Behavior of Chromed Catgut and Polyglecaprone 25 Sutures in the Urinary Bladder of Rats, With Special Reference to Stone Formation

Biondo-Simões, Maria de Lourdes Pessole; Collaço, Luis Martins; Veronese, Cristina; Ribas, Marlene Miranda; Flores, Silvana Negrelli

doi:10.1590/s0102-86501998000100004

Cited by 8 publications

(3 citation statements)

References 5 publications

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“…O exame histopatológico qualitativo e quantitativo realizado com equipamento de captação de imagem e tratamento digital contribuiu para eliminar o fator subjetivo da avaliação pelo patologista. Alguma outras técnica têm sido utilizadas 15,16,17,18 .…”

Section: Discussionunclassified

Ação do fator de crescimento de fibroblasto básico na cicatrização da aponeurose abdominal de ratos

Medeiros¹,

Filho²,

Rocha³

et al. 2003

Acta Cir. Bras.

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OBJETIVO: Trabalho realizado em ratos com o objetivo de estudar o efeito do Fator de Crescimento de Fibroblastos básico (FCFb) na cicatrização da aponeurose abdominal. MÉTODOS: Foram usados 20 ratos Wistar separados aleatoriamente em 2 grupos iguais. Os animais foram anestesiados com pentobarbital sódico na dose de 20 mg/Kg por via intraperitoneal e submetidos a laparotomia mediana de 4 cm, cuja camada aponeurótica foi suturada com mononylon 5-0. No grupo I foi aplicada a dose de 5mg de FCFb sobre a sutura da aponeurose. No grupo II (controle) foi aplicada solução salina 0,9% sobre a linha se sutura. Após observação por 7 dias os animais foram mortos com superdose de anestésico. A camada aponeurótica com 1,5 cm de largura foi submetida a teste de resistência à tensão empregando a Máquina de Ensaios EMIC MF500. Biópsias das zonas de sutura foram processadas e coradas com HE e o tricômico de Masson. Os achados histopatológicos foram quantificados através de sistema digital (Image pro-plus) de captura e processamento de imagens. Os dados obtidos foram analisados pelo teste T com significância 0,05. RESULTADOS: Nos animais do grupo I (experimental) a zona de sutura da camada aponeurótica suportou a carga de 1.103± 103,39gf. A quantificação dos dados histopatológicos desse grupo atingiu a densidade média 226± 29,32. No grupo II (controle) a carga suportada pela zona de sutura foi de 791,1± 92,77 gf. Quando foram comparadas as médias das resistências à tensão dos dois grupos, observou-se uma diferença significante (p<0,01). O exame histopatológico das lâminas desse grupo relevou densidade média 114,1± 17,01, correspondendo a uma diferença significante quando comparadas as médias dos dois grupos (p<0,01). CONCLUSÃO: Os dados permitem concluir que o FCFb contribuiu para aumentar a resistência da aponeurose suturada e para melhorar os parâmetros histopatológicos da cicatrização.
OBJECTIVE: As little is known about the use of basic Fibroblast Growth Factor (FCFb) in the healing of abdominal aponeurosis, an experimental work was done to study it. METHODS: Twenty Wistar rats randomly separated in two groups were used. They were anesthetised with pentobarbital 20 mg/Kg intraperitonealy, submited to a 4cm median laparotomy and the aponeurosis was sutured with nylon 5-0. In group I 5mg of FCFb was applied over the sutured aponeurosis end the group II received saline 0,9% over it. After seven days the rats were killed by an overdose of anesthetic. A transversal 1,5 cm wideness sample of aponeurosis was submited to tensil strenght test, using the assay device EMIC MF500. Biopsies from the sutured tissue were processed and colored by HE and Masson trichromic. The histopatologic data were quantitated and transformed into mean density by the digital equipment Image Pro-plus. The Student t test was used and the differences were significant when p<0,05. RESULTS: In group I the the mean tensil strength was 1.103± 103,39gf and the mean density of histopatologic data was 226± 29,32. In group I...

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Section: Discussionunclassified

Ação do fator de crescimento de fibroblasto básico na cicatrização da aponeurose abdominal de ratos

Medeiros¹,

Filho²,

Rocha³

et al. 2003

Acta Cir. Bras.

