Background and Aim:Clenbuterol as a β2-agonist drug was investigated according to the concentration of the drug available in the bodies of goats and according to the level of sensitivity of the instruments used for detection. The objective of the current study was to determine withdrawal times after giving a therapeutic dose that resulted in safe slaughters.Materials and Methods:Five healthy male goats with a mean body weight of 20.64 kg were treated with a single dose of 5.10−3 mg/kg in the BW onto jugular vein. Whole blood samples of approximately 5 mL were taken in a time series at 5, 30, 60, 90, 150, 210, 270, 390, 510, 630, and 750 min. At 24 h posttreatment, all subjects were sacrificed, and 300 g samples of the liver were obtained. The plasma concentration and liver residue of the drug were observed by reverse-phase high-performance liquid chromatography.Results:The drug reached a maximum concentration of 19.233±0.331 µg/mL at 5 min, and the elimination half-life was at 173.25 min. The limit detection was obtained at 0.053 µg/mL. A one-way analysis of variance between all goats showed that elimination of the clenbuterol in their bodies was similar (p=1.00), with a withdrawal time of 1,479.326 min and no residues in the liver (p<0.05).Conclusion:Safe times for slaughter were determined to be at 2 days, 13 h, and 12 min as the 2nd safety factor (SF) time and 3 days, 1 h, and 58 min as the 3rd SF time with the liver organ free from residue.elimination half-life, new method for calculating withdrawal time, prescriptions for obtained β2-agonist, residues in liver.
Objective from this research was to determine the relationship of udder morphometry which consists of the height of the front udder, the height of the rear udder and the circumference ofudder on milk production in the Bima District of West Nusa Tenggara. One of ways to choose a good lactational mare is to pay attention at the shape of the part external body which is udder. The sample had used 32 mares that were in lactation period. Milk production has been measured from the average milk production per day in the lactation period. Milking process in Bima District, West Nusa Tenggara has done in three to four times a day in the span of four to five hours per day. Body length had been measured from os thoracalis to os coxae, chest circumference had been measured from os vertebrae thoracalis to os thoracalis, posterior abdomen of pelvic to os pelvis and the last of os lumbar vetebrae. The research design in this study was survey on every wild mares rancher. Data that has been collected and processed using Multiple Regression with SPSS program version 23th. The result showed that there was a strong relationship between height of rear udder and udder circumference to milk production with back udder with height coefficient of 0.21 (P <0.05) and udder circumference was 0.11 (P <0.05) and equation in Y = 2.423 - 0.398LA + 0.793TAB. However, there is no significant relationship between the height of the front udder and milk production.
Ractopamine hydrochloride often used as a bronchodilator, but its β-adrenergic agonist effects on un-striated muscle and its withdrawal time have not been assessed for Etawah goats and sheep. The aim of this study was to determine the safe time to slaughter goats and sheep post-treatment with ractopamine. Five clinically healthy adult goats and sheep (20 kg body weight) were treated with a single dose of ractopamine (1 mg, intravenously). Whole blood was sampled from the jugular vein at 120 min, 180 min and 300 min post-treatment. Ractopamine as a veterinary drug was analysed using HPLC at wavelength 225 nm.
Goats are small ruminants that are reared by the rural community for financial income and nutrition. This study was aimed to determine the effect of vitamin C addition in tris–egg yolk extender on the lifespan of Sapera goat spermatozoa stored in 5o C. Semen was collected from 1.5 years old Sapera male goat. The ejaculates were diluted in Tris-egg yolk (T-EY) extender added with 0, 0.2, 0.3, and 0.4 g vitamin C/mL extender for T0, T1, T2, and T3 groups, respectively. The spermatozoa motility, viability, and morphological abnormality were assessed along with storage at 5° C. The result showed that spermatozoa motility was the highest (p <0.05) in T1 compared to other groups and qualified for AI use for up to 72 hours. In 24 hours storage, the spermatozoa viability was not significantly different (p >0.05) among the groups. The spermatozoa viability in the T1 group was the highest (p <0.05) among the groups along 48-96 hours of storage. The spermatozoa morphological abnormalities of the T1 group was the lowest (p <0.05) compared to other treatment groups in the range of 24-72 hour storage. In the control group (T0), the less than 5% spermatozoa morphological abnormalities (qualified for artificial insemination) were only in the 24 hours storage, while those of the T1 group were up to 72 hours. It could be concluded that the addition of 0.2 g vitamin C/100 mL T-EY extender maintained the quality of Sapera goat semen for 72 hours at 5°C.
