Objective: The aim of this study was to ascertain the effects of adding green tea extract (GTE) to skim milk-egg yolk (SM-EY) extender on both the quality of post-thawed bull semen and the pregnancy rates of the recipient cows.Methods: Twelve ejaculates from four Simmental bulls, aged 3 to 5 years and weighing 900 to 950 kg, were diluted SM-EY extender, added with 0, 0.05, 0.1, and 0.15 mg GTE/100 mL extender and then frozen. After four weeks storage in liquid nitrogen, the sperm were thawed and evaluated for viability, motility, intact plasma membrane (IPM), and DNA fragmentation. Meanwhile, the estrus cycles of 48 recipient cows were synchronized by intramuscular administration of a single injection of 5 mg prostaglandin F2α. Estrus cows were divided into four equal groups and inseminated artificially 18 to 20 h after the onset of estrus by using semen from each extender group. Pregnancy was diagnosed by measuring serum progesterone levels at 21 days, followed by transrectal palpation 90 days after insemination.Results: The findings revealed that adding 0.1 mg of GTE/100 mL extender produced the highest percentages of sperm viability (70.67%±1.75%), motility (69.17%±1.47%), and IPM (69.23%±1.21%) and the lowest percentage of DNA fragmentation (3.00%±0.50%). The pregnancy diagnosis revealed that all cows (36/36) inseminated using frozen semen in GTE addition extender were pregnant (pregnancy rate 100%), whereas the pregnancy rate of the control group was 83.33% (10/12).Conclusion: It may be concluded that 0.1 mg GTE/100 mL extender yields the best quality of spermatozoa and that all variants doses of GTE in extender produce a higher pregnancy rate among recipient cows.

The community in Kertosono Subdistrict has an average farming and trading livelihood. Almost all of the people who work as farmers have livestock that are kept as a side business or livelihood. Livestock that are kept include beef cattle, goats and sheep. The problems that arise include: The community has not fully understood how to obtain meat from the sacrifice of qurban animals with good quality. The public does not know of zoonotic diseases, and there are still not many people who know how the procedures for slaughtering livestock are good . The main objective in this community service activity is to be able to increase the knowledge and skills of the community regarding the criteria for ASUH meat (Safe, Healthy, Whole, and Halal) and appropriate handling and restraint on livestock.Abstrak

The main objective in this community service activity is to improve the insight of the people in Kepohbaru Bojonegoro sub-district about zoonotic diseases. The hope is that with the increasing knowledge of citizens, the level of public health in general can be better. The implementation method that will be used in this community service program is counseling and discussion. The purpose of counseling conducted to the community is to provide a general description of zoonotic diseases and then conduct an in-depth discussion about the clinical symptoms, handling, prevention and treatment of zoonotic diseases. After the activity is completed, monitoring will be carried out through the transfer of students of BBM KKN Universitas Airlangga.AbstrakTujuan utama dalam kegiatan pengabdian kepada masyarakat ini adalah dapat meningkatkan wawasan masyarakat di Kecamatan Kepohbaru Bojonegoro tentang penyakit zoonosis. Harapannya dengan bertambahnya pengetahuan warga, maka tingkat kesehatan masyarakat secara umum bias menjadi lebih baik. Metode pelaksanaan yang akan digunakan dalam program pengabdian masyarakat ini adalah penyuluhan dan diskusi. Tujuan dari penyuluhan yang dilakukan kepada warga masyarakat adalah memberikan gambaran umum tentang penyakit zoonosis dan selanjutnya dilakukan diskusi yang mendalam tentang gejala klinis, penanganan, pencegahan, dan pengobatan penyakit zoonosis. Setelah kegiatan selesai, akan dilakukan monitoring melalui penerjunan mahasiswa KKN BBM Universitas Airlangga.

This study addresses an advantageous application of a urinary zearalenone (ZEN) monitoring system not only for surveillance of ZEN exposure at the production site of breeding cows but also for follow-up monitoring after improvement of feeds provided to the herd. As biomarkers of effect, serum levels of the anti-Müllerian hormone (AMH) and serum amyloid A (SAA) concentrations were used. Based on the results of urinary ZEN measurement, two cows from one herd had urinary ZEN concentrations which were two orders of magnitude higher (ZEN: 1.34 mg/kg, sterigmatocystin (STC): 0.08 mg/kg in roughages) than the levels of all cows from three other herds (ZEN: not detected, STC: not detected in roughages). For the follow-up monitoring of the herd with positive ZEN and STC exposure, urine, blood, and roughage samples were collected from five cows monthly for one year. A monitoring series in the breeding cattle herd indicated that feed concentrations were not necessarily reflected in urinary concentrations; urinary monitoring assay by ELISA may be a simple and accurate method that reflects the exposure/absorption of ZEN. Additionally, although the ZEN exposure level appeared not to be critical compared with the Japanese ZEN limitation in dietary feeds, a negative regression trend between the ZEN and AMH concentrations was observed, indicating that only at extremely universal mycotoxin exposure levels, ZEN exposure may affect the number of antral follicles in cattle. A negative regression trend between the ZEN and SAA concentrations could also be demonstrated, possibly indicating the innate immune suppression caused by low-level chronic ZEN exposure. Finally, significant differences (p = 0.0487) in calving intervals between pre-ZEN monitoring (mean ± SEM: 439.0 ± 41.2) and post-ZEN monitoring (349.9 ± 6.9) periods were observed in the monitored five cows. These preliminary results indicate that the urinary ZEN monitoring system may be a useful practical tool not only for detecting contaminated herds under field conditions but also provides an initial look at the effects of long-term chronic ZEN/STC (or other co-existing mycotoxins) exposure on herd productivity and fertility.