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“…All the suture materials are considered as a foreign bodies when they are used inside the urinary tract. (Case, Glenn and Postlethwait 1976;Bondo-Simoes, Collaco, Veronese, Ribas and Flores, 1998).…”

Section: Discussionmentioning

confidence: 99%

Closure of Cystotomy in Dogs Using Different Types of Suture Materials and Tissue Adhesive

Nassef

Ali

Nafady

et al. 2010

Assiut Veterinary Medical Journal

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Experimental ventral cystotomy wounds were performed on 72 adult dogs of different sex and age. Cystotomy wound was closed by chromic catgut, polyglycolic acid, vicryl, Polysorb, silk and methyl-2-Cyanoacrylate super glue. Animals were sacrificed at one, two and three weeks postoperatively, and site of operation were examined grossly, microscopically and by scanning electron microscopy. The results of this study reconfirms that catgut is the best thread used for suturing of the urinary bladder incisions. Polyglycolic acid, vicryl and Polysorb were hydrolyzed and they are not sufficient for suitable time for healing of wound. Silk not absorbed and can cause the nidus for urinary calculi. Non absorbable tissue adhesives were unsuitable for urinary bladder closure, because there is no healing at the site of operation and lead to deformity and shrinkage of the bladder wound.

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“…A análise histológica tem sido usada no estudo da cicatrização em cirurgias experimentais por diversos autores 16,17,18 . Neste estudo, as lâminas foram coradas pela técnica da hematoxilina-eosina por ser método de coloração simples, barato e eficaz para o estudo dos elementos celulares envolvidos no processo de cicatrização 12 .…”

Section: Discussionunclassified

Efeito do extrato de Passiflora edulis (maracujá) na cicatrização de bexiga em ratos: estudo morfológico

et al. 2006

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Efeito do extrato de ABSTRACT Purpose:To evaluate the effects of hydroalcoholic extract of Passiflora edulis leaves in the healing of urinary bladder in rats from histological aspects. Methods: Forty Wistar male rats were submitted to a longitudinal incision of the bladder followed by a stetching in only one level. After this common procedure, animals were divided at random two groups: Passiflora and Control. In the Passiflora group the only dosage used was administered by intraperitonial injection of hydoalcoholic extract of Passiflora edulis leaves while in the Control group distilled water was injected. Each subgroup was then divided in two subgroups according to the death of these animals: Control, three and seven days, Passiflora, three and seven days. After the death of these animals, an inventory of the abdominal cavity was performed and the bladder was removed. A comparative analysis was done between the two groups with microscopic evaluation of the healing. There was less acute inflammation (p=0.008), greater colagenous formation (p=0.001) and greater capillary neo-formation (p=0.000) in the third day Passiflora subgroup when compared to the Control subgroup of the third day. Results: There was less acute inflammation (p=0.001), greater fibroblastic proliferation (p=0.011) and greater colagenous formation (p=0.001) in the Passiflora subgroup of seventh day when compared with the Control seventh day subgroup. Conclusion: The use of Passiflora edulis leaves extract resulted in less acute inflammation, greater fibroblastic proliferation, colagenous formation and capillary neo-formation on rats bladder wound healing. Key words: Passiflora edulis. Wound Healing. Bladder. Rats. IntroduçãoA cicatrização é um processo que visa limitar os danos e restabelecer a integridade e a função dos tecidos afetados 1 .Os avanços da biologia celular e molecular têm permitido o estudo e ampliado o conhecimento das etapas da cicatrização. Sabe-se que os tecidos cicatrizam-se de maneira

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Behavior of Chromed Catgut and Polyglecaprone 25 Sutures in the Urinary Bladder of Rats, With Special Reference to Stone Formation

Cited by 8 publications

References 5 publications

Ação do fator de crescimento de fibroblasto básico na cicatrização da aponeurose abdominal de ratos

Ação do fator de crescimento de fibroblasto básico na cicatrização da aponeurose abdominal de ratos

Closure of Cystotomy in Dogs Using Different Types of Suture Materials and Tissue Adhesive

Efeito do extrato de Passiflora edulis (maracujá) na cicatrização de bexiga em ratos: estudo morfológico

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