A Protocol For Synthetic Seeds From Salvia Officinalis L. Shoot Tips

Grzegorczyk, Izabela; Wysokińska, Halina

doi:10.2478/v10182-011-0011-6

Cited by 15 publications

(11 citation statements)

References 19 publications

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“…This explains increasing the regrowth percentage of propagules coated with MS containing matrixes. The inclusion of sucrose in the alginate matrix also improved the regrowth percentage from encapsulated shoot tips of Camelia japonica and axillary buds of Betula pendula (Grzegorczyk and Wysokinska, 2011). Rai et al (2008) proved that; the emergence of shoots from encapsulated shoot tips of Psidium guajava was completely inhibited in medium lacking sucrose.…”

Section: Table (1) Effect Of Storage Temperature and Alginate Matrixmentioning

confidence: 84%

“…This demonstrates that, MS medium plays a vital role and considers an important ingredient in Na-alginate matrix for regrowth. Piccioni and Standardi (1995) and Grzegorczyk and Wysokinska (2011) reported that; the addition of MS to the alginate matrix led to reduction in the gel viscosity. This explains increasing the regrowth percentage of propagules coated with MS containing matrixes.…”

Section: Table (1) Effect Of Storage Temperature and Alginate Matrixmentioning

confidence: 99%

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In Vitro Preservation of the ‎egyptian Endemic Silene ‎schimperiana Boiss. Plant via ‎encapsulation

Ghareb

2021

Egyptian Journal of Desert Research

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ilene schimperiana (Caryophyllaceae) is an endemic plant of Saint Katherine Protectorate, Sinai, Egypt. This genus has various medicinal properties. Due to over exploitation, S. schimperiana is at risk of extinction at the vulnerable stage in Egypt. Therefore, its conservation is important. An efficient protocol for S. schimperiana synthetic seeds production was developed in the present study. Shoot tips and nodal segments obtained from in vitro proliferated shoots were encapsulated in calcium alginate beads for medium term conservation and germplasm exchange. Encapsulated propagules were stored for five months at 4°C in three different matrixes of calcium alginate; water, Murashige and Skoog (MS) medium and MS medium containing 3% sucrose. The most efficient gel complex for formation of identical beads was 3% Na-alginate and 100 mM CaCl2.2H2O. The optimum temperature for storage was 4°C with 100% viability during the period of five months of storage with all tested alginate matrix compositions. However, the matrix containing MS medium with 3% sucrose was the best for the encapsulated propagules. The regrowth frequency of encapsulated propagules and mean number of shoots were decreased by increasing in storage duration. Rooting was achieved on ¼ MS medium supplemented with 1.0 mg L-1 indole-3-butyric acid (IBA) and 2 mg L-1 α-naphthalene acetic acid (NAA). About 90% of regenerated plantlets were successfully acclimatized under greenhouse conditions. This method can be successfully applied for in vitro preservation of S. schimperiana propagules for five months at 4°C to conserve this valuable plant.

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Section: Table (1) Effect Of Storage Temperature and Alginate Matrixmentioning

confidence: 84%

Section: Table (1) Effect Of Storage Temperature and Alginate Matrixmentioning

confidence: 99%

In Vitro Preservation of the ‎egyptian Endemic Silene ‎schimperiana Boiss. Plant via ‎encapsulation

Ghareb

2021

Egyptian Journal of Desert Research

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“…Even it was reported that the high concentration or the long exposure of the embryos to the calcium chloride results in more absorption and penetration of CaCl2 in the embryo, which can lead to growth inhibition [21]. But based on Fig.2, it can be seen that the percentage of germination of synthetic chrysanthemum seeds showed that there were not significantly different at each storage period.…”

Section: A Encapsulationmentioning

confidence: 87%

Encapsulation and Germination of Synthetic Seeds of Chrysanthemum

Nugrahani¹,

Moeljani²,

Lydiana³

2018

Proceedings of the International Conference on Science and Technology (ICST 2018)