Penelitian ini bertujuan untuk menentukan konsentrasi gliserol yang optimal dalam pengencer tris dalam mempertahankan kualitas spermatozoa kambing Sapera. Penelitian ini menggunakan kambing Sapera jantan umur 1.5 tahun. Konsentrasi gliserol yang digunakan adalah 5%, 6% dan 7%. Semen ditampung dua kali dalam seminggu dengan menggunakan vagina buatan. Hasil penelitian menunjukkan rata-rata persentase motilitas dan viabilitas spermatozoa pada pengencer dengan gliserol 6% masing-masing 62.33% dan 75.83% terdapat perbedaan yang signifikan (p<0.05) dibandingkan pada pengencer dengan gliserol 5% masing-masing 57.67% dan 68.33% dan 7% masing-masing 59% dan 70.33%. Rata-rata abnormalitas pada pengencer dengan gliserol 5%, 6% dan 7% masing-masing 8%, 7.5% dan 7.67% tidak terdapat perbedaan yang signifikan (p>0.05). Dapat disimpulkan bahwa penambahan gliserol 6% dalam pengencer Tris berhasil melindungi spermatozoa dari cekaman dingin selama proses gliserolisasi, sehingga dapat mempertahankan motilitas, vibilitas dan abnormalitas spermatozoa.
Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak kedelai (Glycine max) terhadap jumlah pertumbuhan folikel ovarium pada mencit (Mus musculus). Sampel terdiri dari 20 ekor mencit yang secara acak dibagi menjadi lima kelompok. Kelompok kontrol (K) tidak diberi ekstrak kedelai. Kelompok P1 diberi ekstrak kedelai dengan dosis 0,05 mg/kgBB. Kelompok P2 diberi ekstrak kedelai dengan dosis 0,010 mg/kgBB. Kelompok P3 diberi ekstrak kedelai dengan dosis 0,015 mg/kgBB. Kelompok P4 diberikan ekstrak kedelai dengan dosis 0,020 mg/kgBB. Ekstrak kedelai diberikan selama 14 hari. Analisis data menggunakan uji One Way ANOVA dan dilanjutkan dengan uji Honestly Significant Difference (HSD). Hasil penelitian menunjukkan kelompok kontrol (K) memiliki perbedaan signifikan dengan kelompok P1, P2, P3 dan P4. Hasil rata-rata jumlah folikel primer tertinggi terdapat pada perlakuan 4 (P4) dengan dosis 0,020 mg/kgBB dan jumlah rata-rata folikel sekunder tertinggi pada perlakuan 3 (P3) dengan dosis 0,015 mg/kgBB. Penelitian ini dapat disimpulkan bahwa ekstrak kedelai yang diberikan pada mencit betina dapat meningkatkan jumlah pertumbuhan folikel ovarium dan jumlah rata-rata folikel tertinggi adalah pada perlakuan 3 (P3) dengan dosis 0,015 mg/kgBB.
Dairy cows farm business level of KUD Tani Wilis in Nyawang Kidul, Sendang district, Tulungagung city has some problems resulting low livestock productivity. One of the causes of low levels of dairy reproductivity is the incidence of repeat breeder. Repeat breeder is a condition of a female cow that failed to breed after being mated three or more times with a normal and healthy fertilized bull. The main purpose of this research is to know the risk factor of repeat breeder in dairy cows at farmer level of KUD Tani Wilis in nyawang kidul, Sendang district, Tulungagung city. The type of research is descriptive research. This research was using 32 dairy cows from 6 breeders, the selection of cattles used Simple Random Sampling method. The collection data using questionnaires and direct observations in farms resulted that the evidence rate of dairy cattle's repeat breeder in this research is 45.7%. The results also showed that the influence of knowledge of farmers on disease or reproductive disorders positively correlated with the evidence of repeat breader.
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