Micropropagation of Sesbania aculeata (Pers.) by adventitious organogenesis

Abstract

Multiple shoots differentiated from hypocotyl explants of Sesbania aculeata (Pers.) syn S. cannabina (Retz.) Pers., a leguminous woody shrub, when cultured on Murashige and Skoog's basal medium supplemented with auxin (IBA, NAA) or auxin and cytokinin (IBA + BAP, NAA + BAP). Shoot budding occurred directly from the explant as well as from callus. Differentiation of shoot and root occurred in one step in the same concentration of auxin or auxin and cytokinin. Elongation of shoots occurred in the shoot induction medium.

Direct shoot organogenesis from hypocotyl explants of Jatropha curcasL. an important bioenergy feedstock

“…The ANOVA showed highly significant differences (P 0.05) between the concentration and combination of plant growth regulators tested for both mean shoot number and mean shoot length. Our present results are in accordance with the reported findings on many woody species where synergic effects of a higher concentration of cytokinin with a lower concen- tration of auxin induced better shoot organogenesis than in the medium supplemented with cytokinin alone (Bansal & Pandey, 1993;Reddy et al, 1995;Xie & Hong, 2001;Nayak et al, 2007;Behera et al, 2008).…”

Scenario of <i>in Vitro </i>Regeneration and Transformation Studies in Multipurpose <i>Sesbania </i>Species

“…Hypocotyls and cotyledons are the most commonly used explants for regeneration. De novo shoot organogenesis has been obtained either directly or via callus phase by using hypocotyl explants [9]. Almost every part of the plant can produce callus, but with variation in time period of formation, amount and nature of it [10].…”

“…The shoot bud regeneration from the callus varies with the explant type and concentration of growth regulators. Shoot organogenesis had reported from the callus derived from the leaves, cotyledons and hypocotyls [9,11] but not from the roots of the plants [10]. Direct shoot organogenesis with no intervening callus phase has reported using explants such as cotyledons, cotyledonary node, stem node and internodes [12][13][14].…”

“…There is only one report of in vitro regeneration in S. aculeata where differentiation of shoot buds occurs directly and indirectly on the same medium from hypocotyl and leaf explants [9]. The organogenesis depended on the combination of auxin and cytokinin in the medium.…”

Thiol Compounds, Pre-Conditioning and Orientation of Explants – Important Factors Affecting Regeneration from Cotyledons of Legume Crop Sesbania Aculeata

“…There is one report for regeneration in S. aculeata (Bansal & Pandey 1993) using hypocotyls and leaf explants, but the regeneration protocol is very time-consuming. In the current study, we have used mature cotyledon explants for regeneration which gave rise to multiple shoots in less time.…”