In vitro regeneration of Guizotia abyssinica Cass. and evaluation of genetic fidelity through RAPD markers

Baghel, Sapna; Bansal, Y. K.

doi:10.1016/j.sajb.2017.01.002

Cited by 10 publications

(8 citation statements)

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“…Using different PGRs is a major contributing factor to the change in plant genetics. The growth conditions such as the use of different light spectra or even the number of times the culture is subculture also affect the plant genetics (Huang et al 2009; Butiuc-Keul et al 2016; Baghel and Bansal, 2017). Somaclonal variations in plants can also occur due to the difference in the in vitro growth conditions such as different light spectra and the photoperiod.…”

Section: Introductionmentioning

confidence: 99%

“…PCR based single primer ampli cation reaction (SPAR) techniques using microsatellites, simple sequence repeats (SSR), inter simple sequence repeats (ISSR), random ampli ed polymorphic DNA (RAPD), and directed ampli cation of minisatellite DNA (DAMD) primers to analyse and detect the genetic stability among the in vitro generated plants are the most common practices in plant tissue culture experiments. Techniques which utilise single primers to amplify the DNA in PCR reactions are generally applicable universally (Zoghlami et al 2012;Lamare and Rao, 2015;Baghel and Bansal, 2017;Rohela et al 2019). The use of ISSR and DAMD markers has proven to be unbiased and cost-effective as compared with the RAPD markers (Singh et al 2014;Purayil et al 2018;Meena et al 2019).…”

Section: Introductionmentioning

confidence: 99%

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Assessment of genetic stability on in vitro and ex vitro plants of Ficus carica var. black jack using ISSR and DAMD markers

2021

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In vitro propagation has been signi cant in producing a large number of genetically stable regenerated plants. Regenerated Ficus carica var. Black Jack plantlets were established using woody plant medium (WPM) supplemented with 20 µM 6-Benzylaminopurine (BAP) and 8 µM Indole-3-acetic acid (IAA) under different light treatments such as normal uorescent white light (60 µmol.m − 2 .s − 1 ), and four different LED spectra, white (400-700nm), blue (440nm), red (660nm) and blue + red (440nm + 660nm). Genetic stability analysis was performed on the in vitro and ex vitro plants of Ficus carica var. Black Jack. Ten (10) primers of each ISSR and DAMD molecular marker were used to assess the genetic stability of the eight (8) samples of Ficus carica var. Black Jack, acquired over two years. The ndings of this study revealed that inter simple sequence repeats (ISSR) and directed ampli cation of minisatellite DNA (DAMD) markers (DNA primers) are e cient in determining the polymorphism and monomorphism percentage among the in vitro and ex vitro samples of Ficus carica var. Black Jack. ISSR markers showed 97.87% of monomorphism whereas DAMD markers showed 100% monomorphism. Polymorphism of 2.13% was observed for the UBC840 ISSR -DNA primer which was negated under the genetic similarity index analysis for the eight samples. It is recommended that genetic stability analysis should be performed for long-term maintenance of micropropagated plants.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Assessment of genetic stability on in vitro and ex vitro plants of Ficus carica var. black jack using ISSR and DAMD markers

2021

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“…RAPD fragments are easily separated in agarose gels by electrophoresis. The gel is stained with ethidium bromide or any other appropriate stain and visualized by ultraviolet (UV) illumination ( Baghel and Bansal, 2017 ). Complex patterns of PCR products are generated by the random annealing of the arbitrary RAPD primers to various locations in the genome of the target organism ( Figure 4 C).…”

Section: The Concept Of Molecular or Dna Markersmentioning

confidence: 99%

“…The variation in the banding patterns or the polymorphism shown for each accession ( Figure 4 C), arises from the presence and/or absence of different types of mutations that determine the binding or non-binding of the respective primers to different sections of the DNA of each of the accessions. RAPD polymorphisms are detected as present or absent of DNA bands mainly due to sequence size variations between primer binding sites with the target DNA of individuals ( Baghel and Bansal, 2017 ). In principle, RAPD polymorphisms arise primarily from variation in the primer hybridization or annealing positions in the target genome.…”

