Novel Mutation of Exon 5 Prolactin Gene in IPB-D1 Chicken

Abstract

<p class="JITV-ReferenceIsi">The prolactin gene (PRL) is a gene that controls the incubation and egg production in laying chickens. The nature of incubation will reduce egg production and disrupt the reproductive system in local chickens. The purpose of this study was to identify the polymorphism of prolactin genes in IPB-D1 chickens using the direct sequencing method. The polymorphism of the exon 5 prolactin gene was carried out on 46 samples of IPB-D1 chicken DNA which was a collection of the Division of Animal Genetics and Breeding, Faculty of Animal Science IPB. DNA sequences as a reference for designing exon 5 primers were obtained from the National Center for Biotechnology Information (NCBI) with the GenBank access code: AF288765.2. DNA extraction was carried out using the phenol-chloroform technique. DNA amplification resulted in a PCR product with a size of 557 bp. In this study, the genotype frequency, allele frequency, heterozygosity value and Hardy-Weinberg equilibrium were calculated. The results of the study found 5 SNPs in exon 5, namely g.7823A>G, g.7835A>G, g.7886T>A, g.8052T>C, and g.8069T>C. All SNPs are polymorphic and in Hardy-Weinberg equilibrium except g.8052T>C. The g.7823A>G, g.7835A>G, g.8052T>C SNPs are synonymous mutations that do not change amino acids, while the g.7886T>A and g.8069T>C SNPs are non-synonymous that change amino acids. Both g.7886T>A and g.8069T>C SNPs are potential as a marker assisted selection for the characteristics of egg production in IPB-D1 chickens.</p>

Revisit the Development of Native Indonesian Chickens from Red Jungle Fowls (Gallus gallus bankiva) to Commercial Chickens

“…Kesimpulan dari penelitian tersebut menunjukkan bahwa ayam Kampung hasil seleksi pada generasi G3 mempunyai pola pita L meningkat sebanyak 31,25% dibandingkan dengan generasi G0. Dengan demikian gen promotor prolaktin dapat digunakan sebagai gen kandidat untuk sifat mengeram dan dapat digunakan sebagai marker genetik untuk mempercepat seleksi (Rohmah et al 2020).…”

“…Ayam lokal mempunyai beberapa keistimewaan dibandingkan dengan unggas lainnnya, antara lain adalah dagingnya mempunyai cita rasa yang gurih dan spesifik serta dapat hidup dan berkembang dengan pemeliharaan ekstensif tradisional, yaitu pada siang hari dibiarkan hidup berkeliaran dengan pemberian pakan tambahan berupa limbah pertanian dan sisa dapur, sedangkan pada malam hari dibiarkan tidur pada pohon atau atap rumah penduduk (Jafendi & Sidadolog 2007). Kadar lemak daging ayam lokal lebih rendah dibandingkan dengan ayam ras (Tamzil 2017), relatif tahan terhadap serangan penyakit Flu Burung (Rohmah et al 2020), serta relatif tahan dipelihara di daerah panas (Tamzil et al 2013b, Tamzil et al 2014. Ayam lokal mempunyai beberapa kelemahan yaitu mempunyai laju pertumbuhan lambat dan tingkat produksi telur rendah (Jafendi & Sidadolog 2007), sehingga sulit dikembangkan secara massal.…”