Aim:The current study was conducted to evaluate the effect of platelet-rich fibrin matrix (PRFM) treated with silver nanoparticles (AgNPs) on enhancing the healing of the experimentally induced bone gap in a rabbit model.Materials and Methods:Twenty healthy male local rabbits aged between 6 and 8 months, their weights between 1.5 and 2 kg were used in this study and divided randomly into four equal groups, under general anesthesia (1 cm), bone gap was induced in the tibia bone to create a critical bone defect and leave it without any treatment in the first group (control group). While in the second group the bone gap was filled with PRFM; in the third group, the gap was filled with 0.3 ml AgNPs; and in the fourth group, the gap was filled with PRFM treated with AgNPs.Results:There was no infection at the operation site in all experimental animals, and the radiograph images showed periosteal and endosteal reaction; the gaps were bridged faster in the fourth group as compared with the other groups. The histological examination showed lamellar bone with haversian canal completely filled the fracture gap and contact with old bone in the fourth group as compared to other groups.Conclusion:Using a combination of PRFM and single nucleotide polymorphisms together gave better acceleration in the bone healing process than using each one of them separately.
Objective: This study was designed to identify the effectiveness of platelet-rich plasma gel (PRP) and platelet-rich fibrin matrix (PRF) application on the induced open chronic full-thickness cutaneous wounds in goats.Methods: A total of 24 adult apparently healthy local breed bucks were used in this study. PRP gel and PRF matrix were prepared from whole blood at the time of treatment, four square full-thickness skin wounds (4 cm × 4 cm) on the dorsal sides of the back of each animal were created, by daily scratched to interrupt healing process continuation to prolong inflammatory reaction to form chronic wound, this surgical procedure was continued for 8 weeks to ensure the traditional chronicity wounds, after that they were treated by PRP gel in the first group (n=12), while PRF matrix was used in the second group (n=12). Each group was also subdivided into treatment (n=2 wounds) and control group (n=2 wounds) in each animal. The healing process was evaluated by clinical examination during experimental period and histologically in day 7, 14, 28, and 45 post operation. Conclusion:Both groups showed faster tissue repair and epithelialization in treatment groups compared to control groups. The results of this study confirmed PRP gel and PRF matrix application improved and accelerated cutaneous open chronic wound healing.
| This study was planned to evaluate the efficacy of two biological matrices represented by autologous platelet rich fibrin matrix (PRFM), as well as a cross linked decellularized caprine pericardial extracellular matrix (PECM) on enhancing healing of the experimentally severed superficial digital flexor tendon in a goat model. It was carried out on 48 adult bucks, which were divided randomly into three equal groups. Under sedation and regional anesthesia, SDFT was severed at the mid metacarpal region of the right forelimb. In the first control group, tenorrhaphy was performed and left without additives. While in the second group the tenorrhaphy site was wrapped with autologous platelet rich fibrin strips which were prepared at the time of operation, as well as in the third group the tenorrhaphy site was wrapped with a cross linked decellularized PECM strip which was previously prepared from the whole fresh caprine pericardium. The histopathological evaluation in this study revealed that the neovascularization, cellularity and collagen fiber alignment scores were significantly increased in both treated groups as compared to the control group. In conclusion, both biological matrices led to improvement in tendon healing and repair, without any complications, but the PECM gave superior results in tendon repair when compared to other groups.
| The present study was carried out to isolate and identified the Staphylococcal-specific bacteriophage from swage water by using agar overlay method and to investigate the anti-Staphylococcal activity of Isolated Bacteriophage in-vitro. Two experiments were performed in this present study; the first one was isolation and identification of Methicillin-Resistant Staphylococcus aureus (MRSA) from post-traumatic bone infection. While the second experiment included study the infectivity of bacteriophage in-vitro to MRSA as well as effect of temperature and pH on the infectivity of phage was also studied. Results of antimicrobial susceptibility test of S. aureus isolates revealed that most of isolates were resistant to Methicillin, Cefoxitin and Pencillin G. Staphylococcus aureus phage was isolated from sewage samples by agar overlay method, the phage was characterized by clear, circular plaques ranged between 2-3 mm in diameter. TTA method for determined the Phage MIC appears to be both reliable, and reproducible. This method is laborious and time consuming, and the proposed MIC method appears to be an interesting alternative. Keywords
This study aimed to investigate the effect of non-thermal plasma (NTP) with Fetal caprine acellular dermal matrix (FCADM) scaffold in healing of the full-thickness acute skin wound in bucks. eighteen apparently healthy adult local breed bucks aged 1-2 years and weighing 25-30 kg were used. Each animal was subjected to creation of 4 full-thickness acute skin wounds on the shoulder region, two wounds on each side 10cm apart, the animals were divided into 3 groups, at first control group wounds were treated in routine way (cleaning the wound with normal saline every other day and dressing). The second group the wounds were treated with Fetal caprine acellular dermal matrix. The third group skin wounds were exposed to non-thermal plasma treatment with dose 6.6W/cm² for 30 sec (used probe 1cm in dimeter), then application of Fetal caprine acellular dermal matrix. The wounds were histopathologically evaluated at 3,7,21, 35 days post-operation using Hematoxylin & Eosin (H& E) and mason’s trichrome stain.
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