Formulation and Evaluation of Anti Acne Gel Containing Citrus Aurantifolia Fruit Juice Using Carbopol as Gelling Agent

Abstract

Objective: The objective of this study was to design a product of anti-acne gel containing Citrus aurantifolia fruit juice as an effective antibacterial to treat acne caused by Propionibacterium acne and Staphylococcus epidermidis using carbopol as a gelling agent. Methods:The fresh juice of C. aurantifolia fruit was obtained by juicer and pasteurized for 30 min at 65-70 °C. The minimum inhibitory concentration (MIC) of the fruit juice was determined using the microdilution method. Then, carbopol in different concentration was incorporated in a gel base formula to obtain a stable gel base. The fresh juice in different formulas (F1, F2 and F3) was evaluated for 28 d. The color, pH and viscosity of each formula were observed. In addition, the antibacterial potency of each formula was analyzed using the agar diffusion method against both tested bacteria. Results:The citrus MIC values of both test bacteria showed different results, 20-40 % v/v for P. acne and 5-10 % v/v for S. epidermidis. The MIC values were converted into in vivo concentration and the resulted concentrations for each formula were 25, 50 and 75 % v/v. For supporting the formula, the most stable base gel was achieved using carbopol 1 % as the gelling agent. Among three formulas, the anti-acne gel formula containing 75 % fruit juice with carbopol 1% was the best formula based on the physical and microbiological parameter.Conclusion: Thus, it was concluded that the antiacne gel of fruit juice of C. aurantifolia with carbopol as a gelling agent could produce the effective and stable gel of anti-acne product.

Effect of Maltodextrin Ratio to Klutuk Banana Fruit Extract (Musa Balbisiana Colla) Combined With Its Pseudostem Extract on Anti-Dysentery Granule Performance and Effectivity

“…The measurement was carried out by inserting a hose into the standard solution with the smallest concentration to the highest concentration. The absorbance measurement results were recorded and calculated linear regression so that a straight line equation was obtained to see the linearity [31].…”

Enhancement of Skin Permeability of Econazole Nitrate Using Novel Flexisomal Nanocarriers by Implementing Quality by Design (Qbd) Approach

“…A weight of 0.5 kg was put on the upper glass plate for 5 min. The increase in the diameter due to spreading of gel sample was noted [35,37].…”

“…At each speed, dial reading on the viscometer was noted. Direct multiplication of the dial readings with factors given in the Brookfield viscometer catalogue gave the viscosity in centipoises [34][35][36][37].…”

“…From this solution, 1 ml was withdrawn and volume adjusted to 10 ml with methanol. The absorbance was taken at a λmax 264 nm by UV-Spectrophotometer [35][36][37].…”

“…Skin cut into small pieces further homogenized and the drug present in the skin was extracted with 50 ml methanol by means of sonication, after 24 h filtered through 0.45 mm membrane filter and then analyzed with help of UV-visible spectrophotometer at wavelength 264 nm in suitable dilutions [7,36,37].…”