Synthetic Seeds: A Novel Approach of In Vitro Plantlet Formation in Vasaka (Adhatoda vasica Nees.).

Anand, Yogeeta; Bansal, Y. K.

doi:10.5511/plantbiotechnology.19.159

Cited by 34 publications

(19 citation statements)

References 3 publications

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“…1f). The conservation of germplasm through encapsulated method has been practiced relating to numerous medicinal plant species, such as Ocimum species (Mandal et al 2000), Adhatoda vasica (Anand and Bansal 2002), Withnia somnifera (Singh et al 2006b), Rauvolfia serpentina (Ray and Bhattacharya 2008), Cannabis sativa (Lata et al 2009), etc. but the development of whole plant through encapsulated microshoots has been not reported in P. kurrooa.…”

Section: Resultsmentioning

confidence: 98%

“…Although synthetic seeds have been widely utilized for micropropagation and conservation of various medicinal plant species Anand and Bansal 2002;Nyende et al 2003;Manjkhola et al 2005;Singh et al 2006a, b;Narula et al 2007;Ray and Bhattacharyaa 2008;Lata et al 2009), the genetic stability of synthetic seed-derived plantlets remains relatively unknown with the exception of a recent report on Ananus comosus (Gangopadhyay et al 2005). In contrast, the impact of cryogenic conservation, via encapsulation/ dehydration or verification methods, of plant germplasm on observed genetic variability has attracted more attention (Chandel and Chaudhury 1995;Ashmore 1997).…”

Section: Introductionmentioning

confidence: 97%

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Assessment of genetic fidelity of encapsulated microshoots of Picrorhiza kurrooa

Mishra

Singh

Palni

et al. 2010

Plant Cell Tiss Organ Cult

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In vitro grown microshoots of Picrrhiza kurrooa were encapsulated in the alginate beads. Regrowth of encapsulated microshoots, using alginate encapsulation, of P. kurrooa reached 89.33% following 3 months of storage. Amongst developing plantlets, 42.66% exhibited formation of multiple shoots at the onset of regrowth and 21.43% demonstrated simultaneous formation of shoots and roots. Healthy root formation was observed in plantlets following 2 weeks of their transfer to half-strength Murashige and Skoog medium containing 1 lM a-naphthalene acetic acid. Plants were transplanted to the greenhouse in three batches with 95% frequency of survival. The genetic fidelity of P. kurrooa plants growing out after storage in encapsulated form was ascertained by random amplified polymorphic DNA (RAPD) analysis. Molecular analysis of randomly selected plants from each batch was conducted using 45 random decamer primers. Of 45 primes tested, 14 produced scorable amplified products. Total 68 bands were observed amongst them 7.35% bands were polymorphic. Cluster analysis of the RAPD profile revealed an average similarity coefficient of 0.966 thus confirming genetic stability of plants derived from encapsulated microshoots following 3 months of storage.

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Section: Resultsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 97%

Assessment of genetic fidelity of encapsulated microshoots of Picrorhiza kurrooa

Mishra

Singh

Palni

et al. 2010

Plant Cell Tiss Organ Cult

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“…1f). It is pertinent to mention here that, conservation of germplasm through encapsulation method has been practiced relating to numerous medicinal plant species, such as Ocimum species (Mandal et al 2000), Adhatoda vasica (Anand and Bansal 2002), Phyllanthus amarus (Singh et al2006a), Withnia somnifera (Singh et al 2006b), Dioscorea bulbifera (Narula et al 2007), Tylophora indica (Faisal and Anis 2007), Rauvolfia serpentina (Ray and Bhattacharyaa 2008), Cannabis sativa (Lata et al 2009), etc., but unlike the present study, regrowth of encapsulated explant into complete plantlets has, by and large, been analyzed on PGR supplemented media. Moreover, this study reports successful storage of the encapsulated microshoots of C. maritima at 25 ± 2°C in contrast to several of the above cited reports where storage has been carried out at 4°C (Mandal et al 2000;Singh et al2006a, b;Narula et al 2007;Faisal and Anis 2007).…”

