Study on the Antibacterial Activity of Fruit Extracts of Klutuk Banana (Musa Balbisiana Colla) Against Shigella Dysenteriae Atcc 13313

Abstract

Objective: The purpose of this study is to determine the antibacterial activity of Klutuk Banana (Musa balbisiana colla) fruit extracts against Shigella dysenteriae ATCC 13313 and the amount of potassium to the discovery of anti-dysentery drug candidates.Methods: The simplisia of Klutuk banana fruit was extracted with ethanol using a maceration method. The phytochemical screening of ethanol extract was performed using standard procedures. Determination thin layer chromatography (TLC) profile of the extract was performed using a thin layer plate. The antibacterial activity was investigated using agar well diffusion technique. The minimum inhibitory concentrations (MIC) were determined by a serial microdilution method, whereas the minimum bactericidal concentration (MBC) was done by subculturing the MIC result onto agar medium. Potassium levels of the extract were carried out quantitatively using atomic absorption spectrophotometry.Results: The phytochemical analysis revealed the presence of flavonoids, polyphenols, tannins, monoterpenoid and sesquiterpenoids, quinones, and saponins. The TLC results prove the existence of flavonoids in the tested extract. The content of secondary metabolites that can act as an antibacterial, strengthen the antibacterial activity of ethanol extract of Klutuk banana against S. dysenteriae 13313 with MBC values in the range of 5-10%w/v. Potassium levels in the ethanol extract of Klutuk banana fruits contain potassium as much as 2.919% (29 190 ppm).Conclusion: It can be concluded that the ethanol extract of Klutuk banana fruits is more potent as antibacterial against S. dysenteriae than as potassium supplier in hypokalemia therapy.

Antibacterial Activity of Ethanolic Extract of Sangkareho (Callicarpa longifolia Lam.) against Staphylococcus epidermidis

“…The yield of the extract From the rendement calculation, the extraction of Sangkareho yields of 9.3% (Figure 1). Value yield is related to the number of secondary metabolites that successfully attracted when the extraction process (Kusuma et al, 2017).…”

“…The use of 96% ethanol solvents was due to its universal properties that capable of dissolving almost all types of secondary metabolites that have low molecular weight such as flavonoids, saponins, and alcohol; non-toxic and safe to use (Pratiwi et al, 2013;Azis et al, 2014;Arifianti et al, 2014). The percentage yields (w/w) of the extracts were calculated using the formula below (Kusuma et al, 2017):…”

Antibacterial Activity Test of Extracts and Fractions of Cassava Leaves (Manihot esculenta Crantz) against Clinical Isolates of Staphylococcus epidermidis and Propionibacterium acnes Causing Acne

“…. Determination of MIC and MBC fraction of the ethanol extract of cassava leaves against S. epidermidis and P. acnes clinical isolates was carried out by the microdilution method [29]. Columns 1-12 of the microdilution plate are filled with 100 μL MHB.…”

Formulation and Evaluation of Anti Acne Gel Containing Citrus Aurantifolia Fruit Juice Using Carbopol as Gelling Agent

“…The tested bacteria were scratched on the surface of slant agar, then incubated for 18-24 h at 37 ° C. Each of P. acnes and S. epidermidis colonies were taken using Ose, then suspended into sterile physiological NaCl. The bacterial turbidity was measured using a spectrophotometer at λ 530 nm, compared with a 0.5 Mc Farland solution [28].…”