Multiple shoots differentiated from hypocotyl explants of Sesbania aculeata (Pers.) syn S. cannabina (Retz.) Pers., a leguminous woody shrub, when cultured on Murashige and Skoog's basal medium supplemented with auxin (IBA, NAA) or auxin and cytokinin (IBA + BAP, NAA + BAP). Shoot budding occurred directly from the explant as well as from callus. Differentiation of shoot and root occurred in one step in the same concentration of auxin or auxin and cytokinin. Elongation of shoots occurred in the shoot induction medium.
Micropropagation of mahua, Madhuca latifolia Macb. (Sapotaceae) was achieved by culturing excised nodes on WPM supplemented with different plant growth regulators. A combination of IBA with BA or kinetin was found suitable for axillary shoot initiation within a week of culture. Excised microshoots treated with IBA produced roots. Best regenerative response was observed from explants collected in May.
The nuclear structures in different tissues of a number of dicotyledonous angiosperms show that, in certain species, nuclear repatternings due to the close association of different heterochromatic chromosomal regions occur in differentiating and differentiated cells. Chromocenter association plays a role in or is a marker of events that bring into play a large-scale control mechanism of the functional activity of the nucleus that is exploited in cell differentiation from its early stages. The somatic chromosome numbers of the genus Oroxylum indicum reported are 2n = 28, 2n = 30 and 2n = 38. The present work attempts to gives an account of the correct chromosome number in relation to chromocenters in mitotic and meiotic cells of Oroxylum indicum (L.) Vent.
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