Isolation and characterization of halotolerant bacilli from chickpea (Cicer arietinum L.) rhizosphere for plant growth promotion and biocontrol traits

Sharma, Anjney; Kashyap, Prem Lal; Srivastava, Alok Kumar; Bansal, Y. K.; Kaushik, Rajeev

doi:10.1007/s10658-018-1592-7

Cited by 42 publications

(32 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fungus (FS/ RS/SR) inoculated Petri plate without endophytic strain was maintained as a control. The inhibition diameter (mm) and the percentage of mycelial growth inhibition by each strain of endophytic bacteria were determined by using the following formula proposed by Sharma et al [33].…”

Section: In Vitro Screening For Antagonistic Activitymentioning

confidence: 99%

Plant growth promoting and antifungal activity in endophytic Bacillus strains from pearl millet (Pennisetum glaucum)

et al. 2019

Self Cite

View full text Add to dashboard Cite

Bacterial endophytes are well known inhabitants of living plant system and perform important assignments in maintaining plant growth and health. Currently, limited reports are available on the endophytes of pearl millet (Pennisetum glaucum) reflecting antagonistic and plant growth promoting (PGP) attributes. Therefore, the major objectives of current investigation were to identify antagonistic strains of endophytic Bacillus from pearl millet and further illustrate their PGP capabilities. In this study, 19 endophytic Bacillus strains (EPP5,

show abstract

Section: In Vitro Screening For Antagonistic Activitymentioning

confidence: 99%

Plant growth promoting and antifungal activity in endophytic Bacillus strains from pearl millet (Pennisetum glaucum)

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…Dual plate confrontation (Sharma et al 2018) and volatile metabolite assays (Fernando et al 2005) were conducted to confirm the antagonistic potential of test isolates against R. solani AG-4 on solidified PDA medium amended with 0, 50, 100,150, 200, and 250 mM NaCl. In order to perform the volatile metabolite assay (Fig.…”

Section: In Vitro Antagonism Assay and Hydrolytic Enzyme Estimationmentioning

confidence: 99%

“…The utilization of salt-tolerant antagonists emerged as a valuable microbial resource to protect agricultural crops against phytopathogenic fungi in saline terrestrial niches (Sharma et al 2018;Sadeghi et al 2017;Gal-Hemed et al 2011). Mohamed and Haggag (2006) reported that under saline conditions, salt-tolerant mutants of Trichoderma harzianum greatly surpassed their wild-type strain in growth rate, sporulation, and biological proficiency against Fusarium oxysporum responsible for causing tomato wilt.…”

Section: Introductionmentioning

confidence: 99%

Biocontrol Potential of Salt-Tolerant Trichoderma and Hypocrea Isolates for the Management of Tomato Root Rot Under Saline Environment

Kashyap

Solanki

Kushwaha

et al. 2019

J Soil Sci Plant Nutr

Self Cite

View full text Add to dashboard Cite

The aim of present study was to decipher the effect of salinity stress on growth and antagonistic potential of Trichoderma and Hypocrea isolates against tomato root rot pathogen (Rhizoctonia solani AG-4) under saline soil conditions. In vitro salinity assays, dual plate confrontation, and volatile metabolite assays were employed to establish the antagonistic potential of Trichoderma and Hypocrea isolates against R. solani AG-4 under varied salt gradients. Potential Trichoderma and Hypocrea isolates were evaluated against tomato root rot disease in greenhouse conditions under salt stress condition. Polymerase chain reaction (PCR) assay was performed to confirm the presence of endochitinase gene in salt-tolerant antagonists. Enzyme and biochemical assays were conducted to define the role of compatible solutes and defense-related enzymes in controlling tomato root rot under saline soil conditions. Trichoderma and Hypocrea isolates were capable to grow and sporulate up to 250 mM NaCl and also showed strong antagonism against R. solani. Enzymatic estimation of hydrolytic enzymes and amplification of endochitinase gene suggested that the test isolates are potent antagonistic agents. Under greenhouse evaluation, Trichoderma and Hypocrea fortified tomato plants showed significant reduction in tomato root rot disease in saline soils over untreated control. Significant increase in total phenol, polyphenol oxidase, peroxidase, β-1,3-glucanase, phenylalanine lyase, chitinase, proline, reducing sugar, and total soluble sugar displayed direct association with salt stress tolerance. Application of salt-tolerant Trichoderma and Hypocrea isolates emerged as a simple, safe, and cheap method for the biological management of tomato root rot under saline condition.

