2005
DOI: 10.1590/s0001-37652005000200008
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Abstract: Lymphocytes activated by mitogenic lectins display changes in transmembrane potential, an elevation in the cytoplasmic Ca 2+ concentrations, proliferation and/or activation induced cell death. Low concentrations of ouabain (an inhibitor of Na + ,K + -ATPase) suppress mitogen-induced proliferation and increases cell death. To understand the mechanisms involved, a number of parameters were analyzed using fluorescent probes and flow cytometry. The addition of 100nM ouabain to cultures of peripheral blood lymphocy… Show more

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Cited by 17 publications
(10 citation statements)
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“…This is in accordance with the cell death observed in the thymus and the activation of an apoptotic pathway. Furthermore, other authors have demonstrated that OUA enhances apoptosis of different cells, including peripheral blood lymphocytes (Esteves et al 2005), cortical neurons (Xiao et al 2002) and telencephalic cells (Brines and Robbins 1992).…”
Section: Discussionmentioning
confidence: 96%
“…This is in accordance with the cell death observed in the thymus and the activation of an apoptotic pathway. Furthermore, other authors have demonstrated that OUA enhances apoptosis of different cells, including peripheral blood lymphocytes (Esteves et al 2005), cortical neurons (Xiao et al 2002) and telencephalic cells (Brines and Robbins 1992).…”
Section: Discussionmentioning
confidence: 96%
“…Similar to observations in the CNS, the effects vary with cell type and the concentration used. Ouabain was shown to inhibit mitogen-induced lymphocyte proliferation [17] , to regulate the production of proinflammatory cytokines such as interleukin (IL)-1, IL-6 and tumor necrosis factor-␣ [18,19] , to enhance lipopolysaccharide-induced nitric oxide production [20] and to induce mitogen-activated cell apoptosis [21,22] . Furthermore, nanomolar concentrations of ouabain led to an increase in intracellular Ca 2+ as a result of mobilization of both intracellular and extracellular Ca 2+ pools [23], and this was shown to correlate with the expression of CD69, a tyrosine kinase related to cell activation [24] .…”
mentioning
confidence: 99%
“…A different view is put forward by other authors [191,192], according to them loss of cytosolic K + occurs downstream of mitochondrial depolarization and caspase activation, but they also consider this step crucial for the activation of endonucleases [192,193]. In our experience the loss of mitochondrial membrane potential was clearly evident only when PHA-activated cells were exposed to ouabain and appeared to be a late event [181].…”
Section: Effect Of Ouabain On Activation-induced Cell Death (Aicd)mentioning
confidence: 46%
“…Fas and FasL are expressed in PHA-activated lymphocytes exposed or not to ouabain, and we observed that the induction of the apoptotic process using anti-Fas as an agonist, led to an increased amount of apoptotic cells in PHA-activated cultures in the presence of ouabain [181]. In the T cell line Jurkat, ouabain potentiates Fas-induced apoptosis and the death program triggered by this molecule [182,183].…”
Section: Effect Of Ouabain On Activation-induced Cell Death (Aicd)mentioning
confidence: 97%