Determination of Genetic Diversity Using 15 Simple Sequence Repeats Markers in Long Term Selected Japanese Quail Lines

Karabağ, Kemal; Balcıoğlu, Murat Soner; Karlı, Taki; Alkan, Sezai

doi:10.5713/ajas.15.0940

Cited by 3 publications

(3 citation statements)

References 17 publications

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“…The entire population obtained a higher Na value than the Ne value in the LGS population with a Na value of 16 and Ne of 13, while the lowest value was obtained in the PS population with a Na value of 5 and a Ne value of 5 (Table 2). According to Karabağ et al (2016), genetic variation is more likely to be detected if the mean Ne is high, which is defined as the inverse of homozygosity. The values of Na and Ne obtained in the PS population are identical.…”

Section: Genetic Parametersmentioning

confidence: 99%

Implementation of 16S rRNA Gene for Fish and Shrimp Barcoding in Mangrove Ecosystems in North Sumatra and Aceh, Indonesia

Hairani

Amelia

Susetya

et al. 2023

JIPK

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Highlight Research The genetic diversity of fish and shrimp from mangrove habitats was relatively substantial, with a heterozygosity of 0.926 and the exception of Langsa samples. AMOVA revealed that the diversity among individuals in the populations were higher compared to those within individuals. DNA barcode in mangroves is useful in developing mangrove conservation and restoration initiatives Abstract Fish and shrimp are among the species that are vulnerable to high level of disturbance in mangrove ecosystem. This study aimed to investigate the implementation of 16S rRNA gene for fish and shrimp barcoding in mangrove ecosystems in North Sumatra and Aceh, Indonesia. 50 samples fresh fish and shrimp were collected from five mangrove ecosystems in North Sumatra, namely Belawan, Lubuk Kertang, Percut Sei Tuan, and Pulau Sembilan and Langsa of Aceh for DNA extraction process. The research was divided into four activities: sample collection, DNA isolation, PCR amplification, and data analysis. DNA was extracted using kit extraction (kit Reliaprep gDNA Tissue Miniprep System) and with PCR amplification. Genetic parameters were analyzed using Gen Alex 6.51 software and the relationship between sites was examined using the MVSP 3.22. The genetic diversity of fish and shrimp from mangrove habitats was relatively substantial, according to the findings, with a heterozygosity of 0.926, with the exception of Langsa samples, which were only identified in subclass A1. Genetic relationship between sites revealed that all samples clustered into two branches and were randomly dispersed within each site. This work confirmed the 16S rRNA gene worked for fish and shrimp barcoding in mangrove ecosystems, North Sumatra and Aceh, Indonesia and our findings are expected to be useful in developing mangrove conservation and restoration initiatives.

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Section: Genetic Parametersmentioning

confidence: 99%

Implementation of 16S rRNA Gene for Fish and Shrimp Barcoding in Mangrove Ecosystems in North Sumatra and Aceh, Indonesia

Hairani

Amelia

Susetya

et al. 2023

JIPK

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“…It is the opinion of Machado et al (2003), who states that if the value of H0 is lower than that of He, it indicates the degree of endogamy (marriage in groups). Karabag et al (2016) stated that high genetic diversity has an observed heterozygosity value of more than 0,5. Heterozygosity is a parameter used to measure the level of genetic diversity in a population based on allele frequencies at each locus (Wang et al, 2015).…”

Section: Heterozygositymentioning

confidence: 99%

Myostatin (MSTN) Gene Polymorphism Using PCR-RFLP Method In Kerinci Ducks

Al-Sobri¹,

Depison²,

Wiyanto³

2022

Jurnal Sain Peternakan Indonesia

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This study aims to determine body weight, body weight gain, and body measurements of male and female Kerinci ducks, as well as to determine the diversity of MSTN genes and the association of the MSTN gene on body weight, body weight gain, and body sizes in Kerinci ducks. The materials used were 96 Kerinci ducks and 96 Kerinci duck blood samples. Data analysis included: T-test, T2-hotelling, Principal Component Analysis (PCA), genotype frequency, allele, Hardy-Weinberg equilibrium, Heterozygosity, and PIC. The results showed that body weights at 2 and 3 months of age, body weight gain of 2-3 months and body sizes of 3 months old male Kerinci ducks were significantly different (P <0.05) higher than female Kerinci ducks. Body size markers of male and female Kerinci ducks were the length of the sternum, the length of the shank, and the circumference of the shank, while the shape identifier was the length of the wings. The MSTN|MboI gene in Kerinci ducks was polymorphic. The population of Kerinci ducks was in Hardy-Weinberg equilibrium (P<0.05). Kerinci duck population heterozygosity showed H0<He. The MSTN|MboI gene was associated with BB, PBB, and body measurements of male and female Kerinci ducks, and the best genotype was (-/-).

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“…These findings indicate that the population is diverse. High genetic diversity has heterozygosity values of more than 0.50 (Karabağ et al 2016). The results of genotype frequency, allele frequency, heterozygosity, and chi-square analysis (χ 2 ) of the prolactin gene at various locus are presented in Table 2.…”

Section: Genotype Frequency Allele Frequency and Prolactin Gene Heterozigositymentioning

confidence: 99%

Novel Mutation of Exon 5 Prolactin Gene in IPB-D1 Chicken

Rohmah

Darwati

Ulupi

et al. 2020

JITV

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<p class="JITV-ReferenceIsi">The prolactin gene (PRL) is a gene that controls the incubation and egg production in laying chickens. The nature of incubation will reduce egg production and disrupt the reproductive system in local chickens. The purpose of this study was to identify the polymorphism of prolactin genes in IPB-D1 chickens using the direct sequencing method. The polymorphism of the exon 5 prolactin gene was carried out on 46 samples of IPB-D1 chicken DNA which was a collection of the Division of Animal Genetics and Breeding, Faculty of Animal Science IPB. DNA sequences as a reference for designing exon 5 primers were obtained from the National Center for Biotechnology Information (NCBI) with the GenBank access code: AF288765.2. DNA extraction was carried out using the phenol-chloroform technique. DNA amplification resulted in a PCR product with a size of 557 bp. In this study, the genotype frequency, allele frequency, heterozygosity value and Hardy-Weinberg equilibrium were calculated. The results of the study found 5 SNPs in exon 5, namely g.7823A>G, g.7835A>G, g.7886T>A, g.8052T>C, and g.8069T>C. All SNPs are polymorphic and in Hardy-Weinberg equilibrium except g.8052T>C. The g.7823A>G, g.7835A>G, g.8052T>C SNPs are synonymous mutations that do not change amino acids, while the g.7886T>A and g.8069T>C SNPs are non-synonymous that change amino acids. Both g.7886T>A and g.8069T>C SNPs are potential as a marker assisted selection for the characteristics of egg production in IPB-D1 chickens.</p>

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Determination of Genetic Diversity Using 15 Simple Sequence Repeats Markers in Long Term Selected Japanese Quail Lines

Cited by 3 publications

References 17 publications

Implementation of 16S rRNA Gene for Fish and Shrimp Barcoding in Mangrove Ecosystems in North Sumatra and Aceh, Indonesia

Implementation of 16S rRNA Gene for Fish and Shrimp Barcoding in Mangrove Ecosystems in North Sumatra and Aceh, Indonesia

Myostatin (MSTN) Gene Polymorphism Using PCR-RFLP Method In Kerinci Ducks

Novel Mutation of Exon 5 Prolactin Gene in IPB-D1 Chicken

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