show abstract

“…This kind of bacterial behavior is an added advantage to fight with soil-borne fungal plant pathogens under adverse conditions [33,34]. Analysis of post-interaction studies through SEM revealed that B. subtilis strain RH5 well colonized surrounding the fungal mycelia and inhibition of spore germination, cause cytoplasmic leakage and bifurcation in newly generating hyphae of R. solani [11].…”

Section: Discussionmentioning

confidence: 99%

“…For molecular identification of strain RH5, 16S rRNA gene amplification and sequencing were carried out. The universal forward and reverse primer; 27 F (5′-AGAG TTTGATCCTGGCTCAG-3′) and 1541 R (5′-AAGGAGG TGATCCAGCCGCA-3′) were used to amplify a near-full length (∼1,500 bp) fragment of 16S rRNA gene [11]. Polymerase chain reaction (PCR) reaction was carried out in a 100 µl reaction mixture containing 50 ng of bacterial DNA, reaction buffer (×10), 2.5 mM of each dNTP, 10 pM of each primer, and 1.0 U of Taq DNA polymerase (Banglore GeNei, India).…”

Section: Molecular Identificationmentioning

confidence: 99%

Biocontrol potential of Bacillus subtilis RH5 against sheath blight of rice caused by Rhizoctonia solani

et al. 2019

Self Cite

View full text Add to dashboard Cite

The sheath blight disease of rice caused by Rhizoctonia solani is widely prevalent and one of the most destructive diseases, affecting rice cultivation and loss worldwide. In the present study, a set of twenty Bacillus isolates from saline soil of Uttar Pradesh were tested for their biocontrol activity against R. solani with the aim to obtain a potential strain for the control of sheath blight disease toward ecofriendly and sustainable agriculture. The results of dual‐culture assay and scanning electron microscopic studies showed that the strain RH5 exhibited significant antagonistic activity (84.41%) against the fungal pathogen R. solani. On the basis of 16S rDNA sequencing analysis, the potential biocontrol strain RH5 was identified as Bacillus subtilis. Furthermore, the strain RH5 was characterized by different plant growth‐promoting (PGP) activities and induction of defense‐related enzymes in rice plants against R. solani. The strain RH5 posses various PGP attributes (indole acetic acid, siderophore, hydrogen cyanide production and phosphate, Zn, K solubility), hydrolytic enzymatic (chitinase, protease, cellulase, xylanase) activity, and presence of antimicrobial peptide biosynthetic genes (bacylisin, surfactin, and fengycin), which support the strain for efficient colonization of hyphae and its inhibition. Finally, the results of the greenhouse study confirmed that strain RH5 significantly increased plant growth and triggered resistance in rice plants through the production of defense‐related antioxidant enzymes.

show abstract

Isolation and characterization of halotolerant bacilli from chickpea (Cicer arietinum L.) rhizosphere for plant growth promotion and biocontrol traits

Cited by 42 publications

References 52 publications

Plant growth promoting and antifungal activity in endophytic Bacillus strains from pearl millet (Pennisetum glaucum)

Plant growth promoting and antifungal activity in endophytic Bacillus strains from pearl millet (Pennisetum glaucum)

Biocontrol Potential of Salt-Tolerant Trichoderma and Hypocrea Isolates for the Management of Tomato Root Rot Under Saline Environment

Biocontrol potential of Bacillus subtilis RH5 against sheath blight of rice caused by Rhizoctonia solani

Contact Info

Product

Resources

About