Changes in enzyme activities, soluble sugars and phenols during in vitro adventitious shoot regeneration from leaflet explants of Albizia procera (Roxb.) Benth

Sarkar, Anindya; Rai, Ekta; Shah, Syed Naseer; Bouddha, Sulochna; Bansal, Y. K.; Ansari, Shamim Akhtar

doi:10.1007/s11056-010-9202-3

Cited by 5 publications

(3 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The SS and OS content showed a positive correlation with the progression of root organogenesis and a negative correlation with the progression of shoot organogenesis. Many papers have reported an initial accumulation, followed by a decline of saccharides during in vitro organogenesis (Chatrath et al 1996, Vaz et al 1998, Wu et al 1999, Kromer and Gamian 2000, Huang and Liu 2002, Panigrahi et al 2007, Sarkar et al 2010. The SS, RS, and OS content decreased during saffron shoot organogenesis and the sugars are important sources of energy and carbon skeletons (Sarkar et al 2010) during shoot primordia differentiation and organization.…”

Section: Resultsmentioning

confidence: 99%

“…Many papers have reported an initial accumulation, followed by a decline of saccharides during in vitro organogenesis (Chatrath et al 1996, Vaz et al 1998, Wu et al 1999, Kromer and Gamian 2000, Huang and Liu 2002, Panigrahi et al 2007, Sarkar et al 2010. The SS, RS, and OS content decreased during saffron shoot organogenesis and the sugars are important sources of energy and carbon skeletons (Sarkar et al 2010) during shoot primordia differentiation and organization. The PS accumulation in a nodular callus undergoing shoot regeneration is a prerequisite for in vitro shoot development (Medina et al 1998).…”

Section: Resultsmentioning

confidence: 99%

“…Through their interaction with growth regulators, phenolics intervene in the regulation of developmental processes such as organogenesis (Kanmegne and Omokolo 2003). Adventitious shoot regeneration from leaflet explants of Albizia procera have been related to the changes in phenolic content (Sarkar et al 2010). It seems that the increased content of phenolics during formation of nodular callus is associated with their growth regulating properties, and decreased content during organ formation is associated with lignification during cellular differentiation and organization into shoot primordia.…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Histological and biochemical parameters of Crocus sativus during in vitro root and shoot organogenesis

Vatankhah¹,

Niknam²,

Ebrahimzadeh³

2014

Biol. plant.

View full text Add to dashboard Cite

Content of malondialdehyde (MDA), proline, phenolics, and saccharides was analyzed during different developmental stages of in vitro root and shoot organogenesis in saffron. The highest content of MDA, proline, and phenolics was detected in nodular calli. Significant changes were also found in the content of polysaccharides, soluble saccharides, oligosaccharides, and reducing saccharides during developmental stages. Histological investigation of nodular calli showed meristematic zones with small and densely stained cells situated at peripheral zones of calli. The meristematic zones surrounded some vascular areas from which de novo organs originated. The parenchymatic cells of inner zones of calli converted to procambium cells that produced vascular tissues.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Histological and biochemical parameters of Crocus sativus during in vitro root and shoot organogenesis

Vatankhah¹,

Niknam²,

Ebrahimzadeh³

2014

Biol. plant.

View full text Add to dashboard Cite

show abstract

Biochemical changes in embryogenic and non-embryogenic callus of Bambusa nutans Wall. during somatic embryogenesis

Rajput

Rathore

Ansari

et al. 2023

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Bambusa nutans Wall. is a clump-forming, evergreen bamboo species that is most often found in Southeast Asian forests. Comparative activities of nitrate reductase (NR), glutamine synthetase (GS) and peroxidase (POX) as well as expression of peroxidase isozymes during somatic embryogenesis (SE) were investigated in the segregated embryogenic callus (EC) and non-embryogenic callus (NEC) from the same genotype (CPC-648) of B. nutans Wall. The EC was compact, with large prominent nuclei and dense cytoplasm, while the NEC was fragile, with rudimentary nuclei and hyaline cytoplasm. SE in EC encompassed induction, maturation and germination stages each of 30 days on MS medium supplemented with ascorbic acid 50mg/l, citric acid 25mg/l, cysteine 25mg/l and glutamine 100mg/l + 2, 4-D-2 mg/l + coconut water 10%. EC exhibited dedifferentiation and growth leading to SE, while NEC remained amorphous loose lump throughout. EC had lower NR and POX activities and less number of peroxidase isozymes but higher GS activity than NEC during three stages of SE. NR activity followed a trend as induction > germination > maturation (EC and NEC); GS activity, maturation > germination >induction (EC) and induction >germination >maturation (NEC) and POX activity, maturation > induction > germination (EC) and induction > germination > maturation (NEC). Compared to NEC, EC exhibited less peroxidase isozymes. SE is an epigenetically regulated process that leads to the expression of enzymes involved in primary metabolism in EC and secondary metabolism in NEC. GS activity corresponding to SE process may be developed as biochemical marker. A chaotic scenario, rather from being a marker of SE, is indicated by high levels of peroxidase activity and isozyme expression.

show abstract

Evaluation of biochemical changes during different stages of somatic embryogenesis in a vulnerable timber tree Dalbergia latifolia (Indian rosewood)

Shirin

Bhadrawale

Mishra

et al. 2020

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

Changes in enzyme activities, soluble sugars and phenols during in vitro adventitious shoot regeneration from leaflet explants of Albizia procera (Roxb.) Benth

Cited by 5 publications

References 30 publications

Histological and biochemical parameters of Crocus sativus during in vitro root and shoot organogenesis

Histological and biochemical parameters of Crocus sativus during in vitro root and shoot organogenesis

Biochemical changes in embryogenic and non-embryogenic callus of Bambusa nutans Wall. during somatic embryogenesis

Evaluation of biochemical changes during different stages of somatic embryogenesis in a vulnerable timber tree Dalbergia latifolia (Indian rosewood)

Contact Info

Product

Resources

About