Ação do fator de crescimento de fibroblasto básico na cicatrização da aponeurose abdominal de ratos

Abstract

OBJETIVO: Trabalho realizado em ratos com o objetivo de estudar o efeito do Fator de Crescimento de Fibroblastos básico (FCFb) na cicatrização da aponeurose abdominal. MÉTODOS: Foram usados 20 ratos Wistar separados aleatoriamente em 2 grupos iguais. Os animais foram anestesiados com pentobarbital sódico na dose de 20 mg/Kg por via intraperitoneal e submetidos a laparotomia mediana de 4 cm, cuja camada aponeurótica foi suturada com mononylon 5-0. No grupo I foi aplicada a dose de 5mg de FCFb sobre a sutura da aponeurose. No grupo II (controle) foi aplicada solução salina 0,9% sobre a linha se sutura. Após observação por 7 dias os animais foram mortos com superdose de anestésico. A camada aponeurótica com 1,5 cm de largura foi submetida a teste de resistência à tensão empregando a Máquina de Ensaios EMIC MF500. Biópsias das zonas de sutura foram processadas e coradas com HE e o tricômico de Masson. Os achados histopatológicos foram quantificados através de sistema digital (Image pro-plus) de captura e processamento de imagens. Os dados obtidos foram analisados pelo teste T com significância 0,05. RESULTADOS: Nos animais do grupo I (experimental) a zona de sutura da camada aponeurótica suportou a carga de 1.103&plusmn; 103,39gf. A quantificação dos dados histopatológicos desse grupo atingiu a densidade média 226&plusmn; 29,32. No grupo II (controle) a carga suportada pela zona de sutura foi de 791,1&plusmn; 92,77 gf. Quando foram comparadas as médias das resistências à tensão dos dois grupos, observou-se uma diferença significante (p<0,01). O exame histopatológico das lâminas desse grupo relevou densidade média 114,1&plusmn; 17,01, correspondendo a uma diferença significante quando comparadas as médias dos dois grupos (p<0,01). CONCLUSÃO: Os dados permitem concluir que o FCFb contribuiu para aumentar a resistência da aponeurose suturada e para melhorar os parâmetros histopatológicos da cicatrização.<br>OBJECTIVE: As little is known about the use of basic Fibroblast Growth Factor (FCFb) in the healing of abdominal aponeurosis, an experimental work was done to study it. METHODS: Twenty Wistar rats randomly separated in two groups were used. They were anesthetised with pentobarbital 20 mg/Kg intraperitonealy, submited to a 4cm median laparotomy and the aponeurosis was sutured with nylon 5-0. In group I 5mg of FCFb was applied over the sutured aponeurosis end the group II received saline 0,9% over it. After seven days the rats were killed by an overdose of anesthetic. A transversal 1,5 cm wideness sample of aponeurosis was submited to tensil strenght test, using the assay device EMIC MF500. Biopsies from the sutured tissue were processed and colored by HE and Masson trichromic. The histopatologic data were quantitated and transformed into mean density by the digital equipment Image Pro-plus. The Student t test was used and the differences were significant when p<0,05. RESULTS: In group I the the mean tensil strength was 1.103&plusmn; 103,39gf and the mean density of histopatologic data was 226&plusmn; 29,32. In group I...

Effects of the basic fibroblast growth factor and its anti-factor in the healing and collagen maturation of infected skin wound

“…The tensil strength of operative wounds depends on some factors, such as the collagen level and the thickness and organization of their fibers 1 . It was demonstrated in the present work that the topical application of FGFß stimulated the deposition of types I and III collagen as observed in other studies 14,15 . The concomitant use of FGFß and anti-FGFß antibody in open infected and clean wounds inhibited the collagen synthesis.…”

“…The application of FCFß increased the global synthesis of the collagen, in higher intensity the type I than type III collagen. This finding must have contributed strongly to the maturation of collagen corroborated by several studies 2,3,4,7,12,14,15,36,37,38,39,40,41 .…”

“…The authors observed reduction in healing time and significant deposition of collagen in wounds 21,24 . Medeiros et al observed that the topical application of a single dose FGFß in sutured skin wounds of rats stimulated the lymphocytes and fibroblasts proliferation, increased the collagen synthesis and tensil strength of wounds 14 . However, Stenberg et al, using a similar experimental design of the present work, demonstrated that FGFß enhances infected wounds, but does not increase tensil strength 10 .…”

“…In experiments with topical application of FGFß a significant inhibition of the open wounds contraction was observed 12,13 . Medeiros et al, as well as McGee et al, observed that topical application of FGFß in incisional wounds increased significantly the density of collagen and the tensil strength in rats o submitted to infection, steroids use, radiotherapy and imunossupressors 14,15 . Several studies have demonstrated that FGFß acts in the extracellular matrix stimulating angiogenesis, fibroblast migration, angiogenesis and fibrogenesis, enhancing the tensil strength and reducing the time for infected wounds scaring 3,4,5,6,7,8,9,10 .…”