled semen was used to increase the succesful of artificial insemination. The purpose of this study was to evaluate the successful of artficial insemination by using chilled semen on Brahman Cross cows. In this study the semen was diluted with two kinds of extender were CEP without BSA + egg white albumin and tris aminomethane without raffinose. The material used on this study were 45 brahman cross x cows wich were inseminated by chilled semen using CEP without albumin +egg white (T1) and 45 brahman cross X cows were inseminated with chilled semen using Tris Aminomethane Without raffinose (T2). The chilled semen have the individual motility at least 40% to be inseminated to the female recipient which were stored at 4°C. The parameter observed were non return rate (NRR) and conception rate (CR). The data was analyzed by using chi-square analysis and t-test analysis. T-test result showed that there were significant differences (P<0.01) between T2 and T1 on conception rate and NRR. Then chi square result showed that there were significant differences T1 (P<0.05) and T2 (P<0.01) on conception rate wichs is the CR for T1 Was 66,7%, while on T2 was 62,2 % where the expected pregnancy rate was 60%. it indicates that chilled semen both using tris aminomethane extender and CEP extender can be used to be inseminated to the cows . In conclusions, the results clearly showed that chilled semen can be successfully inseminated to the Brahman cross cows.

Benefits of Nori Fruit Extract (Morinda Citrifolia Linn) in Increasing the Storage of Spermatozoa Y Bali Cows Resulting from Sexing at a Temperature of 5oC

“…According to Rizal (2009), semen reservation can cause the death of spermatozoa, due to damage to the spermatozoa plasma membrane due to lipid/fat peroxidation and during the sperm sexing process this can result in damage to the plasma membrane. P2 and P3 treatment on day 7 and P2 can last until day 11 showing progressive motility ≥ 40% so that the semen can still be used for AI purposes, according to SNI standards that semen used for AI has at least progressive motility ≥ 40% (Yekti et al, 2018).…”

“…The implementation of AI using liquid semen shows a high pregnancy rate (Quan et al, 2016). AI using liquid semen is an alternative for storing semen in a refrigerator (temperature 2-4 o C) for areas where liquid N2 is not available (Yekti et al, 2018).…”

The effect of protected soybean meal as a protein supplement on blood metabolites of lactating dairy cows

“…Hasil uji semen beku yang digunakan pada penelitian ini memiliki kualitas Post Thawing Motility (PTM) pada P0 (semen beku non sexing) 36,00 ± 1,00 % sedangkan P1 (semen beku sexing) 31,40 ± 1,84%, Hasil penelitian Susilawati et al, (2014) (Yekti et al, 2018). Munculnya berahi ulang pasca IB ditunjukkan dengan gejalagejala seperti intensitas bersuara tinggi, vulva bengkak dan agak mengkilat, berlendir, induk gelisah dan jika didekati akan diam serta sebagian induk terjadi penurunan nafsu makan dan minum.…”

Integrity of Sperm Cell Bali Bull Preserved at 5°C Using a Diluent Formulation of Extracted Red Fruit and Coconut Water

“…The implementation of IB is expected to lead to better yields. The quality of semen produced by a male influence the success of IB (Yekti, et al, 2017;Indriani, et al, 2013). During cryopreservation, semen quality decreases due to cold shock thermal factors, mechanical formation of intracellular ice crystals, chemical (diluent components), and osmotic stress (osmotic pressure of the diluent).…”