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Chrysanthemum is one of the most important Indonesia flowers, that which has always been developed in various plant breeding studies, including the development of synthetic seed encapsulation methods.Encapsulation is a technology on enclosed any meristematic plant tissue in a protective and nutritive matrix under in vitro or in vivo environmental conditions, also after transport and/or storage. The study were aimed at developing a method of encapsulating shoot tip and nodal segments of Chrysanthemum that can be used for plant propagation and as explants for short-term storage. Shoot tip and nodal segments of Chrysanthemum were used as explants for synthetic seed. The Murashige & Skoog (MS) basal medium was prepared with 30 g sucrose and 4% of sodium alginate added with growth regulator (IBA) and Coconut Water (CW). The explants droplets, each containing one shoot tip, were then maintained in 2% CaCl2 solution for polymerization. The seeds that have been formed were rinsed with distilled water sterile 3 times and dried at room temperature. The synthetic seeds were germinated on MS basal medium, without any plant growth regulators. Encapsulated seeds had a higher germination percentage in MS salt solution as the gel matrix supplemented with 150 ml/L coconut water. The viability of synthetic seeds was decrease on 4 weeks storage period, compared to the 1, 2 and 3 week's storage period.

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“…This suggests a significant impact of genetic factors on this trait. Perhaps the addition of auxins or gibberellic acid (GA 3 ) to the artificial endosperm could improve rooting in bleeding heart, as reported with synthetic seeds of Salvia officinalis L. [32].…”

Section: Morphogenetic Response Of Explantsmentioning

confidence: 97%

Shoot Tip Cryopreservation of Lamprocapnos spectabilis (L.) Fukuhara Using Different Approaches and Evaluation of Stability on the Molecular, Biochemical, and Plant Architecture Levels

Kulus

2020

IJMS

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The aim of this study is to optimize and evaluate the effectiveness of vitrification, droplet-vitrification, and encapsulation-vitrification techniques in the cryopreservation of Lamprocapnos spectabilis (L.) Fukuhara ‘Gold Heart’, a popular medicinal and ornamental plant species. In vitro-derived shoot tips were used in the experiments. All three techniques were based on explant dehydration with plant vitrification solution 3 (PVS3; 50% glycerol and 50% sucrose) for 0, 30, 60, 90, 120, 150, or 180 min. The recovered microshoots were subjected to morphometric, biochemical, and molecular analyses (RAPD, ISSR, SCoT). The highest recovery level was reported with the encapsulation-vitrification protocol based on 150 min dehydration (73.1%), while the vitrification technique was the least effective (maximum 25.8% recovery). Explants cryopreserved with the encapsulation-vitrification technique produced the highest mean number of shoots (4.9); moreover, this technique was optimal in terms of rooting efficiency. The highest fresh weight of shoots, on the other hand, was found with the vitrification protocol based on a 30-min PVS3 treatment. The concentrations of chlorophyll a and b were lower in all cryopreservation-derived plants, compared to the untreated control. On the other hand, short dehydration and cryopreservation of non-encapsulated explants stimulated the synthesis of anthocyanins. A small genetic variation in 5% of all samples analyzed was detected by RAPD and ISSR marker systems. Only plants recovered from the encapsulation-vitrification protocol had no DNA sequence alternations.

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A Protocol For Synthetic Seeds From Salvia Officinalis L. Shoot Tips

Cited by 15 publications

References 19 publications

In Vitro Preservation of the ‎egyptian Endemic Silene ‎schimperiana Boiss. Plant via ‎encapsulation

In Vitro Preservation of the ‎egyptian Endemic Silene ‎schimperiana Boiss. Plant via ‎encapsulation

Encapsulation and Germination of Synthetic Seeds of Chrysanthemum

Shoot Tip Cryopreservation of Lamprocapnos spectabilis (L.) Fukuhara Using Different Approaches and Evaluation of Stability on the Molecular, Biochemical, and Plant Architecture Levels

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