An evaluation of genetic fidelity of encapsulated microshoots of the medicinal plant: Cineraria maritima following six months of storage

Abstract

Regrowth of encapsulated microshoots, using alginate encapsulation, of Cineraria maritima reached 82.35% following 6 months of storage. Amongst developing plantlets, 33.33% exhibited formation of multiple shoots at the onset of regrowth and 11.76% demonstrated simultaneous formation of shoots and roots. Healthy root formation was observed in plantlets following 2 weeks of their transfer to half-strength Murashige and Skoog medium containing 1.0 mg l -1 a-naphthalene acetic acid. Plants were transplanted to the greenhouse in three batches with 90% frequency of survival. Molecular analysis of randomly selected plants from each batch was conducted using 20 random amplified polymorphic DNA (RAPD) markers. Of 20 primers tested, 14 produced amplification products, and a total of 69 bands with an average of 4.93 bands per primer were observed. Of these 69 scorable bands, only 20% of bands were polymorphic. Cluster analysis of the RAPD profiles revealed an average similarity coefficient of 0.944 thus confirming molecular stability of plants derived from encapsulated microshoots following 6 months of storage.

Molecular analysis of genetic fidelity in Cannabis sativa L. plants grown from synthetic (encapsulated) seeds following in vitro storage

“…Our results corroborate with reports of genetic stability of synthetic seed derived plants of Ananus comosus (Gangopadhyay et al 2005), Cineraria maritime (Srivastava et al 2009) and Picrorhiza kurrooa (Mishra et al 2011) after short term storage period. Furthermore, our results using GC analysis also showed homogeneity in the cannabinoids profile and cannabinoids content of the mother plant and the randomly selected clones propagated through synthetic seeds following storage under slow growth conditions (5, 15 and 25°C) for 6 months (Fig.…”

“…Thus, it is important to assess the genetic stability of the conserved propagules. Although many reports are available on the utilization of synthetic seeds for micro propagation and conservation of various medicinal plant species (Mandal et al 2000;Anand and Bansal 2002;Singh et al 2006;Narula et al 2007;Faisal and Anis 2007;Ray and Bhattacharyaa 2008;Lata et al 2009b), there are very few studies on genetic stability of synthetic seed-derived plantlets exist (Gangopadhyay et al 2005;Srivastava et al 2009;Mishra et al 2011).…”

Efficient long-term conservation of Taraxacum pieninicum synthetic seeds in slow growth conditions

“…This research is consistent with reports of genetic stability (RAPD) of stored synthetic seed derived plants of Ananus comosus after 2 months at 8 °C (Gangopadhyay et al 2005), Cineraria maritime after 6 months at 25 ± 2 °C (Srivastava et al 2009), Cannabis sativa after 6 months at 5, 15 and 25 °C (Lata et al 2011), Picrorhiza kurrooa after 3 months at 25 ± 2 °C (Mishra et al 2011), Glycyrrhiza glabra after 6 months at 25 ± 2 °C (Mehrotra et al 2012) and Ocimum kilimandscharicum after 3 months at 4 and 25 °C (Saha et al 2015). Hao and Deng (2003) noted that AFLP assay found no genetic variations between non-stored and stored at 4 °C for 1 year shoot tips of Malus pumila cv.…”

A Protocol For Synthetic Seeds From Salvia Officinalis L. Shoot Tips

“…A synthetic seed is a somatic embryo or any non-embryogenic vegetative propagule (shoot tip, shoot primordial, axillary bud, nodal segment) inside an alginate hydrogel coating, possessing the ability to convert to a plant in vitro or ex vitro, an ability it can retain after storage. The use of unipolar propagules for development of synthetic seeds has been reported in numerous medical plant species, such as Valeriana wallichii (Mathur et al, 1989), Rauvolfia serpentina (Ray and Bhattacharya, 2008), Cineraria maritima (Srivastava et al, 2009), Cannabis sativa (Lata et al, 2009), Fragaria ananasa and Rubus idaeus (Lisek and Orlikowska, 2004). This kind of explant is very useful in such plant species, where somatic embryogenesis is not well established or else good quality somatic embryos are not produced.…”