Section: The Concept Of Molecular or Dna Markersmentioning

confidence: 99%

Basic concepts and methodologies of DNA marker systems in plant molecular breeding

Amiteye

2021

Heliyon

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The concepts, methodologies and applications of some of the major molecular or DNA markers commonly used in plant science have been presented. The general principles of molecular marker techniques have been elucidated with detailed explanation of some notable basic concepts associated with marker applications: marker polymorphism, dominant or co-dominant mode of inheritance, agronomic trait-marker linkage, genetic mutations and variation. The molecular marker methods that have been extensively reviewed are RFLP, RAPD, SCAR, AFLP, SSR, CpSSR, ISSR, RAMP, SAMPL, SRAP, SSCP, CAPS, SNP, DArT, EST, and STS. In addition, the practicality of the retrotransposon-based marker methods, IRAP, REMAP, RBIP, and IPBS, have been discussed. Moreover, some salient characteristics of DNA markers have been compared and the various marker systems classified as PCR-or non-PCR-based, dominantly or co-dominantly inherited, locus specific or non-specific as well as at the levels of marker polymorphism and efficiency of marker reproducibility. Furthermore, the principles and methods of the following DNA markers have been highlighted: Penta-primer amplification refractory mutation system (PARMS), Conserved DNA-Derived Polymorphism (CDDP), P450-based analogue (PBA) markers, Tubulin-Based Polymorphism (TBP), Inter-SINE amplified polymorphism (ISAP), Sequence specific amplified polymorphism (S-SAP), Intron length polymorphisms (ILPs), Inter small RNA polymorphism (iSNAP), Direct amplification of length polymorphisms (DALP), Promoter anchored amplified polymorphism (PAAP), Target region amplification polymorphism (TRAP), Conserved region amplification polymorphism (CoRAP), Start Codon Targeted (SCoT) Polymorphism, and Directed Amplification of Minisatellite DNA (DAMD). Some molecular marker applications that have been recently employed to achieve various objectives in plant research have also been outlined. This review will serve as a useful reference resource for plant breeders and other scientists, as well as technicians and students who require basic know-how in the use of molecular or DNA marker technologies.

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“…Portanto as culturas de cártamo (Carthamus tinctorius L.) e níger (Guizotia abyssinica (L. f.) Cass) possuem potencial para serem utilizadas na produção de alimentos enriquecidos, fornecendo propriedades funcionais, pois constituem alimentos ricos em ácidos graxos monoinsaturados (ômega 3 e ômega 6), e atualmente sua inclusão em alimentos é pouco difundida, sendo utilizados apenas para fins industriais e produção de biodiesel (Ataide et al, 2018;Baghel et al, 2017;Beak et al, 2020;Kavyashree et al, 2015;Orgah et al, 2020) Dentro deste contexto, este trabalho teve como objetivo desenvolver biscoitos tipo cookie elaborados com farinhas de níger (Guizotia abyssintica (L. f.) Cass) e cártamo (Carthamus tinctorius L.) e caracterização em relação a composição proximal, atividade de água, peso, diâmetro e espessura, antes e após o forneamento e avaliação sensorial.…”

Section: Introductionunclassified

Elaboração De Sorvete a Base De Leite De Cabra, Adoçado Com Açúcar E/Ou Mel De Abelhas

Pinto¹,

Oliveira²,

Pessoa³

et al. 2021

Fundamentos E Atualidades Em Tecnologia E Inspeção De Alimentos, Volume 2

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Esta obra está licenciada com uma Licença Creative Commons Atribuição 4.0 Internacional (CC BY 4.0).O conteúdo desta obra e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva do autor. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos ao autor, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais.

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In vitro regeneration of Guizotia abyssinica Cass. and evaluation of genetic fidelity through RAPD markers

Cited by 10 publications

References 74 publications

Assessment of genetic stability on in vitro and ex vitro plants of Ficus carica var. black jack using ISSR and DAMD markers

Assessment of genetic stability on in vitro and ex vitro plants of Ficus carica var. black jack using ISSR and DAMD markers

Basic concepts and methodologies of DNA marker systems in plant molecular breeding

Elaboração De Sorvete a Base De Leite De Cabra, Adoçado Com Açúcar E/Ou Mel De Abelhas

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