Dairy cows farm business level of KUD Tani Wilis in Nyawang Kidul, Sendang district, Tulungagung city has some problems resulting low livestock productivity. One of the causes of low levels of dairy reproductivity is the incidence of repeat breeder. Repeat breeder is a condition of a female cow that failed to breed after being mated three or more times with a normal and healthy fertilized bull. The main purpose of this research is to know the risk factor of repeat breeder in dairy cows at farmer level of KUD Tani Wilis in nyawang kidul, Sendang district, Tulungagung city. The type of research is descriptive research. This research was using 32 dairy cows from 6 breeders, the selection of cattles used Simple Random Sampling method. The collection data using questionnaires and direct observations in farms resulted that the evidence rate of dairy cattle's repeat breeder in this research is 45.7%. The results also showed that the influence of knowledge of farmers on disease or reproductive disorders positively correlated with the evidence of repeat breader.

AbstrakTujuan penelitian ini untuk menterminasi peningkatan kandungan nutrien dalam fermentasi makanan yang terdapat dari kulit kacang sebagai bahan pakan alternatif untuk ternak dan ikan. Penelitian ini terdiri dari tiga perlakuan dan lima replikasi P0: kulit kacang+isolat 0%; P1: kulit kacang+1% Lactococus sp. ; P2 : kulit kacang+1% Lactobacillus sp. Proses fermentasi dilakukan selama 5 hari dalam keadaan aerob. Hasil menunjukan bahwa terdapat berdedaan yang signifikan antara perlakuan (p<0,05). Penggunaan isolat Lactococcus sp. dan Lactobacillus sp. dapat meningkatkan kandungan nutrient dari limbah kulit kanang. Terdapat penurunan aerob mentah dari 3,80 sampai 7,70% terdapat peningkatan elestrak nitrogen bebas dari 3,925 sampai 4,38% terdapat peningkatan kandungan energi pencernaan 7.13% sampai 9.30%. berdasarkan hasil penelitian dapat disimpulkan bahwa pengguna 1% Lactococcus sp. dan 1% Lactococcus sp. dapat meningkatkan nilai nutrisi di limbah kulit kacang yang dapat digunakan sebagai alternatif bahan pakan ternak dan ikan.                                                   AbstractThis study aims to determine the increasing of nutrient content in the fermentation of feed stuff derived from peanut wastes as an alternative feed material for livestock and fish. The study consisted of three treatments and five replications, namely P0: peanut peel + 0% isolate, P1: peanut peel + 1% Lactococcus sp, P2: peanut peel +1% Lactobacillus sp. The fermentation process was carried out for 5 days under a facultative anaerobic condition. The results showed that there was a significant difference between treatments (p <0.05). The use of Lactococcus sp and Lactobacillus sp isolates can increase the nutritional content of peanut peel wastes. There was a decrease in crude fiber content (CF) of 3.80 to 7.70%, there was an increase of Nitrogen free extract (NFE) of 3.925 to 4.38%, there is an increase in digestible energy (DE) content of 7.13% to 9.30%, there was the increasing metabolizable energy (ME) of 7.13% to 9.12% compared to control. Based on the results it can be concluded that the using of 1% Lactococcus sp and 1% Lactobacillus sp. can increase nutritional value of peanut peel waste that can be used as an alternative ingredients of animal and fish.

The purpose of this research is to identify lice and to know the pattern of infestation of lice that infest laying ducks in Village of Kramat, District  of Bangkalan, Region of Bangkalan. Forty samples of laying ducks were taken in Kramat Village and identified in Entomology and Protozoology Laboratory of Parasitology Department of Veterinary Faculty of Airlangga University, the research was conducted from July to September 2017. This study used an explorative observational research design. Lice taken from a sample of laying ducks are fed into an ointment pot containing 70% alcohol to preserve lice and labeled in accordance with the infected duck's limb region. Identification of lice using Permanent mounting method without coloration. The results of identification, the lice that infest laying ducks consists of three types of Anaticola crassicornis 60%, Menacanthus stramineus 25% and Lipeurus caponis 17.5%. The results of infestation pattern, infected body's region is head-neck, wings and back. The wings infested two types of lice were A. crassicornis and L. caponis, head-neck infested M. stramineus and in the infected back region of A. crassicornis.

Background and Aim: We previously reported the mitigation effects of difructose anhydride III (DFA III) on mycotoxins, such as zearalenon and sterigmatocystin, based on the urinary concentrations of these molecules in calves. This study was aimed at evaluating the effects of dietary supplementation of DFA III and the fermented status of DFA III in the intestine by comparing serum levels of short-chain fatty acid (SCFAs) in DFA III-supplemented cattle with those in non-supplemented control cattle. Materials and Methods: Serum SCFA concentrations were measured in 30 Japanese Black heifers, aged 9–10 months, from two herds, using gas chromatography on days 0 (before DFA III supplementation), 9, and 14 after DFA III supplementation. Results: A notably different trend was observed for isobutyric acid and enanthic acid, which may reflect the different fermentation status of supplementary DFA III in the intestine. Our results indicate the possibility that this trend observed in the intestinal tract following DFA III administration is associated with changes in the environment of intestinal bacterial flora, which may partially reflect the effects of DFA III supplementation on cattle. Conclusion: Difructose anhydride III supplementation for at least 2 weeks affects the trend of blood SCFA concentrations in cattle. Our results provide evidence supporting the effects of DFA III on the intestinal environment and intestinal barrier function. Keywords: cattle, difructose anhydride III, short-chain fatty acid.