Enterobacteria and Vibrio from Macrobrachium amazonicum prawn farming in Fortaleza, Ceará, Brazil

Castelo-Branco, Débora de Souza Collares Maia; Sales, Juscelino Chaves; Brilhante, Raimunda Sâmia Nogueira; Guedes, Gláucia Morgana de Melo; Ponte, Yago Brito de; Sampaio, Célia Maria de Souza; Bandeira, Tereza de Jesus Pinheiro Gomes; Moreira, José Luciano Bezerra; Alencar, Lucas Pereira de; Paiva, Manoel de Araújo Neto; Cordeiro, Rossana de Aguiar; Monteiro, André Jalles; Pereira-Neto, Waldemiro de Aquino; Sidrim, José Júlio Costa; Rocha, Marcos Fábio Gadelha

doi:10.1016/j.apjtm.2015.12.006

Cited by 3 publications

(2 citation statements)

References 20 publications

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“…Dentre as espécies de Vibrio relatadas em M. amazonicum já foram citadas V. mimicus, V. vulnificus e V. cholerae. Estas espécies foram encontradas tanto no trato digestório, quanto na água da larvicultura, gerando preocupação sobre a presença de patógenos no ciclo produtivo (Brilhante et al, 2015;Castelo-Branco et al, 2016).…”

Section: Vibrioses Na Carcinicultura E O Potencial Antimicrobiano Da ...unclassified

Engenharia de Alimentos: tópicos físicos, químicos e biológicos - Volume 1

2024

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Esta obra constituiu-se a partir de um processo colaborativo entre professores, estudantes e pesquisadores que se destacaram e qualificaram as discussões neste espaço formativo. Resulta, também, de movimentos interinstitucionais e de ações de incentivo à pesquisa que congregam pesquisadores das mais diversas áreas do conhecimento e de diferentes Instituições de Educação Superior públicas e privadas de abrangência nacional e internacional. Tem como objetivo integrar ações interinstitucionais nacionais e internacionais com redes de pesquisa que tenham a finalidade de fomentar a formação continuada dos profissionais da educação, por meio da produção e socialização de conhecimentos das diversas áreas do Saberes. Agradecemos aos autores pelo empenho, disponibilidade e dedicação para o desenvolvimento e conclusão dessa obra. Esperamos também que esta obra sirva de instrumento didático-pedagógico para estudantes, professores dos diversos níveis de ensino em seus trabalhos e demais interessados pela temática.

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Section: Vibrioses Na Carcinicultura E O Potencial Antimicrobiano Da ...unclassified

Engenharia de Alimentos: tópicos físicos, químicos e biológicos - Volume 1

2024

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“…[4], Ctenopharyngodon idellus [5], and Trachinotus ovatus [6] from Asia and Europe since the 1970s [7]. In Brazil, there have been reports of isolation and molecular identification of V. vulnificus from aquatic environments [8,9], human blood (patient who died of septicemia after ingestion of seafood) [10], wild and farmed healthy shellfish [11], and wild captured fish such as Paralichthys orbignyanus and Micropogonias furnieri [12]. In addition, there are reports of presumptive identification of V. vulnificus in sea mammals [13] and sea birds [14] in the country; however, these isolates have not been confirmed at the species level.…”

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confidence: 99%

First Report of Vibrio vulnificus Outbreak in Farm-Raised Sorubim (Pseudoplatystoma sp.) from Brazil

Janampa-Sarmiento,

Reis,

Egger

et al. 2024

Fishes

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Vibrio vulnificus is an opportunistic pathogen in humans and exhibits pathogenic behavior in several aquaculture fish species. To date, in Brazil, there are no reports of V. vulnificus outbreaks in farmed fish. However, in 2019, sorubim (Pseudoplatystoma sp.) in the grow-out phase with clinical signs and cumulative mortality of 40% was registered. We aimed to identify and characterize the etiological agents of this outbreak. Seven moribund fish were sampled for bacteriological studies, and isolates were obtained from the brain and kidneys. Bacterial identification using matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF/MS) revealed V. vulnificus. One isolate, RP4, was used for identification via dnaJ and 16S rRNA gene sequencing and antimicrobial susceptibility evaluation. Sorubim juveniles were experimentally challenged with RP4 isolate via intracelomic injection (IC, 107 colony-forming units [CFU] fish−1) and immersion bath (IB, 106 CFU mL−1 for 30 min). Identities to V. vulnificus of dnaJ and 16S rRNA genes by BLAST analysis were higher than 92% and 98%, respectively. Susceptibility to oxytetracycline, florfenicol, and other antimicrobial molecules was also observed. In the IC-challenged group, the mortality rate was 100% with V. vulnificus recovered from fish organs (brain, liver, spleen, and kidney). Additionally, splenic endothelium alterations were observed in the IC group. On the contrary, the control and IB groups did not develop any clinical signs, mortality, or bacterial recovery after 7 days of challenge. To the best of our knowledge, this is the first report of pathogenic V. vulnificus in farmed Pseudoplatystoma sp.

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Faecal microbiota dynamics in Cornu aspersum during dietary change and antibiotic challenge

Smith

Boomer

Baltzley

2019

Journal of Molluscan Studies

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Despite its widespread geographic distribution and applications, the herbivorous land snail Cornu aspersum represents an understudied animal host. In this study, we used a culture-based approach, in combination with a population analysis based on 16S rRNA amplicon sequencing, to describe changes in the faecal microbiota of wild-caught snails that were fed a nonsterile plant diet over a period of 21 days. At the beginning of the experiment, wild-caught snails harboured diverse populations of Gammaproteobacteria. The two most abundant genera of Gammaproteobacteria were soil-associated Pseudomonas and Buttiauxella; the latter is an understudied genus often reported in snails and slugs. During penicillin treatment, the diversity of Gammaproteobacteria in experimental snails bottlenecked, resulting in Pseudomonas takeover. Following penicillin treatment, experimental snails began to recover, with faecal samples containing pre-treatment Gammaproteobacteria groups by day 21. There was a significant difference in the colony-forming units/g across days and across treatments. Additionally, there was a significant interaction between day and treatment. The food source was not sterile and supported its own population of Gammaproteobacteria, primarily Pseudomonas, which would have been taken up by the snails daily. Given that Buttiauxella was not observed on the food source, we hypothesize that it was present and maintained within the digestive tract of C. aspersum, and this is consistent with the idea that C. aspersum maintains an endogenous gut microbiome.

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Enterobacteria and Vibrio from Macrobrachium amazonicum prawn farming in Fortaleza, Ceará, Brazil

Cited by 3 publications

References 20 publications

Engenharia de Alimentos: tópicos físicos, químicos e biológicos - Volume 1

Engenharia de Alimentos: tópicos físicos, químicos e biológicos - Volume 1

First Report of Vibrio vulnificus Outbreak in Farm-Raised Sorubim (Pseudoplatystoma sp.) from Brazil

Faecal microbiota dynamics in Cornu aspersum during dietary change and antibiotic challenge

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