Avaliação de bacterina e Lactobacillus plantarum frente à infecção experimental por Vibrio harveyi em pós-larvas de Litopenaeus vannamei

-Bacillus spp. have been used against diseases caused by bacteria that affect cultured shrimp, providing beneficial effects on the host shrimps by altering their microbial community, and improving zootechnical indexes. The objective of this work was to evaluate the effects of a diet supplemented with Bacillus cereus-a bacterium with probiotic potential-on post-larvae Litopenaeus vannamei shrimps grown in laboratory. The experiment lasted for fifteen days and consisted of six treatments-control (T 1 ), probiotic (T 2 ), Vibrio parahaemolyticus (VP) (T 3 ), probiotic and VP (T 4 ), V. alginolyticus (VA) (T 5 ), and probiotic and VA (T 6 ). The survival rate, weight gain, colonization capacity of the probiotic bacteria, pathogen count, and histopathological lesions were evaluated. There was no significant difference (p≥0.05) in survival between treatments. The groups with pathogens and without probiotics presented lower weight gain. The result of the Bacillus cereus count in the treatments T 2 , T 4 and T 6 were significantly different (p<0.05), the probiotic bacteria were more aggressive in competing for space and nutrients when compared to V. parahaemolyticus than when compared to V. alginolyticus. Animals fed with the probiotic presented lower counts of these pathogens than those fed without the probiotic (p<0.05). No histopathological lesions were found in the organs and tissues of the shrimps. Bacillus cereus showed a high colonizing capacity in post-larvae shrimps, causing a significant reduction of pathogens, probably by secreting antimicrobial substances and the competitive exclusion, which justifies their use as probiotic bacteria.Keywords: Litopenaeus vannamei. Antimicrobial. Bacillus cereus. Vibriosis. AVALIAÇÃO DE POTENCIAL PROBIÓTICO FRENTE À INFECÇÃO EXPERIMENTAL POR VÍBRIOS EM PÓS-LARVAS DE CAMARÃO MARINHORESUMO -Bacillus spp. têm sido utilizados contra bacterioses que acometem camarão marinho cultivado, proporcionando efeito benéfico sobre o hospedeiro, alterando a comunidade microbiana intestinal e melhorando índices zootécnicos. O objetivo deste trabalho foi avaliar os efeitos de uma dieta suplementada com Bacillus cereus, bactéria com potencial probiótico em pós-larvas de Litopenaeus vannamei cultivados em laboratório. O experimento teve duração de quinze dias e consistiu de seis tratamentos: T 1 controle; T 2 somente com probiótico; T 3 com Vibrio parahaemolyticus (VP); T 4 com probiótico e com VP; T 5 com V. alginolyticus (VA); e T 6 com probiótico e com VA. Foram avaliados a sobrevivência, o ganho de peso, a capacidade de colonização das bactérias probióticas, contagem de patógenos e lesões histopatológicas. Não foi verificada diferença significativa (P≥0,05) entre os tratamentos na sobrevivência. Os grupos desafiados com patógenos e sem uso de probióticos foram os que apresentaram menor ganho de peso. Na contagem de Bacillus cereus, houve diferença significativa (P<0,05) entre os tratamentos T 2, T 4 e T 6 , observando-se que o probiótico competiu melhor por espaço e nutrientes quand...

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“…, as recommended by Buglione et al (2008). The experiment was completed seven days after this addition.…”

Section: Experimental Designmentioning

confidence: 99%

PROBIOTIC POTENTIAL OF Bacillus cereus AGAINST Vibrio spp. IN POST-LARVAE SHRIMPS

et al. 2018

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“…The L. plantarum strain used in the present study has already demonstrated its probiotic potential to shrimp (L. vannamei), increasing survival after experimental infections against Vibrio spp. in post-larvae (BUGLIONE et al, 2008) and juveniles (VIEIRA et al, 2008(VIEIRA et al, , 2010, as well as shrimp reared on commercial farms (VIEIRA et al, 2016), increasing the digestibility of diets (BUGLIONE-NETO et al, 2013) and improving food conversion (VIEIRA et al, 2016), justifying its application regardless of the protein source used.…”

Section: Discussionmentioning

confidence: 99%

EFEITO DA FONTE DE PROTEÍNA E PROBIOTICO SOBRE TRATO INTESTINAL DO CAMARÃO BRANCO PACÍFICO Litopenaeus vannamei

et al. 2018

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This study aimed to evaluate the viability of supplementing two diets for the shrimp Litopenaeus vannamei with Lactobacillus plantarum. One using fish meal as a protein source and another using soy protein concentrate, as well as the effect of these formulations on shrimp intestinal microbiota. To assay probiotic viability in the formulated diets, the number of CFU g-1 was observed weekly over the course of four weeks. The viability of Lactobacillus plantarum in relation to the physical parameters of the diets, including stability, buoyancy, and expansion, was quantified. The effect of the diets on microbiota and intestinal tract morphology was determined by performing a 2x2 factorial experiment (two diets, with or without supplementation) in triplicate, totaling 12 experimental units, with five animals per unit, fed with 3.5% of biomass for 17 days. The concentration of lactic acid bacteria decreased over time, irrespective of protein source. The diet with fishmeal as a protein source, independent of probiotic supplementation, presented good stability and did not disintegrate after four hours. In contrast, the diet with soy protein concentrate, supplemented or not, disintegrated between 2.5 and 3 hours, presenting low stability. All diets presented 0% buoyancy. The expansion rate was higher in diets with soy protein concentrate, but without the influence probiotic supplementation or interaction between the factors. In the in vivo assay, both supplemented diets showed greater total heterotrophic bacteria count than without probiotic; however, no difference in count was noted in diets with different protein source. Lactic acid bacteria were only observed in the shrimp fed diets supplemented with probiotic. Histology of the intestinal tract showed that all intestines had intact, well-developed and well-organized cells, irrespective of diet. Thus, L. plantarum, when combined with different protein sources, produced similar effects on the structure and microbiota of the marine shrimp Litopenaeus vannamei.

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Enterobacteria and Vibrio from Macrobrachium amazonicum prawn farming in Fortaleza, Ceará, Brazil

Castelo-Branco

Sales

Brilhante

et al. 2016

Asian Pacific Journal of Tropical Medicine

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Enterobacteria do not seem to be the most important pathogens associated with M. amazonicum farming, whereas the recovery of Vibrio spp. from larviculture, with emphasis on Vibrio cholerae and V. vulnificus, deserves special attention due to their role as potentially zoonotic aquaculture-associated pathogens. Furthermore, the intermediate susceptibility of V. vulnificus to ampicillin reflects the importance of monitoring drug use in prawn farming.

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Avaliação de bacterina e Lactobacillus plantarum frente à infecção experimental por Vibrio harveyi em pós-larvas de Litopenaeus vannamei

Cited by 3 publications

References 16 publications

PROBIOTIC POTENTIAL OF Bacillus cereus AGAINST Vibrio spp. IN POST-LARVAE SHRIMPS

PROBIOTIC POTENTIAL OF Bacillus cereus AGAINST Vibrio spp. IN POST-LARVAE SHRIMPS

EFEITO DA FONTE DE PROTEÍNA E PROBIOTICO SOBRE TRATO INTESTINAL DO CAMARÃO BRANCO PACÍFICO Litopenaeus vannamei

Enterobacteria and Vibrio from Macrobrachium amazonicum prawn farming in Fortaleza, Ceará, Brazil

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