Section: Resultsmentioning

confidence: 99%

“…Although synthetic seeds have been widely utilized for micro propagation and conservation of various medicinal plant species (Ara et al 2000;Mandal et al 2000;Anand and Bansal 2002;Nyende et al 2003;Manjkhola et al2005;Singh et al2006a, b;Narula et al 2007;Faisal and Anis 2007;Ray and Bhattacharyaa 2008;Lata et al 2009), the genetic stability of synthetic seed-derived plantlets remains relatively unknown with the exception of a recent report on Ananus comosus (L.) Merr (Gangopadhyay et al 2005). In contrast, the impact of cryogenic conservation, via encapsulation/ dehydration or vitrification methods, of plant germplasm on observed genetic variability has attracted more attention (Chaudhary et al 1989;Chandel and Chaudhary 1995;Ashmore 1997).…”

Section: Introductionmentioning

confidence: 97%

An evaluation of genetic fidelity of encapsulated microshoots of the medicinal plant: Cineraria maritima following six months of storage

Srivastava

Khan

Banerjee

2009

Plant Cell Tiss Organ Cult

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Regrowth of encapsulated microshoots, using alginate encapsulation, of Cineraria maritima reached 82.35% following 6 months of storage. Amongst developing plantlets, 33.33% exhibited formation of multiple shoots at the onset of regrowth and 11.76% demonstrated simultaneous formation of shoots and roots. Healthy root formation was observed in plantlets following 2 weeks of their transfer to half-strength Murashige and Skoog medium containing 1.0 mg l -1 a-naphthalene acetic acid. Plants were transplanted to the greenhouse in three batches with 90% frequency of survival. Molecular analysis of randomly selected plants from each batch was conducted using 20 random amplified polymorphic DNA (RAPD) markers. Of 20 primers tested, 14 produced amplification products, and a total of 69 bands with an average of 4.93 bands per primer were observed. Of these 69 scorable bands, only 20% of bands were polymorphic. Cluster analysis of the RAPD profiles revealed an average similarity coefficient of 0.944 thus confirming molecular stability of plants derived from encapsulated microshoots following 6 months of storage.

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“…Thus, it is important to assess the genetic stability of the conserved propagules. Although many reports are available on the utilization of synthetic seeds for micro propagation and conservation of various medicinal plant species (Mandal et al 2000;Anand and Bansal 2002;Singh et al 2006;Narula et al 2007;Faisal and Anis 2007;Ray and Bhattacharyaa 2008;Lata et al 2009b), there are very few studies on genetic stability of synthetic seed-derived plantlets exist (Gangopadhyay et al 2005;Srivastava et al 2009;Mishra et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Molecular analysis of genetic fidelity in Cannabis sativa L. plants grown from synthetic (encapsulated) seeds following in vitro storage

et al. 2011

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The increasing utilization of synthetic (encapsulated) seeds for germplasm conservation and propagation necessitates the assessment of genetic stability of conserved propagules following their plantlet conversion. We have assessed the genetic stability of synthetic seeds of Cannabis sativa L. during in vitro multiplication and storage for 6 months at different growth conditions using inter simple sequence repeat (ISSR) DNA fingerprinting. Molecular analysis of randomly selected plants from each batch was conducted using 14 ISSR markers. Of the 14 primers tested, nine produced 40 distinct and reproducible bands. All the ISSR profiles from in vitro stored plants were monomorphic and comparable to the mother plant which confirms the genetic stability among the clones. GC analysis of six major cannabinoids [Δ(9)-tetrahydrocannabinol, tetrahydrocannabivarin, cannabidiol, cannabichromene, cannabigerol and cannabinol] showed homogeneity in the re-grown clones and the mother plant with insignificant differences in cannabinoids content, thereby confirming the stability of plants derived from synthetic seeds following 6 months storage.

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Synthetic Seeds: A Novel Approach of In Vitro Plantlet Formation in Vasaka (Adhatoda vasica Nees.).

Cited by 34 publications

References 3 publications

Assessment of genetic fidelity of encapsulated microshoots of Picrorhiza kurrooa

Assessment of genetic fidelity of encapsulated microshoots of Picrorhiza kurrooa

An evaluation of genetic fidelity of encapsulated microshoots of the medicinal plant: Cineraria maritima following six months of storage

Molecular analysis of genetic fidelity in Cannabis sativa L. plants grown from synthetic (encapsulated) seeds following in vitro storage

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