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Consider a system of N parallel single-server queues with unit-exponential service time distribution and a single dispatcher where tasks arrive as a Poisson process of rate λ(N). When a task arrives, the dispatcher assigns it to one of the servers according to the Join-the-Shortest Queue (JSQ) policy. Eschenfeldt and Gamarnik (2015) established that in the Halfin-Whitt regime where (N − λ(N))/ √ N → β > 0 as N → ∞, appropriately scaled occupancy measure of the system under the JSQ policy converges weakly on any finite time interval to a certain diffusion process as N → ∞. Recently, it was further established by Braverman (2018) that the convergence result extends to the steady state as well, i.e., stationary occupancy measure of the system converges weakly to the steady state of the diffusion process as N → ∞, proving the interchange of limits result.In this paper we perform a detailed analysis of the steady state of the above diffusion process. Specifically, we establish precise tail-asymptotics of the stationary distribution and scaling of extrema of the process on large time interval. Our results imply that the asymptotic steady-state scaled number of servers with queue length two or larger exhibits an Exponential tail, whereas that for the number of idle servers turns out to be Gaussian. From the methodological point of view, the diffusion process under consideration goes beyond the state-of-the-art techniques in the study of the steady state of diffusion processes. Lack of any closed form expression for the steady state and intricate interdependency of the process dynamics on its local times make the analysis significantly challenging. We develop a technique involving the theory of regenerative processes that provides a tractable form for the stationary measure, and in conjunction with several sharp hitting time estimates, acts as a key vehicle in establishing the results. The technique and the intermediate results might be of independent interest, and can possibly be used in understanding the bulk behavior of the process.

A protocol for induction and establishment of Agrobacterium rhizogenes-mediated hairy root cultures of Picrorhiza kurroa was developed through optimization of the explant type and the most suitable bacterial strain. The infection of leaf explants with the LBA9402 strain resulted in the emergence of hairy roots at 66.7% relative transformation frequency. Nine independent, opine and TL-positive hairy root clones were studied for their growth and specific glycoside (i.e., kutkoside and picroside I) productivities at different growth phases. Biosynthetic potentials for the commercially desirable active constituents have been expressed by all the tested hairy root clones, although distinct inter-clonal variations could be noted in terms of their quantity. The yield potentials of the 14-P clone, both in terms of biomass as well as individual glycoside contents (i.e., kutkoside and picroside I), superseded that of all other hairy root clones along with the non-transformed, in vitro-grown control roots of P. kurroa. The present communication reports the first successful establishment, maintenance, growth and selection of superior hairy root clone of Picrorhiza kurroa with desired phyto-molecule production potential, which can serve as an effective substitute to its roots and thereby prevent the indiscriminate up-rooting and exploitation of this commercially important, endangered medicinal plant species.

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Tissue specific biosynthesis of secondary metabolites is a distinguished feature of medicinal plants. Withania somnifera, source of pharmaceutically important withanolides biosynthesizes withaferin-A in leaves and withanolide-A in roots. To increase the in planta withanolides production, a sustainable approach needs to be explored. Here, we isolated endophytes from different parts of W. somnifera plants and their promising role in in planta withanolide biosynthesis was established in both in-vivo grown as well in in-vitro raised composite W. somnifera plants. Overall, the fungal endophytes improved photosynthesis, plant growth and biomass, and the root-associated bacterial endophytes enhanced the withanolide content in both in-vivo and in-vitro grown plants by modulating the expression of withanolide biosynthesis genes in leaves and roots. Surprisingly, a few indole-3-acetic acid (IAA)-producing and nitrogen-fixing root-associated endophytes could induce the biosynthesis of withaferin-A in roots by inducing in planta IAA-production and upregulating the expression of withanolide biosynthesis genes especially MEP-pathway genes (DXS and DXR) in roots as well. Results indicate the role of endophytes in modulating the synthesis and site of withanolides production and the selected endophytes can be used for enhancing the in planta withanolide production and enriching roots with pharmaceutically important withaferin-A which is generally absent in roots.

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